2) A few years ago, a graduate student in my lab realized that a DNA construct in which he fused the coding sequence for one protein (the 'Myr-tag') to the coding sequence for another protein ('Kibra'), with a short linker between, was not working properly. The goal of this DNA construct was to express a 'fusion protein' which had the Myr-tag fused to the amino terminal end of the Kibra protein. Looking at the portion of the sequence shown below where the two proteins are fused, why did this experiment fail? The brackets above the DNA sequence show the codons corresponding to the amino acids in the proteins shown below the DNA sequence. The translation start site for the fusion protein is indicated. Translation starts,here 5' CACCÀTGggcaacaaa'tgc'tgcagcaagcgacaggcGCCACCATGccGAAtCTGCACAAAccGcATCGCAATCGCAG 3' iGTGGTACccgttgtttacgacgtcgttcgctgtccgCGGTGGTACGGCTTAGACGTTGTTTGGCGTAGCGTTAGCGTCı _5 Met Gly Asn Lys Cys Cys Ser Lys Arg Gln, Myr tag 1 10 1 Met Pro Asn Leu Gin Gin Thr 10 Ala Ser Gln Ser Gln Linker Kibra

The table shows the partial sequences of a wild type polypeptide and three mutant polypeptides as well as the type of single nucleotide mutation that produced each mutant polypeptide. Peptide sequence Met - Leu - Arg - Ile - ... Type of mutation Wild type Met - Leu - Arg - Met - ... Met - Leu - [STOP] Mutant 1 transition Mutant 2 transversion Mutant 3 Met - Phe - Arg - Ile - ... transition Determine the mRNA sequence for the wild type polypeptide by identifying the codons that correspond to each amino acid. The first codon has been filled in for you. Codon information can be found in the codon access table. Met Leu Arg Ile Answer Bank CỦA AUA CGU CGA AUG AUC AGA UUG CÚC

The following represent deoxyribonucleotidesequences in the template strand of DNA:Sequence 1:         5'@CTTTTTTGCCAT@3'Sequence 2:         5'@ACATCAATAACT@3'Sequence 3:         5'@TACAAGGGTTCT@3'(a) For each strand, determine the mRNA sequencethat would be derived from transcription.(b)  determine the amino acidsequence that is encoded by these mRNAs.(c) For Sequence 1, what is the sequence of the codingDNA strand?

3b) In the real world, where "wobble" pairing is possible, what is the minimum number of tRNAs required to service all of the threonine codons? Write out the base sequences of the anticodons of those tRNAs (remember to label the 5' and 3' end of each anticodon sequence).