1. The substrate concentration and reaction velocities for both an uninhibited and an inhibited reaction are listed in the table below. Use this data to produce a Lineweaver Burk graph. a. Use Excel or Sheets b. Label both axes and include units 2. Solve for km and Vmax for the uninhibited reaction only. a. Use equation editor to show your work 3. Graph both linear relationships on the same graph to determine the type of inhibition. a. Provide an explanation c. Include the trendlines and the corresponding equations d. Include the data for each trendline e. Use equation editor to show your work for the x intercept for both lines substrate (mm) v (mm/min), no inhibition v (mm/min), with inhibitor 100 11 156 18 222 27 323 40 1 2 4 8
1. The substrate concentration and reaction velocities for both an uninhibited and an inhibited reaction are listed in the table below. Use this data to produce a Lineweaver Burk graph. a. Use Excel or Sheets b. Label both axes and include units 2. Solve for km and Vmax for the uninhibited reaction only. a. Use equation editor to show your work 3. Graph both linear relationships on the same graph to determine the type of inhibition. a. Provide an explanation c. Include the trendlines and the corresponding equations d. Include the data for each trendline e. Use equation editor to show your work for the x intercept for both lines substrate (mm) v (mm/min), no inhibition v (mm/min), with inhibitor 100 11 156 18 222 27 323 40 1 2 4 8
Chapter30: Kinetic Methods Of Analysis
Section: Chapter Questions
Problem 30.12QAP
Related questions
Question
Transcribed Image Text:1. The substrate concentration and reaction velocities for both an uninhibited and an inhibited
reaction are listed in the table below. Use this data to produce a Lineweaver Burk graph.
a. Use Excel or Sheets
b. Label both axes and include units
2. Solve for km and Vmax for the uninhibited reaction only.
a. Use equation editor to show your work
3. Graph both linear relationships on the same graph to determine the type of inhibition.
a. Provide an explanation
c. Include the trendlines and the corresponding equations
d. Include the data for each trendline
e. Use equation editor to show your work for the x intercept for both lines
substrate (mm) v (mm/min), no inhibition v (mm/min), with inhibitor
100
11
156
18
222
27
323
40
1
2
4
8
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