2. To track the insulin purification process, the ratio of insulin to total protein is tracked through each purification step. Graph how you would expect that ratio to change over the course of the purification process (assume that there are three discrete steps to the purification process).

Please dont provide handwrittin solution.../.

VI. Although most biologically relevant sugars have the D-configuration, L-fucose is a commonly observed monosaccharide component in glycoproteins. Glycosylated antibodies (IgGs) observed in patients with active TB have more fucose than those found in patients with latent TB (Lu et. al., 2016). Latent TB occurs when a person has the TB bacteria, but bacterial proliferation is kept under control by the body's immune system; hence, the infected person is asymptomatic and not infectious. A person with latent TB will test positive for the tuberculin test or TB blood test. a) L-fucose is also known as 6-deoxy-L-galactose. Note that D-galactose is a C-4 epimer of D-glucose. Draw the Fisher projections for D-glucose, D-galactose and L-fucose. (Hints: D- and L-isomers are enantiomers. 6-deoxy means that the hydroxyl group at C-6 is removed leaving just the methyl group). D-galactose: D-glucose: L-fucose (6-deoxy-L-galactose): b) L-fucose can cyclize into a pyranose and form two anomers. Draw…

make a scatter plot with the given criteria and data.  use excel.

Could you please help answer this question?

. Studies on CAMP actions in cultured cells usually involve adding to the cell culture not CAMP, but dibutyryl CAMP (see structure). Why is this structural modification necessary? How could you test the premise that di-Bu-CAMP has the same biochemical effects as CAMP? N. N= Nat Dibutyryl CAMP O

1. Make a Lineweaver-Burk plot and use the plot to complete the information in the table and the following questions.  a. Is it possible for the enzyme to overcome the effect of the inhibitor in question from the chart. Explain.  b. What prevents this enzyme from being an even more catalytically efficient enzyme? c. What do single molecule data indicate about the validity of ensemble data?d. What is the reason that humans are insensitive to sulfa drugs?

4. Below is a Michaelis-Menten plot for a wild-type (WT) and mutant (V105A) enzyme isolated from the bacterium Staphylococcus aureus. The enzyme is involved in carbohydrate metabolism and is a potential biocatalyst for the large-scale production of rare sugars. (8) mg V₂ μmol s1 1.50- 1.25- 1.00- 0.75- 0.50- 0.25- 0.00 0 100 200 300 [s], mM 400 500 WT (a) Estimate the KM and Vmax for the wild-type and mutant enzyme from the graph. V105A (b) Calculate the keat and keat/KM for the wild-type and mutant enzyme based on your estimated values in (a) if the total enzyme concentration is 0.5 µmol/mg. (c) Is the mutant enzyme a more or less efficient catalyst than the wild-type enzyme? Briefly explain.

Proteases are one of the main drug targets. Choose the False statement regarding proteases. A. Proteases are enzymes that cleave peptide bonds. B. Water is a reactant in the reaction catalyzed by proteases. C. Proteases, like all enzymes show substrate specificity, meaning they cleave only substate that fit the bonding product. D. Proteases rely on the proton transfer from NADH to the substrate. E. Protease mechanism involves only acid-base catalysis.

Patients with a form of early-onset diabetes were found to carry a variety of mutations reducing the function of the glucokinase (GK) enzyme. These patients are heterozygous for one of the mutations, and thus show only partial rather than complete loss of GK activity. Answer the following questions about these patients. 1. How would blood sugar levels likely be affected in GK-mutation patients? Briefly explain your choice (25 words or less) a. Higher than normal b. Normal C. Lower than normal d. Cannot predict based on the provided information