1. Solution Amount pH W 0.1 14 X 0.1 2.9 Y 0.01 2 Z 0.001 3 Which solution is prepared with weak acids.?   2. Why does changes in pH and temperature affect the efficiency of enzymes? A. Protein is altered. B. Changes in surfase are not recognizable. C. Activation energy is lowered. D. Enzymes work best in normal temperature and pH.

What term is used to describe a species which affects the functioning of an allosteric enzyme?   a. regulator   b. controller   c. inhibitor   d. alloster

Enzyme X can act on either substrate A or B; the Km on A < Km on B. Vmax for A > Vmax on B.Sketch 1 plot for 1/v vs. 1/[S] plot for Enzyme X in the presence of A and in the presence of B.

Please answer all 8 blanks please!

Measuring food consumption and excretion A 22g field mouse consumed 5g of mouse chow that had an energy content of 2Cal/g in 24hours andduring that time produced urine and feces with an energy content of 6Cal.a. What was the amount of energy that the mouse used that day?b. What is the field mouse’ mass specific metabolic rate in J/g*hr?

4.     The amino acid Asp189 lies at the base of the substrate specificity pocket in the enzyme trypsin. a.   How is this related to typsin’s substrate specificity? Briefly describe the interactions between the substrate and the Asp 189. b.   If the Asp189 was replaced with a lysine residue, how would this affect substrate specificity? c.   The scientists that actually mutated the Asp189 to a lysine analyzed the three-dimensional structure of the enzyme and found that the lysine is actually not located in the specificity pocket. Instead, the Lys side chain reaches out of the base of the pocket, rendering the pocket nonpolar. With this additional information, determine how the substrate specificity would differ in the lysine-mutated enzyme.

Which statement(s) is(are) true about carbonic anhydrase enzyme?A. Carbonic anhydrase enzyme controls the production of carbonic acid.B. Carbonic anhydrase enzyme controls the pH of human blood.C. Zn metal is present in the catalytic site.

2. What class of enzyme would expect to catalyze the following reaction? H,NCHCNHCHCO H,NCHCOH H,0 R R a. Oxidoreductase b. Transferase c. Hydrolase d. Lyase e. Isomerase

13. A specific enzyme will usually only catalyze one particular reacton. True  False

Most living systems that we know about function best in the temperature range of 20-40 C. Knowing this, at what temperature do you think the enzyme will function optimally?