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STEM WOrkplace Practices Q3
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- 6) You want to determine the amount of cells in a culture. You dilute the suspension to 10-4 and plate 100ul onto an agar plate. After overnight incubation there are 30 colonies on your plate. How many cells are in your original suspension (assume your culture is 1 Liter)?1.Describe the difference between the appearance of surface and subsurface colonies in a pour plate. If this is the same bacterial species, why do these differences in colonial growth occur? 2.Define the term "colony" as it relates to bacterial growth on solid media.10. A single cell is placed into a culture tube containing nutrient agar. If the number of cells triples every 2 minutes and the culture tube is completely filled in 12 hours, how long does it take for the culture tube to be only half full of cells?
- 1. What are the basic differences from a primary cell culture, cell line and a cell strain? 2. Differentiate the five classes of artificial media used in animal cell culture. 3. What are some of the common problems encountered when performing cell culture?15. Agar is used for solidifying culture media because: it does not affect the growth of bacteria it does not add to the nutritive properties of the medium the melting and solidifying points of agar solution are not the same all of the abovent ● ● S ● Paragraph F 2. To determine if the type of agar affects bacterial growth, a scientist cultures on fou different types of agar. Five petri dishes are set up to collect results: One with nutrient agar and E. coli One with mannitol-salt agar and E. coli One with MacConkey agar and E. coli One with LB agar and E. coli Styles One with nutrient agar but NO E. coli Ed
- 1. TRUE or FALSE. Relative to a 24 hour culture of endospore forming bacteria, a 72 hour culture would contain more endospores.1. List multiple reasons why preventing the growth of certain microbes is necessary/valuable 2. For bacterial growth, what can we do to compose a continuous culture where we would get continuous supply of the product? 3. Antibiotics - How did we compose major issues for treatment through resistance built up from the antibiotics?5. Give two reasons for why heat fixing is used? Adherence of bacterial cells to the slide Denaturing of bacterial enzymes to preserve cellular integrity
- 1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.1. What is the purpose of preparing a cell culture?2. Differentiate the general types of cell culture.3. Enumerate and describe the role of the different components of a cell culture media.Explain the statements below: 1.Animal cell cultures requires high technical skills to interpret and to regulate animal cell culturing. 2. Animal cell culture makes use of low amounts of reagents.