Ondanseton Gel Lab Report

You can view macromolecules by using a technique called gel electrophoresis. Gel electrophoresis is a process used by scientists to study and view macromolecules. This process separates the different macromolecules and coats the different macromolecules so you can easily see them. A macromolecule is a very large molecule that contains many atoms. Agarose is used in gel electrophoresis to coat the macromolecules.

To prepare the 0.8% w/v gel, a solution of 50 mL of 1x TBE Buffer was added to a flask containing 0.4g of agarose. The solution was carefully swirled around to mix the contents and then covered with clear plastic wrap to be heated in the microwave for 1 minute. Once the solution

-The gel was visualized using ultra-violet transilluminator. 3.20.3

Acquisition of Size of DNA Restriction Fragments With Gel Electrophoresis Min, S., Chen, F., Forlenza, A., Li, M. Frockowiak, L., Lab Section C, 12-13-17 I. Gel Sketch: II. Graph See attached III. Data Table Table 1: Migration Distance and Base Pair Length of Several DNA Fragments Well Migration Distance (cm) Base Pair Length

Agarose Gel Electrophoresis A 0.8% agarose (OminPure/Midsci) with 1X TAE buffer, and 200ng/mL EtBr (OmniPure) was prepared. After loading 10µL of samples and 10µL of 1kb ladder (NEB) and using 1X TAE buffer, gels were run at 100V for 60 minutes (Fisher Scientific). The gel electrophoresis of new wild-type Habibi DNA also had 6X loading dye added to the sample. A UV transilluminator (Red) was used to view the gels.

The concentration of drug present was maintained at the same level throughout all experiments via implanting an equal mass of 10mg into each liposome (Table 8), in order to control the experiments as a variation in this value could lead to higher or lower susceptibility to more efficient entrapment. A higher volume of drug in the liposome was expected to lead to a lower entrapment efficiency due to the increased diffusion gradient and a higher pressure in the aqueous core. The temperature used for rehydration (60C) was chosen based on being higher than the phase transition temperature of the lipid. The reason for this temperature was that, under these conditions, the gel is converted to a liquid.

Size-exclusion chromatography (gel filtration) This technique is used to separate larger proteins from small ones by using a minimal volume of eluate, since the larger molecules travel faster through the cross-linked polymer in the chromatography column. The large proteins do not fit into the pores of the polymer whereas smaller proteins do, and take longer to travel through the chromatography column, via their less direct route. Eluate is collected in a series of tubes separating proteins based on elution time.

, 50%, 75%, 100%, 125% for both the drug and the total drug content were determined as discribed for formulation. The percentage recovery was determined and the results are givan in Table

Since the column was already set up before hand, the first step in this experiment was to prepare the standards. We obtained a standards solution of saline containing both Blue

Standard solutions containing 1000, 500 and 500 μg.mL–1 of KTC, PHE and CPM, respectively were prepared separately by dissolving the reference materials in methanol. Regarding the stability of solutions of the drugs, stock solutions were stored at 4°C in amber glass vessels and were found to be stable for at least 10 days. The working solutions were prepared by dilution of the standard solution with methanol. Different volumes corresponding to concentrations in the range of 12 - 50, 7.5 - 27 and 9 - 27 μg.mL–1 for KTC, PHE and CPM, respectively were diluted with methanol in 10-mL volumetric flasks.

There are many different types of pills that humans use for various reasons, and each one is designed to cure or contain a certain problem or pain. This form of medication is usually compacted in a small tablet, sometimes with a coating on top, and meant for the user to swallow as a whole without allowing it to dissolve before entering the stomach. However, considering that people seem to want the fastest relief possible that they can get from their bodily ailments, the research question of this experiment was: Which class of pills dissolve the fastest?

3 Average Average melting point of diltiazem hydrochloride was found to be ----- Solubility analysis of Diltiazem hydrochloride Sl. No Water Methanol Phosphate buffer pH 7.4 1 2 3 Average Calibration curve of diltiazem hydrochloride in water Sl No Conc in µg/ml Absorbance 1 4 0.1699 2 5 0.2262 3 6 0.2746 4 7 0.3226 5 8 0.3756 6 9 0.434 7 10 0.5123 Standard Graph of diltiazem hydrochloride in phosphate buffer pH 7.4

The weight of the vials and the weight of vials plus SRP were measured as initial value for one day. The water was let evaporated for 24 hour and the next days the weight was measured until there is no water loss. This process was repeated for different concentration of Sylgard-309 non-ionic surfactant from 10% (w/v) to 50%

Table: 40 Evaluation of Bilayer Tablets of Metoprolol Succinate SR and Hydrochlorothiazide IR after Stability Study at 40°C±2°C/75% ±5%RH

Since ancient times different pharmaceutical formulations were used to deliver different drugs for treatment of various diseases, which played a role in saving the life of thousands of people. For example since discovery of oral rehydration sachets many lives have been saved from dehydration caused from severe diarrhea. Oral liquid preparations have been a preferable choice for many patients and provide better compliance than other dosage forms due to the easiness of administration.