Introduction -
Background -
PTC stands for Phenylthiocarbamide, a chemical that can either taste very bitter, or nothing at all depending on the genetic makeup of the subject. People who can taste this are supertasters. Supertasters are people who are more sensitive to taste than others, this is linked to the tas2r38 gene. Another link to supertasting is fungiform papillae density. Fungiform papillae are club shaped parts on the tongue that a slightly raised over the rest of the tongue; they have taste buds that can distinguish between the five tastes sweet, sour, bitter, salty, and umami. Linda Bartoshuk stumbled upon supertasting at Yale studing saccrain. She found people reacted to saccrain differently, some found it sweet, but some tasted a bitter flavor along with it. After extensive
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Hypothesis - Factors that increase evaporation will increase transpiration.
Prediction - Light and wind will have the highest rate of transpiration.
Methods -
The method we used was the same as the one conducted in experiment 15 Super Taster. (Leady, 2018) No changes were made to the procedure. First we obtained a kit to use. Next we would dry the tongue and apply the blue food coloring using a cotton swab. Lastly we would take a picture of the cardstock over the tongue to count to fungiform papillae. We also tasted whether or not we tasted the PTC bitterness discreetly trying to not let the partner know my reaction.
Results - For our results we got results that somewhat correlated to what we expected. People who were in our class did show an increased amount of fungiform papillae density. This is because people who were able to taste the PTC had on average more fungiform papillae. Supertasters had an extreme amount of papillae at the largest density of the three groups and as predicted the PTC non-tasters correlated with a lower amount of fungiform papillae.
Discussion / Conclusion -
PTC non-taster
“Phenylthiocarbamide (PTC) and its chemically related compound, 6-n-propylthiouracil (PROP), provide extremely bitter taste to some subjects (tasters) but are tasteless or only slightly bitter to others (nontasters)” (Hong, et al.,711-15). There have been many experiments done to examine the relationship between PTC tasting and food preferences. “Although PTC itself has not been found in nature, the ability to taste PTC is correlated strongly with the ability to taste other naturally occurring bitter substances, many of which are toxic. Furthermore, variation in PTC taste sensitivity has been correlated with dietary preferences that may have significant health effects” (Wooding, et al., 637-646). Therefore, differences in PTC tasting can correspond with sensitivity to diseases that are related to diets in the human population. “The ability to taste the bitter compounds PTC and PROP was found to be a protective factor against cigarette smoking. On the other hand, PTC tasters may perceive vegetables such as cauliflower, cabbage, broccoli, and Brussels sprouts as unpleasantly bitter” (Floriano, et al., 931-941). Based on the information above one can conclude that PTC tasters are less susceptible to smoking cigarettes but they are also less likely to consume food that contain beneficial nutrients. A crucial point to understand is that although cruciferous
On October 12 2017, the class was set to collect DNA samples from a strawberry. Placing the strawberry in a ziplock bag and zip up the bag. Squish the strawberry into a liquid. Add 10 ml of the buffer (salty, soapy water) into a 10ml graduated cylinder using a transfer pipettes. Using the 10ml graduated cylinder, open up the ziplock bag and pour the 10 ml buffer with the squished up strawberry then zip up the ziplock bag. Mix it by moving it around using your fingers, but be cautious about how much bubbles are created. Add the a funnel lined with a cheesecloth onto a test tube (filtration apparatus) and filter the new berry-buffer solution in the filtering apparatus into a test tube. When finished with adding the new berry-buffer solution
The genotype - - in the PV92 locus could indeed have a positive affect and favored over ++ or +-. If an individual has the + allele they at least, have one ALu site. This site is a location where many mutations can appear and cause diseases or other defects (Lev-Maor,2003). For the TAS2R38 loci, being a test could have an advantage compared to non-tasters. The taste of PTC is a bitter sour taste like many acids and chemicals. This could help the survival of individuals due to their ability to taste this chemical; it could aid in protect from other bitter taste that may be harmful (Wooding,
L.H Snyder states that if PTC bitterness taste is inherited, if it would be safe to for it to be referred as a Mendelian
of the TAS2R receptor on the tongue situated in chromosome 7 denotes the capacity to taste PTC (Kim & Drayna, 2004). The most popular well-known strategies to taste PTC is a piece of paper that it is place on people 's tongue, and individuals who can 't taste the bitterness of the PTC paper are called "non-tasters", while the individuals who can are called "tasters"(Lawless, H. 1980).Through various studies it has been found that 15%-30% of individuals are non-tasters of PTC; however it has likewise been found that age, race, and sex assume a major part in who tastes and don 't taste PTC (Lawless, H. 1980). Sexual orientation has had an extremely intriguing relationship between PTC taster and non-tasters as it has been seen that women are more likely to PTC than men (Merritt, R, etc., 2008).
The experiment was conducted to test the taste and perception of males and females. It was predicted that if you are a male then you will test lower on the bitter scale. It was also predicted that if you taste the Sodium Benzoate as bitter then you will rate things higher on the bitter scale. A group of six formed and recorded their perception of different test strips and substances. A bitter-sweet scale was used to test how the participants judged their taste perception.
Every individual has a different response to everything one eats, drinks, sniffs or injects and something interesting is bound to happen.
Moreover, the p-value of the ANOVA results was well below 0.05 at p < 0.000001, revealing that the experiment’s results were significant, and a post hoc test of pairwise comparisons determined which yeast strain levels are significantly different from one another. The yeast strains levels of N50 and N25 were significantly
In previous studies, Frank and Byram’s article suggest that taste and smell interactions are dependent on taste and odor. In their experiments, they gave subjects strawberry
The expression of phenylthiocarbamide is very questionable concept given such a diverse and vast population of people living on earth with only a 0.1 of difference genetic. Phenylthiocarbamide is white fine powder that when ingest an individual can taste bitterness or nothing. In the mouth, there is gene called TAS2R that encodes for bitter taste receptor. The experiment will test the expression of TAS2R gene using phenylthiocarbamide or PTC on a selected population of Florida International University students for the recognition of bitterness or absent. Twenty-four undergraduate students of Florida International University will be participating in the experiment where height, age, sex, and ethnicity is negligible.
“Taste, like smell, is a doorman for the digestive tract, a chemical scan for the possible dangerous (bitter, sour) elements and desireable (salty, sweet) nutrients.” (46)
Certain scents were found to enhance the sweetness of solutions while others suppressed it. The point to which a scent smelled sweet was the best predictor of the taste scores. The sweetest smelling scent, caramel, was found to overpower the sourness of citric acid and to heighten the sweetness of sucrose. Scents with low sweetness inhibited the sweetness of the
Due to this information I made an educated guess and stated in my hypothesis that I believed a greater portion of the individuals I tested would be non-tasters. The data I collected, however, disproves my original hypothesis. A total 66.7% of the individuals I tested proved to be tasters, while the remaining 33.3% indicated no ability to taste the PTC. The results show that although the scale of non-tasters
Traditionally, experimental analysis of Homo sapiens taste thresholds has similarly focused on these two classes of biomolecules, with similar sweetness thresholds as those exhibited by members of the taxa Insecta being established in H. sapiens with respect to simple carbohydrates. For example, while Scheiner et. al. in (2003) (as quoted in Scheiner et al. 2004), demonstrated that a sucrose concentration above 3% was sufficient to induce a positive sweetness detection rate of close to 60% in a population of the honey bee, Apis mellifera, Richard Bowen (2016) has indicated the H. sapiens population response threshold resides around sucrose concentrations of 3.4%.
The process of tasting is happy for me, I enjoy the feeling that food melt in my mouse and the flavour disperse gradually. But it also exists some problems, I have the keen taste sense and smell over the average,