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Nt1310 Unit 1 Lab Report

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I received 2.5 out of 3 possible points for this question. Based on the TA’s notes, it seems as though I lost ½ of a point for not saying that “more ddNTPs will result in shorter sequences”. However, in my answer, I did in fact say that increasing the ratio of ddNTPs to dNTPS will cause DNA sequencing to stop quickly, namely at the first few nucleotides if many ddNTPs are added. Because I included such information in my answer, and given the accuracy of the rest of my answer, I’m not sure why I lost ½ of a point and instead believe that I did not deserve to lose any points, or at least did not deserve to lose ½ of a point. In other words, although I believe this did not warrant any loss of points, I certainly believe it didn’t warrant a loss …show more content…

I received 2.5 out of 3 possible points on this question. First, as I approached this question, I remembered our discussion in class of methods for determining which stage of the cell cycle cells are in, and how doing so indicates growth. In class, you mentioned that cell size can be used to determine which stage of the cell cycle certain cells are in. Although you said that measuring the amount of DNA is a more reliable method, you nonetheless said that cell size can be used for this. Some of the answers on the answer key are methods used to determine which stage cells are in, as doing so can indicate whether or not cells are growing. This was the case for 2 of my correct answers (for example, I mentioned using formation of the mitotic spindle as a way to measure cell growth, as doing so measures a cell's’ entrance into M-phase, which inherently indicates cell growth). So, I am not sure why measuring cell size is not considered as a sufficient answer for part of this question. Q8. I received 2.5 out of 3 possible points for this question. I have a difficult time understanding which parts of the answer provided on the answer key are important, and therefore question if not mentioning decreased telomerase activity was or was not worth a deduction of 0.5

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