1 View and DRAW observations of prepared slides: Bacteria Special Stains a. Endospore Stain: Clostridium tetani and Clostridium botulinum-Label: endospores, note location of endospore for each species b.) Flagella Stain: Proteus vulgaris and Spirillum-MY- volutans-Label: flagella, identify flagella arrangement on each type of organism. P

Give an example of a type of clinically relevant bacteria that are spore formers. For example what are the spore formers that cause diseases in humans?

Culture Code COLONY MORPHOLOGY GRAM STAIN Name the Bacterium   Color Form Surface Opacity Cell color Cell shape Cell Grouping  G+ or G-                       A                                       B                                       C                                       D                                       E

Bonus: You are treating a patient with inhalation anthrax with antibiotics. After 2 weeks of antibiotics, the patient seems greatly improved and you discharge the patient. However in 1 week, the patient returns, obviously still suffering with inhalation anthrax. If the antibiotic you chose was based on antibiotic sensitivity tests, what would be the best explanation for the lack of treatment success? Bacillus anthracis_makes endospores which are resistant to all antibiotics O the outer membrane of the pathogen cell wall inhibited diffusion of the antibiotic into the cll O the antibioitc you chose could not cross the cell wall of the pathogen O the pathogen has a thick layer of peptidoglycan which prevent diffusion of the antibiotic into the cell Ouestion 10

Identify the cellular morphology for the above Gram stain. What is the Gram reaction? What is the bacterial shape? What is the bacterial arrangement? J [Choose ] [Choose ] Singles / no arrangement Bacilli Chains Gram-negative Grapelike clusters Gram-positive Spirilla Cocci

Why are staining methods used in microbiology? Choose all that apply A. Staining results can have medical implications for an infected B. To clearly see the internal structures of the bacteria C. The stains can be used to dinginuish between bacteria type D. It is easier to very small microscope.

Identify the cellular morphology for this Gram stain. What is the Gram reaction? What is the bacterial shape? What is the bacterial arrangement? What is the staining dye? [Choose ] [Choose ] Cocci Singles no arrangement Pleomorphic Chains Gram-negative Crystal Violet Phenol Red Bacilli Gram-positive Clusters Safranin

Examine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information A. Shape ........; Gram stain ( Gram (+), Gram (-) or both ... B. Stained color under microscope..... . C. Is the PG wall think, thin or both ..

What bacterial structure is present in the image below? Flagella Capsule -L Glycocalyx Endospore y

Pls can you identify this organism The first picture is gram stain negative The second pictures is endospore stain