Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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1) A forensic scientist is required to collect blood samples from the crime scene and
analyze it to help the investigators understand who participated to the murder.
i) As a first step, the scientist needs to extract DNA. Indicate why red blood cells are not a
good target for the analysis, and list at least two chemicals/compounds that are required to
extract the DNA, explaining why they are used.
ii) The sample has been collected in a small amount, so that (s)he decides to use PCR to
analyze it. Describe why PCR is better suited to address small samples with respect to
Southern blotting and how is selectivity addressed with this technique.
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Transcribed Image Text:1) A forensic scientist is required to collect blood samples from the crime scene and analyze it to help the investigators understand who participated to the murder. i) As a first step, the scientist needs to extract DNA. Indicate why red blood cells are not a good target for the analysis, and list at least two chemicals/compounds that are required to extract the DNA, explaining why they are used. ii) The sample has been collected in a small amount, so that (s)he decides to use PCR to analyze it. Describe why PCR is better suited to address small samples with respect to Southern blotting and how is selectivity addressed with this technique.
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