What was the most important parameter change that lead to the development of modern HPLC?   A. Smaller particle sizes for the stationary phase   B. Ultra pure solvents for the detectors   C. Properly trained glass blowers to create the required glass tubes   D. High pressure pumps and tubing for the mobile phase Why do columns for gas chromatography need to be so long? Choose the best answer.   A. To overcome the problem of high diffusion due to the high temperatures   B. Because it is a thin glass tube   C. To prevent stationary phase loss and then contamination of the detector   D. To remove neutral contaminants from the background

Explain how Thin Layer Chromatography allows for the separation of the components of a mixture on a TLC plate? Please include these words: equilibrium, stationary and mobile phase, nonpolar and polar analytes, affinitity...which analytes (polar/nonpolar) have greater affinity for stationary or mobile phase?

What are the general characteristics of Gas Chromatography, Thin Layer Chromatography, Ion Exchange, HPLC, SEC and Affinity Chromatography?   What elutes first?   Additionally can you explain what exactly is stationary phase and mobile phase?

In Chromatography - Equilibrium Binding Isotherms, If diffusion and dispersion are negligible a) Explain why the effective velocity is a constant if the binding of the separand to the matrix follows a linear isotherm. b) In analytical chromatography, the band or elution peak is very narrow and has a symmetrical Gaussian shape. Explain why c) Explain qualitatively why the front end of the separand band always self-sharpens and the trailing end tends to spread out if the binding follows a Langmuir isotherm. d) What would happen to the elution peak/migration band if the column is "overloaded" (c > S).