2. Determine the size of each cell in the diagrams below using the ACTUAL SIZE OF THE CELL FORMULA. The diameter of the FOV has been given. Estimate the # of cells that fit across the FOV in the three examples. (Use the length of the cell for your estimation) Diameter of FOV = 1400 µm Diameter of FOV = 550 µm Diameter of FOV = 1200 µm Estimate # of cells to fit across FOV: Estimate # of cells to fit across FOV: Estimate # of cells to fit across FOV: Size = Size = Size =

15. After an endospore stain, you observe pinkish red cells which means a. these cells were producing ATP b. these cells were not producing ATP c. these cells were lacking an energy source d. these cells were water deficient e. a & c 16. Red blood cells (RBCs) do not contain mitochondria. Based off of this information, please answer the following question. Which of the following would indicate the presence of RBCs in the media? a. a blue simmon's citrate test tube b. a purple decarboxylase test tube c. a yellow mr test tube d. a yellow glucose test tube e. a red vp test tube 17. The role of crystal violet in the gram stain is similar to the role of acid-fast stain. a. methylene blue b. carbolfuschin c. acetone alcohol d. heat e. acid alcohol lased th Time ou in the

8. Tube number 1 contains a broth culture of bacteria. You transfer 30ml from tube 1 and place it into tube 2, which contains 270 ml of diluent. Calculate the dilution performed to create tube 2. What is the dilution factor between tubes 1 and 2? b. If the concentration of bacteria in tube 2 is 2x106 cells/ml, what is the concentration of a. bacteria in tube 1? с. If tube number 1 contains 5ml of bacterial culture, what is the total number of bacterial cells present in tube 1? d. What is the total number of bacterial cells in tube 2?

5. The proteins listed below are separated by (1) isoelectric focusing (IEF) followed by (2) sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). A single sample of the protein mixture is placed at the top center of the gel to begin step 1. ID 1 2 3 4 5 6 7 8 Protein Collagen y-Globulin Insulin Fibronectin Lysozyme Pepsin Serum Albumin Myoglobin Molecular Weight 300,000 150,000 5,700 220,000 14,300 34,500 66,500 16,700 Conc pl 0.5 mg/L 4.7 1 mg/L 7.2 12 mg/L 5.3 5.8 11.0 5 mg/L 2 mg/L 10 mg/L - added prior to running IEF? left out of the process entirely? 4 mg/L 2 mg/L 1.0 4.9 7.1 A. Which of these proteins would electrophoresis NOT separate effectively? B. Which of these proteins would isoelectric focusing NOT separate effectively? Type fibrous a-helices globular protein globular protein fibrous protein enzyme digestive enzyme blood protein muscle protein C. Show the expected results after each step, by drawing qualitative diagrams similar to those shown in the class…