Aim: To investigate the vitamin C content of fruit juice. Introduction In this experiment I will be finding out which fruit juice contains the most vitamin C. I will be using a method similar to titration but I will be using a syringe instead of a burette.
Vitamin C Vitamin C also known as ascorbic acid is needed for growth and maintenance of healthy tissues, especially skin, blood vessels, bones, gums, teeth. Vitamin C aids in resistance against infection and healing of wounds. It also helps the body absorb iron from food. Vitamin C can be found in green vegetables, potatoes, tomatoes and citrus fruits such as oranges and lemon. A lack of vitamin C can cause scurvy, iron deficiency and poor wound healing. A healthy diet
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My dependant variable is the amount of Vitamin C in each fruit juice. I will observe the solution when it is decolourizing. I will use the same volume of DCPIP solution for each fruit juice so that the results are accurate and valid. I will use the results from this experiment to work out how much vitamin C the fruit juices contain.
Preliminary Test In the preliminary test I used 3cm3 of DCPIP solution. A large amount of juice was needed to change the colour of the DCPIP to colourless. I changed the amount of DCPIP solution from 3cm3 to 1cm3. This is because less juice will be needed to change 1cm3 of DCPIP from blue to colourless. I used test tubes to hold the DCPIP and the 1% ascorbic acid but it was difficult to mix the two solutions together so I decided to use a boiling tube instead. I tested the 1% ascorbic acid in the preliminary test and I found that I need less than 3cm3 of the solution.
Equipment
• 12x Boiling tube
• 2x Test tube rack
• 1% DCPIP solution (1cm3 x15)
• 4x 5ml syringe
• 1% ascorbic acid (3cm3 x3)
• Pineapple juice (30cm3 x3)
• Orange Juice (30cm3 x3)
• Lemon juice (30cm3 x3)
The boiling tubes will be used to hold the juice and the 1 % DCPIP solution. I will use boiling tubes because in the preliminary test I used a test tube and I found that the test tube was too small and was hard to shake the mixture therefore I will be using boiling tubes as the are slightly
In a third test tube, put 1 mL of chutney. Repeat steps 2 and 3 with the chutney and record the results in Table 3.
First students obtained 8 graduated cylinders and labeled them. Each one contained a different ratio of vinegar and water. These graduated cylinders with the liquid were weighed and recorded.Then, students obtained an Alka-Seltzer tablet and recorded its mass. Then one alka-seltzer tablet was dropped into each of the graduated cylinders. Students had to
8 test tubes were then labelled A-H, 1ml of DCPIP was added to each of the test tubes. 2ml of NaHCO3 was added tubes A through G.
My partner and I choose glucose, colchicine, cold temperature (0 degrees Celsius), and warm temperature (37 degrees Celsius) as the addition factors. The tetrahymena were exposed exposed to their condition for ten minutes before the India ink was placed in their microcentrifuge. The tertrahymena were fixed on a slide every ten minutes after the ink was placed in the microcentrifuge. In this experiment there are two controls. There is positive control and a negative control. The untreated sample is tetrahymena that did not have any other conditions besides the ink. Then before the sample was placed on the slide they were fixed. The treated sample was tetrahymen mixed with
The purpose of this experiment was to alter the cell membrane of the beets, in a given fashion, so that we can test how much betacyanin was able to cross the cell membrane of the beet through various treatment. In which after we would test the absorbance level of each treatment, run it through excel and observe which treatment was most effective at getting betacyanin through the cell membrane more.
The first step that needed to be done in this experiment was adding hydrochloric acid (HCl)
XII. Take the 250 ml beaker to your lab bench. Set up a gravity filtration with a plastic funnel, folded wet filter paper, and an Erlenmeyer flask. Pour the content in the 250 ml beaker slowly through the filter paper. Wash the filter paper with deionized water. Dispose of the filtrate in the proper labeled waste container.
Higher levels of solution should produce higher levels of product. The independent variable for the control group data and the experiment data is the yeast concentration. The dependent variable for the control group data and the experiment data is how much oxygen is produced. The Constant for the control group data and the experiment data is time and amount of hydrogen peroxide. The products of the experiment will increase if the levels of reactants increase. Denatured yeast may cause change in the reaction of the experiment. For all trials of the control group, the concentration of yeast is 6ml. For the experiment data, the yeast concentration varies from 8mL, 10 mL, 12 mL, 14 mL, and 16 mL. The temperature may cause change in the reaction of the combination of yeast and hydrogen peroxide
Repeat steps 4–11 two more times. Be sure to record your observations in the data tables in your lab notebooks, in the rows for trials 2 and 3. (Can you separate the dyes of grape soda using space sand?). The entirety of the Procedures.
The purpose of the experiment was to find a solution which would prevent an apple from turning brown, and to figure out whether the pH of a solution has an effect on the browning of cut apples. My lab partners and I thought that if we dipped the apples in lemon juice then it would prevent the apples from browning compared to other solutions. Solution A had a pH of 4, solution B had a pH 3, solution C had pH of 4, Solution D had a pH of 4. All the solutions apples including the no solution apples had a browning scale of 0 at the 0 minute mark. After 24 hours of soaking solution A had an average browning of 3, solution had B had an average browning of 0, solution C had an average browning of 3, solutions D had an average of 1.7, and the no solution
Vitamin C is crucial for survival for several reasons. For a human body to reach the acme of its physical potential, it must have a daily intake of vitamin C. Deficiency can lead to a fatal symptom known as scurvy. Likewise, iron absorption will be more difficult resulting in iron-deficiency anemia (Smith, Primio & Humphrey-Murto, 2011). However, this nutrient is essential for bodily functions as well. Vitamin C is required for hormone production which is seen by the fact that the highest concentration of it in the human body is in the pituitary and adrenal glands (Emsley, 2004). Moreover, this vitamin aids in protection against free radicals and stress. Vitamin C is also imperative because it assists in the growth of various body parts, including teeth, cartilage, and dentine. This acid is integral for bone development and to heal fractured or broken bones. As said before, “Vitamin C is important in the absorption and metabolism of many nutrients that affect production of red blood cells”, including iron absorption (Smith, Primio, & Humphrey-Murto, 2011). DIRECT QUOTE WITHOUT AUTHOR. Similar to its importance to humans, vitamin C is vital for plant life as well. Plants require the alcohol for growth processes.
In the experiment we used Turnip, Hydrogen Peroxide, Distilled Water, and Guaiacol as my substances. On the first activity, Effect of Enzyme concentration of Reaction Rate for low enzyme concentration, we tested three concentrations of the turnip extract, and hydrogen peroxide. For the Turnip Extract I used 0.5 ml, 1.0 ml, and 2.0 ml. For hydrogen peroxide we used 0.1 ml, 0.2 ml, and 0.4 ml. We used a control to see the standard, and used a control for each enzyme concentration used. The control contains turnip extract and the color reagent, Guaiacol. We prepared my substrate tubes separately from the enzyme tubes. My substrate tube
Orange Juice Materials: Three oranges were used for this experiment to obtain a decent amount of juice. Each orange was cut in half and juiced according to steps 1 and 2 of Appendix I of the Laboratory Manual for Biology 201 (Denniston, Wimmers, & Hemm 2015). The juice from the first orange and one half of the second orange was strained into a small beaker following
Vitamin C is an organic compound that is also known as ascorbic acid. The molecular formula for Vitamin C is C6O8H6. This structure contains a 5-membered ring. Vitamin C contains 4 alcohol functional groups, an alkene, and an ester. Within the ring it contains one double bond, the carbon=carbon bond in the ring structure, this is an alkene functional group. The Vitamin C structure also contains an ester which helps form the five-membered ring.
Then, each group of students received the necessary materials to complete the experiment. When the students received the cups, they labeled cups to distinguish between the salt solution, distilled water, and control group. After weighing the cups and finding the mass of the cucumbers, the students poured 50 ml of water in one cup, 50 ml of salt solution in the other, and left the control cup empty. Then, the students placed the cucumbers into the cups and waited 30 minutes for the results. After the 30 minutes, the students removed the cucumbers from each solution and dried the cucumbers with paper towels. The students then weighed the cucumbers again and recorded their results. Lastly, the students found the difference from the original mass of the cucumbers and recorded their results.