Photosynthesis Lab Report

Measuring the Rate Oxygen Production using an Oxygen Electrode Chamber in Photosynthesis of Spinacia oleracea with Varying Light Intensities

To measure the rates of photosynthesis by measuring the change in CO2 levels in the air that is in the LeafLab chamber.

This is because the plant was in the test tube by itself, and placed in the light so the elodea could freely photosynthesize, which would eliminate a large portion of the carbon dioxide within the tube.

Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen and nitrogen and are bound more tightly to the paper than the other pigments.

Then he micro-centrifuge tube was placed into the water bath that was filled with 20°C water. 2 cm of the tube was sticking out of the top of the water bath. Then the oxygen sensor was placed, and tightly sealed into the test tubes opening. Then the walls of the water bath were completely covered with aluminum foil. This was to insure no light could reach the leaf.

The data in the graph and table does not support the hypothesis that if spinach leaves are given red and blue light, then the blue light leaves’ photosynthetic rate will be higher than the red plant’s because blue has a shorter wavelength of light and a higher absorbance rate and therefore has a higher level of energy in order to help the plant photosynthesize most effectively (Braddock). As shown by table 1’s photosynthetic rates, the blue rate of photosynthesis was lower than the red with -0.00559 being 0.00106 points lower than that of the red photosynthetic rates. This shows that from minutes 0-22, the red light’s photosynthetic rate was (while still lower than the rate of cellular respiration) higher than the blue light’s rate of

Follow the steps detailed in the first experiment to test the effects of an increase in light intensity on photosynthetic rates in corn (a C4 plant).

photosynthesis happens in two stages: light reaction and carbon fixation also known as calvin cycle.light reaction TAKES PLACE IN THYLAKOID USE light energy to produces atp and nadph whereas, calvin cycle takes place instroma uses energy derived from light dependent reaction to make GA3P from CO2 ( Bio166 lab execise manual, 2015). the purpose of this experiment was to separate plant

Based on your data, draw a conclusion regarding how light intensity affects the rate of photosynthesis.

To performed the experiment, a volumeter was set up to measure the net oxygen production under white light, then a second step was followed to measure oxygen consumption under dark conditions (oxygen production only happens in the presence of light and oxygen consumption in the presence of dark light) and finally, a third step consisted of recording the measure of the net oxygen production under the presence of green light.

The purpose of this experiment was to determine whether spinach leaves would produce more CO2 if spinach was under blacklight, versus in the the dark. It was hypothesized that if spinach was put under blacklight, then spinach would produce less CO2 because there would be some light energy entering the plant, and that would cause the spinach to photosynthesis. This would decrease the rate of CO2 production. The data did not support this hypothesis. This investigation was valuable because it showed that respiration was still happening in the dark, which can lead to other investigation. This is also important because this showed the effects of different wavelengths of light on the rate of CO2 production in plants, and info into what might be happening

Cut out parts of leaves to be the testes for the experiment to see if the lab was true. Then we sucked all the oxygen out of the leaves and placed them under light and under darkness. This was to test to see if light and water were needed.

Purpose: In doing this lab, the objective was to find how the intensity of light effects the of the products of photosynthesis. This lab will measure to what extent production of photosynthesis increases when light intensity is increased. This experiment will not only provide a visual representation of the process, but will also allow for further examination of the relationship between photosynthesis and cellular respiration. Introduction: The process of photosynthesis uses energy from the sun to produce glucose for plants, and cellular respiration takes glucose and oxygen to form carbon dioxide and water.

Purpose: The purpose of this lab was to determine how light affected the amount of oxygen gas produced over time. The amount of oxygen gas consumed during cellular respiration and produced during photosynthesis was measured. The experiment also determined the rate of respiration and photosynthesis of a plant.

Recording data: Measure the distance between the lamp and the beaker. It should be 70cm at all times because it is a control variable. This cannot be changed because light intensity affects the rate of photosynthesis. If the plant is too close to the lamp, the results would be most affect by light intensity instead of carbon dioxide and the results would not be accurate.