The Growth Of Bacteria Known As Citrobacter Freundii As Well As Distinguishing Antibiotics Effects Its Growth

The aim of this experiment was to observe the effect different concentrations of Dettol had on the growth of S. albus. The highest concentration of Dettol (100% - D) was predicted to be the most effective solution to kill and stop the growth of this particular bacteria. This can be justified as S. albus is gram-positive; easier bacteria to kill, developing resistance slower than a gram-negative. Therefore, the highest concentration of antibiotic should be – and was – the most successful in changing the pH levels, and killing S. albus. Through discussion and analysis of the results collected through the experiment, it can be concluded that there is higher chance of killing and the growth of this particular bacteria if 100% Dettol is used.

Effect of 5-Fluorouracil, Penicillin G and Amphotericin on the growth of Pythium and Micrococcus luteus

The purpose of this study was to identify the unknown bacterium #305. This sample was collected from the urine of a 64 year old hospital patient named “Doris”. Several tests were conducted to determine the morphological and physiological characteristics of the unknown. These tests included but were not limited to: motility, gram staining, salinity tolerance, and fermentation of different substances. After assessing the observed results, the identity of unknown bacterium #305 was suggested to be Citrobacter freundii. The test results, with the exception of the urea test, were very consistent with the expected results.

Our world has changed dramatically since the day Antoine van Leeuwenhoek discovered microorganisms in 1676 using a simple microscope. In early days, scientists first thought life arose from inanimate materials. This theory, known as abiogenesis or spontaneous generation, was disproved later on by scientists including Lazarro Spallanzani and Louis Pasteur. The experiments conducted by these scientists showed that living things could only arise from preexisting life, or biogenesis. All life begins with a living cell, composing of five required components. These components are DNA, RNA, cell membrane, ribosome, and cytoplasm. As more investigations on bacteria were conducted, scientists were able to acquire a deeper knowledge of the microbiology and pathology of animals, plants, and humans.

Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).

The antibiotics will have different zones of inhibition on different bacteria. Also, a range of concentrations of the nutrients in the bacteria will change the bacterial growth.

The aim of this investigation was to find out which antiseptics were most effective at preventing the growth of bacteria.

Throughout my life, adults have insisted the use of antibiotics to fight against the most inconsequential illnesses, whether it’s the cold or the flu. However, neither illness is due to invasion of bacteria. This misuse can lead to antibiotic resistance, also known as antimicrobial resistance(AMR), currently one of the central issues facing the public health system. While the process for antibiotic resistance occurs naturally through the process of adaptation, the mismanagement of antibiotic resources has accelerated the rate at which the bacteria adapt. The occurrence of this misinformation isn’t limited to a few adults: even some of my peers suggest taking antibiotics when faced with the flu. This leads to asking whether AMR is truly a problem and are present regulations enough to combat the issue.

Being able to control bacterial growth is something that is important in our everyday lives. As shown in the previous labs, bacteria can grow and create colonies extremely quickly especially in the right environments. By acknowledging this, it is then important to get an understanding of how bacterial growth can be controlled by humans. To control microorganisms it means to inhibit their growth (static) and or kill them (cidal) (Kenneth Todar, 2015); therefore since focusing on bacteria the terms bactericidal and bacteriostatic are both extremely important for this lab. One broad method we will use to control bacterial growth is heat. The amount of heat needed to control bacterial growth is different for different species of bacteria (Kenneth Todar, 2015). Bacteria can also respond differently depending if moist heating method such as an autoclave with steam is used, or a dry heating method such as inoculating a loop over a fire is used (Kenneth Todar, 2015). UV works by damaging the cells DNA, without proper DNA, the cells will die and the object

A potential outbreak was first reported on September 15 vie a telephone call at 6:00 am. At first, there was only a possibility that it was an outbreak when it was reported that 30 individuals were home sick, when usually it was 4 out of 200 employees. It was not until after interviewing five of the sick individuals over the phone that there was little doubt that it was an outbreak, as their only recent interactions between themselves were during the company’s dinner at Lou’s Center City Restaurant on the 14th at 6:00 pm, shows similar symptoms of cramps and diarrhea, and became sick between 2 am and 8 am the next day. The interviews were completed between 9:00 am to 10:00 am.

The introduction of different antimicrobials for treating different infections exhibited the importance of having antimicrobial susceptibility testing as a repetitive method in all microbiology laboratories. In laboratories this can be possible by using antibiotic disk that will diffuse gradually into the medium where the suspected organism is located. The fundamental principle of the antibiotic susceptibility testing was already being used in microbiology laboratories over 80 years. Several chemical agents like for example antiseptics, disinfectants, and antibiotics are engaged to fight the growth of microbes. Among these, antibiotics are commonly defined as the substances that microorganisms produce like Penicillium that can kill or inhibit the growth of other microorganisms, primarily bacteria. Antimicrobial susceptibility tests (ASTs) principally measures the capacity of an antibiotic or other antimicrobial agent to prevent microbial growth in vivo.

Results indicated that all tested strains could grow with streptomycin at 55oC with different growth efficiencies. Six strains had high growth, 8 showed medium growth and 18 had low growth efficiencies. Comparing the growth of the same strains (Tables 11 and 12) indicated different growth efficiencies within some strains where some strains showed less efficiencies in Table 12 than their behavior in Table 11, i.e., strains codes 16, 20 and 58, other strains showed higher growth in Table 12 than their performance in Table 11, i.e., strains codes 33, 43, 45, 52, 61, 62, 66, 70, 73 and 84. These results indicated that some fusants are still instable.

The purpose of this lab is to observe bacterial growth between a water fountain and door handle

This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density

The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment