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Vitamin C Content in Fruit Juices

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Aim: To investigate the vitamin C content of fruit juice. Introduction In this experiment I will be finding out which fruit juice contains the most vitamin C. I will be using a method similar to titration but I will be using a syringe instead of a burette.

Vitamin C Vitamin C also known as ascorbic acid is needed for growth and maintenance of healthy tissues, especially skin, blood vessels, bones, gums, teeth. Vitamin C aids in resistance against infection and healing of wounds. It also helps the body absorb iron from food. Vitamin C can be found in green vegetables, potatoes, tomatoes and citrus fruits such as oranges and lemon. A lack of vitamin C can cause scurvy, iron deficiency and poor wound healing. A healthy diet …show more content…

My dependant variable is the amount of Vitamin C in each fruit juice. I will observe the solution when it is decolourizing. I will use the same volume of DCPIP solution for each fruit juice so that the results are accurate and valid. I will use the results from this experiment to work out how much vitamin C the fruit juices contain.

Preliminary Test In the preliminary test I used 3cm3 of DCPIP solution. A large amount of juice was needed to change the colour of the DCPIP to colourless. I changed the amount of DCPIP solution from 3cm3 to 1cm3. This is because less juice will be needed to change 1cm3 of DCPIP from blue to colourless. I used test tubes to hold the DCPIP and the 1% ascorbic acid but it was difficult to mix the two solutions together so I decided to use a boiling tube instead. I tested the 1% ascorbic acid in the preliminary test and I found that I need less than 3cm3 of the solution.

Equipment
• 12x Boiling tube
• 2x Test tube rack
• 1% DCPIP solution (1cm3 x15)
• 4x 5ml syringe
• 1% ascorbic acid (3cm3 x3)
• Pineapple juice (30cm3 x3)
• Orange Juice (30cm3 x3)
• Lemon juice (30cm3 x3)

The boiling tubes will be used to hold the juice and the 1 % DCPIP solution. I will use boiling tubes because in the preliminary test I used a test tube and I found that the test tube was too small and was hard to shake the mixture therefore I will be using boiling tubes as the are slightly

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