2) Two students attempted to estimate the extinction co-efficient of NADH by measuring the absorbance of different concentrations at 340nm. The data was plotted and a linear regression fitted 0.35 0.30 Replicate 1: Absorbance = 6.32 x [NADH] (mM) 0.25 0.20 0.15 0.10 0.05 Replicate 2: Absorbance = 2.80 x [NADH] (mM) 0.00 0.00 0.01 0.02 0.03 0.04 0.05 0.06 NADH concentration (mM) Figure 1: Estimation of the extinction co-efficient of NADH. The absorbances of different concentrations of NADH were measured at 340nm. Linear regressions were fitted to the two data sets, with the equations displayed. i) Describe the differences between the two replicate standard curves. ii) Speculate on a possible reason that may explain the differences between the two replicates? Absorbance (340nm)
2) Two students attempted to estimate the extinction co-efficient of NADH by measuring the absorbance of different concentrations at 340nm. The data was plotted and a linear regression fitted 0.35 0.30 Replicate 1: Absorbance = 6.32 x [NADH] (mM) 0.25 0.20 0.15 0.10 0.05 Replicate 2: Absorbance = 2.80 x [NADH] (mM) 0.00 0.00 0.01 0.02 0.03 0.04 0.05 0.06 NADH concentration (mM) Figure 1: Estimation of the extinction co-efficient of NADH. The absorbances of different concentrations of NADH were measured at 340nm. Linear regressions were fitted to the two data sets, with the equations displayed. i) Describe the differences between the two replicate standard curves. ii) Speculate on a possible reason that may explain the differences between the two replicates? Absorbance (340nm)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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