1. You were fortunate to use a pre-packed column in this experiment. List two methods you could use to pack a column should you ever need to do it yourself. 2. Each of the following could lead to poor liquid chromatographic separation. Explain how or why each one would decrease the separation of two molecules. a. collection of large elution fractions b. very slow flow rate of mobile phase c. too weak of an adsorbent 3. When 2-propanol was used as the developing solvent, two substances moved with the solvent front (R₁ = 1) during TLC analysis on a silica gel plate. Can you conclude that the two compounds are identical? If not, what additional TLC experiment(s) would you perform to investigate further?

1. You were fortunate to use a pre-packed column in this experiment. List two methods you could use to pack a column should you ever need to do it yourself. 2. Each of the following could lead to poor liquid chromatographic separation. Explain how or why each one would decrease the separation of two molecules. a. collection of large elution fractions b. very slow flow rate of mobile phase c. too weak of an adsorbent 3. When 2-propanol was used as the developing solvent, two substances moved with the solvent front (R₁ = 1) during TLC analysis on a silica gel plate. Can you conclude that the two compounds are identical? If not, what additional TLC experiment(s) would you perform to investigate further?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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