1. Initial rates of enzyme-catalyzed reaction for various substrate concentrations are listed below: Rate Substrate concentration mol/(L min)x10 177 (mol/L) 0.0041 173 125 0.00095 0.00052 106 0.000103 80 0.000049 67 0.0000106 43 0.0000051 a) Evaluate Vmax and Km by a Lineweaver-Burk plot.

1. Initial rates of enzyme-catalyzed reaction for various substrate concentrations are listed below: Rate Substrate concentration mol/(L min)x10 177 (mol/L) 0.0041 173 125 0.00095 0.00052 106 0.000103 80 0.000049 67 0.0000106 43 0.0000051 a) Evaluate Vmax and Km by a Lineweaver-Burk plot.

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter11: Chemical Kinetics: Rates Of Reactions

Section: Chapter Questions

Problem 7QRT

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Question

Problems on enzyme reaction kinetics
1. Initial rates of enzyme-catalyzed reaction for various substrate concentrations are listed
below:
Rate
Substrate concentration
mol/(L min)x10
177
(mol/L)
0.0041
173
0.00095
0.00052
125
106
0.000103
80
0.000049
0.0000106
0.0000051
67
43
a)
Evaluate Vmax and Km by a Lineweaver-Burk plot.

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