Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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1. You were fortunate to use a pre-packed column in this experiment. List two methods you
could use to pack a column should you ever need to do it yourself.
2. Each of the following could lead to poor liquid chromatographic separation. Explain how
or why each one would decrease the separation of two molecules.
a. collection of large elution fractions
b. very slow flow rate of mobile phase
c. too weak of an adsorbent
3. When 2-propanol was used as the developing solvent, two substances moved with the
solvent front (R₁ = 1) during TLC analysis on a silica gel plate. Can you conclude that the
two compounds are identical? If not, what additional TLC experiment(s) would you
perform to investigate further?
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Transcribed Image Text:1. You were fortunate to use a pre-packed column in this experiment. List two methods you could use to pack a column should you ever need to do it yourself. 2. Each of the following could lead to poor liquid chromatographic separation. Explain how or why each one would decrease the separation of two molecules. a. collection of large elution fractions b. very slow flow rate of mobile phase c. too weak of an adsorbent 3. When 2-propanol was used as the developing solvent, two substances moved with the solvent front (R₁ = 1) during TLC analysis on a silica gel plate. Can you conclude that the two compounds are identical? If not, what additional TLC experiment(s) would you perform to investigate further?
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