On October 12 2017, the class was set to collect DNA samples from a strawberry. Placing the strawberry in a ziplock bag and zip up the bag. Squish the strawberry into a liquid. Add 10 ml of the buffer (salty, soapy water) into a 10ml graduated cylinder using a transfer pipettes. Using the 10ml graduated cylinder, open up the ziplock bag and pour the 10 ml buffer with the squished up strawberry then zip up the ziplock bag. Mix it by moving it around using your fingers, but be cautious about how much bubbles are created. Add the a funnel lined with a cheesecloth onto a test tube (filtration apparatus) and filter the new berry-buffer solution in the filtering apparatus into a test tube. When finished with adding the new berry-buffer solution
In a third test tube, put 1 mL of chutney. Repeat steps 2 and 3 with the chutney and record the results in Table 3.
For the second part of the experiment I cut the cheesecloth into four pieces and folded them so that it was four layers thick. I placed one piece of cheesecloth into the funnel and measured 60mL of soil using the 100mL to help measure the soil and poured that into the funnel. Taking beaker number one I poured the contents into the funnel and let that filter into beaker number five. I used the same technique as above and I repeated the same thing to beakers number two through four and poured them into beakers number six through eight. Once this was done I observed beakers five through eight and wrote down my observations in Table 1.
The purpose of this lab was to determine if lima beans would be able to grow nice and big in gatorade. The hypothesis stated, if we put the lima bean in the gatorade then it will grow really big, fast because gatorade contains of electrolytes that might affect the lima beans growth. The hypothesis turned out to be disapproved because the lima beans average length in the water/ control starting from day 1 was 17.5 mm, 25.4 mm, 42mm, and 44mm. The lima beans average length in the gatorade was 17mm, 22.6mm, and by day 3, the lima beans grew mold. In addition to that, The total mass of the lima bean in the control was greater than the bean in the variable. The lima bean in the control’s total mass was 5.25g, 9g, 11.9g, and 20.5g. The lima bean
And finally into test tube 3, I pipetted 1.0 ml turnip extract and 4.0 ml of water. The contents of test tube 1 was poured into a spectrometer tube and labeled it “B” for blank. “B” tube was now inserted it into the spectrometer. An adjustment to the control knob was made to zero the absorbance reading on the spectrometer since one cannot continue the experiment if the spectrometer is not zeroed. A combination of two people and a stop watch was now needed to not only record the time of the reaction, but to mix the reagents in a precise and accurate manner. As my partner recorded the time, I quickly poured tube 3 into tube 2. I then poured tube 2 into the experiment spectrometer tube labeled “E” and inserted it into the spectrometer. A partner then recorded the absorbance reading for every 20 seconds for a total of 120 seconds. After the experiment, a brown color in the tube should be observed to indicate the reaction was carried out. Using sterile techniques, any excess liquid left was disposed
The experimental procedures for Lab 2 were provided on Blackboard labelled as “Pre-Lab 2: Techniques & Measurement”.
The group started by testing the controls. The group then added two drops of a blood sample to spot A and spot B on a blood typing slide. After the blood samples were added to the spots, the group added the appropriate antiserum to each of the samples. The group stirred each blood type and antiserum for 10 seconds with a clean toothpick.
Next, in the 10% concentrated test tube my group recorded 33 chlorella in the 22x22mm sample and approximately 2,632,471 Chlorella in the test test tube itself. Furthermore, the class average was 42.5 Chlorella in the sample and around 3,390,3947.5 in the test tube. Lastly, with the lowest amount of surviving Chlorella for my group was the 20% concentration of miracle gro. My group’s data had no surviving Chlorella in this sample while the class average had 35.75 in the sample and 2,853,385.25 in the test tube.
Please complete the entire experiment as instructed in the lab manual except for any modifications noted below. Fill out the
The purpose of this lab was to identify the species of an organism from a given TSB broth culture. Each student was given an unknown sample, and each sample had an assigned ‘sample number’ for post-lab verification by the professor. My sample number was #18. A list of possible organisms was given before any tests were run and a chart with the accepted test results for each organism was given as well. To correctly identify the organism, multiple biochemical tests were performed until enough test results were collected to single out the correct organism from the given list. Also given at the beginning of the lab was each samples results for the VP test (negative for
Each potato section was cut to 3cm long by 1cm wide, do your best to make sure that each potato is cut to equal size. Then place a cut potato in each test-tube and immediately mark the height of liquid in each test-tube with the marker and start the stopwatch. After 2 minutes have passed measure the height of the bubbles that form over the line we had previously marked. Record all the results into a table that was provided for. Finally graph the data that was recorded from the test-tubes, use the height of the bubbles as the enzyme
A wide variety of processes had been performed to determine what culture had been acquired in class. My group had acquired culture tube unknown #3. We first isolated the bacteria. In this step we took the broth of the unknown #3 and grew it on an agar plate. The first process that had been performed was discontinuous streaking, and continuous streaking to grow the culture for future use in the lab and to have extra to perform a whole variety of test to determine the unknown. The next experiment that had been performed
The control of the experiment is water. The positive results are the juices that turned into jelly and the negative results were pineapple and kiwi.
In this novel “A Tale of Two Cities”comes two characters C.J Stryver and Sydney Carton who had been working together as Lawyers. Before they were lawyers,Stryver and Sydney were so much of friends and were alcohol drinkers back in Paris. The fact that both Stryver and Sydney work together as lawyers,Sydney became a great successful person than Stryver. All to this,I surely disagree that C.J wouldn’t be much of a great successful lawyer without any of Sydney’s help.
The first experiment begun by filling a 600-ml beaker, almost to the top, with water. Next, a 10-ml graduated cylinder was filled to the top with water. Once water was added to the beaker and graduated cylinder, a thumb was placed over the top of the graduated cylinder. This would ensure that no water was let out and no bubbles were let into the graduated cylinder. Next, it was turned upside down and fully submerged into the beaker. Then, a U-shaped glass tube was attained. The short end of the glass tube was placed into the beaker with the tip inside of the graduated cylinder. Next, a 50-ml Erlenmeyer flask was received. After, 10-ml of substrate concentration and 10-ml of catalase/buffer solution were placed into the flask. A rubber stopper was then placed on the opening of the flask. After adding these, the flask was held at the neck and spun softly
The experiment that the class worked on was about peroxidase. Peroxidase is part of the enzyme group that presents most living organisms (Ahmed, 2013). Peroxidase interferes with the removal of hydrogen peroxide (Ahmed, 2013). Hydrogen peroxide is a toxic product that have normal metabolism before it causes any cell damages (Ahmed, 2013). Peroxidase has two substrate and both of them must present a reaction (Ahmed, 2013). One of the two substrate is H2O2 and other one just depends on the organism or the cell type (Ahmed, 2013). The substrate that the class uses is turnip extract. In the class there were five experiments to do but the class were assigned into groups and each group were going to do two experiment. The names of the experiments are: Baseline, Temperature, and pH.