1. What happens if sample for Potassium determination is refrigerated? What causes the variation? 2. What is the role/importance of Potassium in the body metabolism? 3. In doing the assay, why is hemolyzed sample not allowed?
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1. What happens if sample for Potassium determination is refrigerated? What causes the variation?
2. What is the role/importance of Potassium in the body
3. In doing the assay, why is hemolyzed sample not allowed?
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- 1. cite the differences between tube dilution and microtiter plate dilution. 2. why is it important to isolate the peripheral blood mononuclear cells completely from the other cellular components of the blood? 3. what laboratory assays can be performed to assess the efficiency of the sample preparation and isolation of the PBMC?1. Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test? 2. What level of Rheumatoid Factor in serum is clinically significant? 3. Describe how the Rheumatoid Factor concentration is computed.1. Why is it necessary to avoid underfilled blood samples in anticoagulant evacuated tubes? 2. Explain the purpose of thixotropic gel in a blood collection tube. 3. What is the rationale behind the sequence of the order of draw? a. Sterile tube (blood culture) b. Blue-top coagulation tube C. Serum tube (glass) with no additive d. Serum tube with clot activator, with or without е. Heparin tube with or without gel plasma separator f. EDTA tube g. Glycolytic inhibitor tube 4. What are the potentially affected tests if an evacuate tube with silica clot activator was filled first before a blue-top coagulation tube?
- 17. Which of the following situations below show an example of colored-top tubes interfering with the assay? O EDTA plasma used in compatibility testing, rather than serum Citrated tubes used to rule out platelet satellitism O EDTA plasma used in electrolyte determination Thrombin tubes used for the determination of potassium1. Discuss the principle of the test. ACTIVITY NO. 11: EUGLOBULIN CLOT LYSIS TIME (ECLT) 2. What are the physiologic and pathologic causes of deranged ECLT results? DECREASED ECLT INCREASED ECLT PHYSIOLOGIC PATHOLOGIC 3. List down 5 possible interfering factors of this test. 4. Illustrate and label the steps of this procedure.1. How will you differentiate VDRL from RPR Card test? 2. Give the composition of VDRL reagent and their function.
- 1. Differentiate the 2 specific types of VDRL namely: 1.1 quantitative VDRL 1.2 qualitative VDRL 2. Describe the principle behind the RPR (Rapid Plasma Reagin) Test. 3. What precautionary measures should be observed in the collection and preparation of specimen for VDRL examination?| 1 1 1 1 1 1. State the significance of compatibility test in transfusion medicine? 2. What is a major crossmatch? A minor crossmatch? 3. What incompatibilities are detected in the following phases? a. Protein phase b. Thermo phase c. AHG phase 4. Enumerate some transfusion reactions due to incompatible blood transfusion?3. Explain the purpose of thixotropic gel in a blood collection tube.
- 5. what is the most common indicattions for fecal occult blood testing are?1. Define and Describe aseptic technique in blood collection. 2. Explain the action of SPS as anticoagulant for blood culture.a. Silver reduction test: What is the substance responsible for the color production? b. Jaffe’s test: To what is the color due to? c. Ammoniacal zinc chloride test: What compound is responsible for the color? What are the pigments normally present in urine? d. Sulfates: From what substance are sulfates derived? In what conditions is sulfate elimination increased? e. Detection of Chlorides: What is the normal amount of chlorides eliminated in 24 hours? In what forms are they eliminated? In what conditions is the elimination diminished and eliminated increased? What is a threshold substance? Is chloride a substance substance? f. Detection of Phosphates: What is a triple phosphate? When it is formed? What are the sources of the phosphates found in urine?