17. In gene sequencing, the different sized fragments made visible in a gel electrophoresis are created by while in a restriction digest, the different sized fragments made visible in a gel electrophoresis are created by a. restriction enzymes; stop nucleotides b. DNA polymerase; restriction enzymes c. go nucleotides; restriction enzymes d. stop nucleotides; DNA polymerase e. stop nucleotides; restriction enzymes 16 EDNA placu fter 6 cycles of PCR 4444.

17. In gene sequencing, the different sized fragments made visible in a gel electrophoresis are created by while in a restriction digest, the different sized fragments made visible in a gel electrophoresis are created by a. restriction enzymes; stop nucleotides b. DNA polymerase; restriction enzymes c. go nucleotides; restriction enzymes d. stop nucleotides; DNA polymerase e. stop nucleotides; restriction enzymes 16 EDNA placu fter 6 cycles of PCR 4444.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 1TYK

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2. Which of these statements is INCORRECT about restriction enzymes Restriction enzymes form phosphodiester bonds when DNA are assembled Palindromic sequences are recognized by restriction enzymes Restriction enzymes recognize blunt ends Restriction enzymes recognize sticky ends Restriction enzymes originated from bacteria
FIGURE 2 shows the only suitable DNA restriction site in a plasmid DNA vector that can be cleaved. 5'.GTAATCCGGATCCGAAATCCCCCGG... 3' 3.CATTAGGCCTAGGCTTTAGGGGGCCC... 5 FIGURE 2 Identify the most suitable enzymeto be used as a restriction enzyme forthis purpose. O Ligase O Smal O EcoRI О ЕсoRV
1. What are restriction enzymes and what do they do? 2. Complete the chart Original DNA sequence 5' TGA CCA CTC GAG CAT AAC GAG TCG CTC 3° Write the complementary strand's sequence * remember it will be opposite directionality (the number at the ends are different) * pairs are A-T and C-G |Copy and paste your double stranded DNA into this box. You are going to use the restriction enzyme Xhol. Mark the cut site by changing the color (highlight or change text color) of one double-stranded piece * it cuts between the C and the T (cut shown as slash symbol): 5’ C/TC GAG 3' * remember it cuts BOTH strands (the restriction site is palindromic). Hint: read each strand in the 5'à 3' directions to find the cut site
Some Steps Involved in Creating Recombinant DNA 1. Plasmid DNA is cut at the restriction site.2. Foreign DNA is cut at the restriction site.3. Plasmid DNA is opened and sticky ends are formed.4. Foreign DNA restriction fragments are isolated5. Target sequence in the plasmid is recognized by the restriction endonuclease.6. Target sequences in the foreign DNA are recognized by the restriction endonuclease.7. Foreign DNA restriction fragment is inserted into the plasmid at the restriction site.8. DNA ligase splices the foreign restriction fragment and the plasmid together. Identify the correct sequence of steps involved in cutting and reassembling DNA molecules to make recombinant DNA. Start with preparation of the plasmid DNA. Select one: a. 6, 2, 4, 7, 8, 5, 1, 3 b. 5, 1, 3, 6, 2, 4, 8, 7 c. 5, 1, 3, 6, 2, 4, 7, 8 d. 6, 2, 4, 5, 1, 3, 8, 7
1. What are restriction enzymes.2. Give 5 restriction enzymes and its source.3. What is a restriction map.4. Explain each step used in restriction mapping.STEP 1: Preparation of DNA for restriction analysis.STEP 2: Restriction digestion of DNA.STEP 3: Separation of restricted DNA.STEP 4: Collecting data.STEP 5: Construction of restriction map
1. A plasmid was digested with two restriction enzymes, individually and in a double digest. The following fragment sizes (bp) were determined by agarose gel electrophoresis. Restriction Enzyme|Fragment Size (bp) 30, 15, 5 HindIII 50 EcoRI + HindIII 20, 15, 10, 5 Make a restriction map of the plasmid based on this data and indicate the distances between the restriction sites.
18. An instructor had her students perform this laboratory beginning with setting up their own restriction enzyme digests. One team of students had results that looked like those below. What is the most likely explanation for these results? DNA DNA Uncut DNA Hind III Eco RI a. The students did not allow enough time for the electrophoresis separation b. The agarose preparation was faulty c. The methylene blue did not stain the DNA evenly. d. The restriction enzyme EcoRI did not function properly. e. The voltage was set too low on the apparatus.
Part A Which of the following statements concerning restriction enzymes is true? Select all that apply. ►View Available Hint(s) ☐ Restriction enzymes specifically target and cut RNA in a sequence-specific manner. Restriction enzymes occur naturally in viruses as a defense mechanism against bacteria. Some restriction enzymes generate overhangs in the target DNA sequence upon incubation. During a cloning experiment, the vector and target DNA should be cut with different restriction enzymes to ensure that sticky ends are generated. Submit
1. (a) Restriction sites are usually ______. Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences (b) Involves joining a donor DNA fragment of interest to a vector Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences
DNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes Exo nucleases O DNases
The Centers for Disease Control and Prevention (CDC) program PulseNet uses genomic fragments that have been generated by restriction endonucleases O pathogen specific primers O PCR combined with restriction fragment length polymorphism (RFLP) analysis O oligonucleotide probes in microarray technology
27. You have a DNA sequence of 1400 bp. To characterize it, you decide to make a restriction map. Cutting with the first enzyme, you show by electrophoresis that the fragments produced are 400 and 1000 bp, respectively. After using the second enzyme, you recover 600-bp and 800-bp fragments. After treatment with both enzymes, fragments were 200, 400, and 800 bp. Draw the correct restriction map.