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1. TRUE or FALSE. An autoclave can be used to sterilize growth media?
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- 1. Briefly define culture medium. 2. What is the principle behind sterilizing media before use and before disposal? 3. Why are plated media stored in an inverted position?1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.
- You have a broth medium that you would like to turn into a solid medium in order to do streak plates. What could you add to your media to allow it to solidify? Paragraph В I UUV light is used in clean rooms and some operating rooms. what are the limitations of using UV light as a means of sterilization?Illustrate the different solid tubed media formed 1. Deep/Butt solid tubed media2. Slant solid tubed media 3. Butt-slant solid tubed media
- 1. In terms of counting cells, what does a plating method accomplish that an electronic cell counting method does not? 2. Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase? 3. Some hyperthermophiles can survive autoclaving temperatures. Are they a concern in health care?48. Selective media is used to distinguish one microorganism type from another growing on the same media : A. False. B. True.1. What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame? 2. Why is heat a highly effective sterilizing agent?3. Which of the three methods of heat sterilization is the most appropriate and practical to use in sterilizing antibiotic solutions? (Direct flame, Dry heat, Moist heat)