Analysis of Data:
During Part I, we tested the permeability of dialysis tubing using a combination of calcium chloride and starch dispersion. After waiting one hour we tested the water in the beaker (outside of the tubing) for the presence of calcium ions, chloride ions, and starch. Through this experimentation, we found that chloride ions slightly permeated through the tubing, while starch and calcium ions did not. These findings suggest the calcium chloride broke down into separate ions and allowed the chloride ions to travel across the membrane. In Part II, we observed the process of osmosis using IV solution. After letting the tubing sit in distilled water for one hour, the mass of the bag increased 3%, which suggests that water entered the tubing – making the IV solution a
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A similar process was done in Part III to observe the osmosis of basic vegetables: carrots, cucumbers, and prunes in both water and a sugar solution. The mass of the prunes increased in both scenarios, while the mass of the carrots decreased. As for the cucumbers, the mass increased in the water solution, but decreased in the sugar solution. Lastly, in Part IV, we observed the permeability of gummy bears, ranking the size from smallest to largest. The only solution that caused the bear to shrink, also known as a hypertonic solution, was the 20% salt solution. All the other solutions, apart from corn syrup, produced an instant recognizable size change. After further, more precise examination, the bear in the corn syrup looked slightly bigger than the control. These comparisons and in-depth looks at the soaked gummy bears allowed me to create a
On the second day, the gummy bears were drained from the containers and measured. The first gummy bear that was saturated in distilled water measured 4 centimeters in length and 1.5 centimeters in width. The second gummy bear was 4 centimeters in length and only 1 centimeter in width. Both gummy bears were larger than they were on the first day.
Investigating the effect of different concentrations of glucose on the percent change in mass of Gummy bears according to their respective colours
First, each table received 2 gummy bears. Each gummy bear was measured for their height, width, and thickness in mm. Secondly, 150 ml of water was poured into a cup with your group number on it. Then after we recorded our measurements of the gummy bears, one gummy bear (A) was placed in the 150ml cup of water, while the other gummy bear (B) was placed in the Ziploc bag. The gummy bears were measured again after 24 hours. When we checked the gummy bears after 24 hours, gummy bear B in the bag stayed the same, while the height and width of the gummy bear A in the water grew and the thickness shrunk. Originally, the gummy bear A in the water weighed 2.57 grams, measured 12mm thick, 10mm wide and 17 mm tall. After the experiment, gummy bear A
In this lab we found out that the dialysis bag contained more fluids inside it. This can happen because sucrose is small enough to pass through the selectively permeable membrane. Some errors that might have been encountered during the lab could have been the fact that some of the dialysis bags were not tightly tied with the dental floss, or that the wrong substance was used. Another possible source of error was if the carrot strips had been dehydrated from the beginning of the experiment or were already
In which two of the dialysis bag contain tap water. The three other Bags were 20% sucrose 40% sucrose and 60% sucrose. To perform this lab we prepare each of 5 bags with their designated sucrose solution. In this experiment the dialysis tubing acted as selectively permeable membrane. The dialysis bag allows the passage of water molecule only because the sucrose molecule are too large to pass through the membranes. After filling those bags ,we then placed the bags into five separate beakers. Four of this beakers contained only tap waters, and the fifth beakers contains solution of 60% sucrose. We filled the first dialysis bag with 10 ml of tap water, next we filled the second bag with 10ml of 20% sucrose, the third bag was filled with 10 ml of 40% sucrose solution while the fourth bag was filled with 10ml of Tap water (H2O). We made sure that when filling this bags that we removed as much air as possible before clamping of the end of each bag. We also made sure that all of the bags rest soft and floppy instead of firm to ensure that experiment will work properly. Next we placed dialysis bag ,one through four in their own separate beakers which was filled with tap water (H2O), and our fifth bag was full of tap water was placed in beaker filled with just enough of the 60% sucrose solution to cover the bag. All of these bags remained in their designated solutions for 45 minutes. However, all of this bags were quickly removed every
The molarity of the solutions in each dialysis bag, and the percent change in mass are both included to best show effect of increasing molarities of sucrose and osmosis. The percent change in mass exhibits the process of osmosis, because osmosis must take place for water molecules to transfer from one side of the semi-permeable (in the surrounding distilled cup) to another (within the dialysis tubing), in order to reach equilibrium.
The dialysis tubing will be clamped at one end in order to fill it and then clamped at the other end to seal the filled bag. If the bag is not soft and floppy, the experiment will not work. Blot a bag with a paper towel to absorb the moisture and weigh it, if this blotting process is not done it could interfere with the weight readings creating inaccurate information. After the bags of the solutions are prepared, they will be placed into five different beakers with different solutions. Beakers 1-4 will be filled with tap water and the fifth beaker is filled with 40% sucrose and water. Fill each beaker with just enough water or solution so that the bag is covered and place the bags in the beakers simultaneously and record each time. Every 10 min the bags are to be taken out, blotted, and weighed again before returning them back into their respective beaker for another 10 min. The process is repeated until you have reached 90 min. The weights should be recorded in grams (g).
The main purpose of the experiment was to test the idea that water would move from the higher concentration to the lower concentration. In order to test this theory, we placed potato slices in 7 different containers, each containing different concentrations of NaCl, to measure the weight change from osmosis. The containers ranged from 0M NaCl all the way to .6M NaCl. We measured the potato slices before and after placing the slices in the solutions and recorded the net change in weight to determine the tonicity of the potato cells. Our results showed that the potato slices put in a NaCl solution of .2M or higher lost weight and the potato slices put in a NaCl solution of .1M or lower gained weight. This shows that the osmolarity of the potato falls within the range of .1M to .2M, and it also proves the process of Osmosis by having the higher concentration move to the lower concentration. In addition to this, it can be concluded that the osmolarity of cells can be determined by observing the affects of osmosis.
Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is
I know many students, as well as my self, who aspire to be as hard working as Pavanaj. For many students its a struggle to balance school with work, and still have enough time for sleep, let alone a personal life. Yet, Pavanaj makes it look effortless. He always has his work done and he is always there to help someone in need. Throughout all the chaos of school, from deadlines, to basic organization, to final exams; Pavanaj will have it under control. Not only does it appear that he is always in the eye of the Hurricane, but that he is the one who is calming the storm. It is always a sigh of relief to know that Pavanaj is there to help you solve a problem or work through an issue.
The major objective of the experiment was to test the effect of the concentration gradient on the diffusion rate. It was hypothesized that the greater the stronger the concentration gradient, the faster the rate of diffusion would be. To test this, dialysis tubes were submerged in different concentration fructose solutions. We weighed the tubes at specific time intervals to measure the rate of diffusion of water in each different solution. The results illustrated that increased concentration gradient increases the rate of diffusion of water in the tubes. We concluded that as concentration of the
The death penalty is arguably one of the most controversial issues of our time. Currently, only eighteen states and Washington D.C. have abolished the death penalty. However, the question remains: what does research say for or against the death penalty? In 2009, a study was done that found the statistic that 88% of criminologists believed that capital punishment is not a deterrent to murder (Radelet and Lacock, 2009). The majority of research also points to the conclusion that the journey from arrest, to trial, to execution is extremely expensive, even more so than that of a life sentence. Finally, who considers the people tasked with performing the execution? What about their mental health? Through examining the death penalty’s facts, deterrence,
In this lab we are going to discovery how osmosis works using a semi-impermeable membrane a potato slice. Osmosis is known as the movement of water in and out of a cell. To understand how this works we must understand two terms. Hypotonic means the environment has less solutes compared to the inside of the cell. Hypertonic means that the environment has more solutes compared to the inside of the cell. With osmosis water will always move from hypotonic too hypertonic. So the question is will water move into the potato or out of the potato? Will these results change when placed in different morality of salt water? To calculate these results, we will measure the mass of potatoes cut into equal sizes then soak these potato slices in different morality of NaCl for thirty minutes and measure the mass change in each potato slice.
If the gummy bear is added to the solution, then it will lose its coloring and expand.
The beaker was then filled partially with distilled water; 1 ml of potassium iodide was then added, and the solution was tested for the presence of glucose. This data was recorded in table 1 on the data sheet along with the starting color of both the potassium iodide solution and the glucose/starch solution. The dialysis tubing was then submersed into the beaker containing the potassium iodide solution, and set aside for 30 minutes to allow maximum diffusion.