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- 1. . The obligate anaerobic bacterium Clostridium perfringens dies when exposed to air. What would predict as the result if you performed a catalase test on this bacterial species? Explain why.Would an obligate anaerobe or an obligate aerobe be more likely to produce catalase? Why?1. Escherichia coli but not Pyrolobus fumarii will grow at 40°C, while P. fumarii but not E. coli will grow at 110°C. What is happening (or not happening) to prevent growth of each organism at the non-permissive temperature?
- 9. Which metabolic pathways are used by an obligate aerobe, an obligate anaerobe, a facultative anaerobe?1. Clostridium butyricum does not appear to have superoxide dismutase (SOD) and catalase enzymes. How would the growth of C. butyricum in oxic conditions compare to its growth in anoxic conditions? On this basis, what is the aerotolerance category of C. butyricum? 2. Citrobacter freundii is a facultatively anaerobic bacterium. Why would you expect this bacterium to grow better (i.e. form larger colonies) in an oxic environment than in an anoxic one? (as a hint, do some research on the “Pasteur effect”)5)A certain aerobic organism is able to metabolize the followingglycolipid CH,OH H H OH HO OH
- Catalase combines two hydrogen peroxide molecules (H2O2+H2O2) to make two molecules of water. A gas also forms. What is the gas?53. Which one of the following bacteria group CANNOT survive in presence of oxygen? Group of answer choices obligate aerobes obligate fermenters facultative aerobes anaerobes microaerophiles aerotolerant 54. Identify the MISMATCH pair from the following, Group of answer choices E.coli :::: facultative aerobe group Bacteroides :::: obligate anaerobes none of the above is a mismatched pair Chlamydia sps :::: cell culture for cultivation Gardnerella sps :::: bacterial vaginosis Staphylococcus sps :::: can tolerate 20 % salt; cause pimples Rickettsias :::: obligate intracellular parasite1.What is the purpose of resazurin in thioglycollate medium? 2.Why does thioglycollate medium have only a small amount of agar in it? 3.How is a facultative anaerobe different from a facultative halophile? 4.Explain how a strict aerobe is different from a strict anaerobe. 5.What would happen to the bacteria if you accidentally closed all of the caps all the way? Would any of the cultures have grown? If so, explain which would grow and which wouldn’t.
- 1. Define the following terms: a. Chermolithoheterotroph b. Microaerophile c. Chermoorganoheterotroph d. Obligate anaerobe e. Facultative anaerobic f. Coliform6. If a facultative anaerobe was in an oxygenated environment, which metabolic pathways would it use? Why?Where in the human body would you expect to find an obligate aerobe? Where would you expect to find an obligate anaerobe? Look up some examples of each and indicate where they may be found inthe body.
