10-20. Find the pH and the concentration of each species of lysine in a solution of 0.010 0 M lysine ·HCI, lysine monohydrochloride. The notation "lysine HCl" refers to a neutral lysine molecule that has taken on one extra proton by addition of one mole of HCl. A more meaningful notation shows the salt (lysineH+)(Cl¯) formed in the reaction.

2.7 Now that you have figured out the reaction mechanism, let's explore the action mechanism of the following drug. Notice that this molecule is composed of two chloroethylamine groups and therefore can react with two different nucleophiles. Complete the following reaction mechanism. R-N Cl CL + R'-NH₂ R"-NH₂ The above reaction is responsible for the activity of the drug. Because of its ability to react with two nucleophiles, the drug can create a bridge between two biologically active molecules and disrupt their activity. This process is known as 'cross-linking'. For example, it can react with two DNA strands and prevent their separation during replication. This will cause cytotoxicity and cell death. 2.8 Draw a scheme/cartoon to represent the general idea of chemical cross-linking described above.

Good evening. I hope you are well. My chemistry question is attached to this message as a JPG.

What is this product?

Help please

Leu 93  Val 49. Are these two residues polar or hydrophobic (nonpolar)? What effect should their proximity have on the pKa Lys 216 Schiff base?

4d

23

Can you draw the following. Around what's it suppose to look like. 1.)  Clean Surface: [Gold surface of SPR]• SAM Formation: Gold surface - S - (CH2)11 - OH• Activation: Gold surface - S - (CH2)11 - CHO (using PCC)• Carbohydrate Coupling: Gold surface - S - (CH2)11 - CH=N - Carbohydrate (followed by NaBH3CN reduction)   2.) Clean Surface: [Gold surface of SPR]• SAM Formation: Gold surface - S - (CH2)11 - OH• Activation: Gold surface - S - (CH2)11 - CHO (using PCC)• Carbohydrate Coupling: Gold surface - S - (CH2)11 - CH=N - Carbohydrate (followed by NaBH3CN reduction)

N-acetylphenylalaninamide 2. (a) is a substrate of a-chymotrypsin. Draw the structural formula of this molecule, indicate which bond is cleaved in the hydrolytic reaction, and explain from its structure why it is a specific substrate of a-chymotrypsin. What influence does the acetyl group have on the reaction? (b) The table on the right lists initial velocity data for hydrolysis of N-acetylphenylalaninamide catal- yzed by a-chymotrypsin. From the data calculate the value of KM by a graphical method using a rectangular hyperbolic function to relate velocity to substrate con- centration and compare the results to the value obtained by a linear least-squares fit of the data in the form of a double-reciprocal plot. Substrate conc. (mm) 10.5 16.1 20.0 35.0 93.4 Vo (mmole/min) 3.33 x 10-² 4.55 x 10-² 5.56 x 10-² 7.15 x 10-² 10.0 x 10-²