1. List as many as possible blood bank serologic tests that are affected by hemolyzed samples (list as many as possible and how) 2. Enumerate and describe the sources of antibodies utilized in the determination of blood group soluble substances.
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1. List as many as possible blood bank serologic tests that are affected by hemolyzed samples (list as many as possible and how)
2. Enumerate and describe the sources of antibodies utilized in the determination of blood group soluble substances.
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- VIII. Blood Types Fill in the blanks to complete each of the following sentences. Choose from the words listed in the Word Bank. AGGLUTINATION HEMOLYSIS POSITIVE ANTIBODIES NEGATIVE RED BLOOD CELLS ANTIGEN PLASMA A AB ,of which there are two types (A 1. Each red blood cell carries a protein called an and B). 2. Plasma carries 3. A transfusion reaction occurs when the antibodies of one blood type attack the cells of the other blood type, causing the cells to clump together; the process of producing large clumps of cells is called 4. During a reaction, red blood cells may burst; this is called 5. Blood type completely accurate. Reactions usually do not occur because only the transfused. against the of the other blood types. is sometimes called the universal donor, although the term is not are being is sometimes called the universal recipient, although this is not 6. Blood type completely accurate either. 7. Many people also carry another antigen, called the Rh antigen; when this is the…1. What is the principle involved in Hemagglutination (HA) and Hemagglutination Inhibition Tests (HAI). 2. What happens to an erythrocyte during an indirect or passive hemagglutination technique? 3. Identify possible sources of erythrocytes that may function as carrier for detecting and titrating the corresponding antibodies during hemagglutination.1. What is an antiserum? 2. What are the potency requirements in an antiserum? 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-B
- 1. Give the advantages of using tube method over the slide method. Why do you have to incubate the tube method in Rh typing? 2. Give the clinical importance of determining the Rh status of a patient? 3. Define HDN and explain the effect of Rh(D) antigen. What is the preventive measure of preventing D-negative mothers from forming anti-D? 4. What are the symptoms in Rh-mediated hemolytic transfusion reactions?7: Antibody titer is the reciprocal of the highest dilution of the patient’s serum in which the antibody is still detectable (gives a positive reaction with the antigen) * False True1. What is the purpose of Rh view bow? What is the optimum temperature for detection of Rh antigen? 2. Give the different types of Rh antigen typing reagents accdg. to the sources. Explain each.
- state the most common reason for a false-negative antibody test1.2 Mr Juju verbalises that he was vaccinated for corona viruses two weeks ago 1.2.1. Explain the role of antibodies following vaccination 1.3 A week later: Mr Juju presents with Blood Glucose of 17.8mm/dl and blood pressure of 90/50mmHg 1.3.1. Describe the action insulin in response to elevated blood glucose to maintain blood glucose to normal ranges 1.3.2. Explain the influence of hormonal regulation to maintain normal blood pressure under the following: 1.3.2.1. Renin-angiotensin-aldosterone system (RAAS) 1.3.2.2. Atrial natriuretic peptide (ANP)1. What is an antiserum? 2. What are the potency requirements in an antiserum? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.4 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-B
- 1. What is/are the importance of testing Rh blood type in the laboratory? 2. Why is Rh system determination essential to pregnant and childbearing women? 3. What are the principal Rh antigens?&9. Explain the principle and step by step procedure of Dry Blood Spots collection.?1. If the MCHC value is decreased, what term is used to describe the peripheral blood smear finding? 2. Aside from automated and microhematocrit determinations, what red blood cell index can be used for indirect measurement of hematocrit? 3. Automated and microhematocrit determinations present discrepancy in the results due to what factor? 4. What is the wavelength used in the hemiglobincyanide method of hemoglobin determination? (Answer in this format: numerical value space abbreviated unit)