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Lab Report Chromatography

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Procedure: First, we began the lab with the chromatography strip positioned 152 mm tall and 19 mm wide. Using a ruler and pencil, we drew 15 mm from the bottom of the paper across the width. We measured 9.5 mm from the edge and placed a dot with the pencil on the line to mark the starting point. The, we measured 20 mm from the top of the strip and folded across the width of the strip. These few steps were repeated with a second strip. Next, the red and purple dyes were obtained. Using 2 clean toothpicks, the chromatography strips were spotted by placing the toothpick into a dye and then touch the tip gently onto the designated pencil dot. The sample was allowed to dry. The spotting steps were repeated 2 to 3 more times to increase the concentration of …show more content…

While the samples are drying, two 250 mL Erlenmeyer flasks and watch glasses were obtained. Then, we poured 20 mL of 2% chromatography solvent (isopropyl) into each flask. We covered each flask with a watch glass. Once the chromatography paper is dry, the watch glasses were removed. Next, the strips were carefully hanged into the 2 flasks with the sample side down. We made sure to keep the sample spots above the level of the solvent. Afterwards, we placed the watch glasses back on top. The chromatography was allowed to develop. We recorded observations of dye samples as the solvent traveled up. When the solvent was within 1 to 2 cm of fold in strip, the run was stopped by removing strips from flasks. With a pencil, we lightly drew a line to mark the distance the solvent has traveled, which is called the solvent front. Using a ruler, we measured the distance from the pencil line at the bottom of the strips to the solvent front and recorded our observations in mm. Lastly, the shape of each dye bands was traced with a pencil to mark its locations on the strips. The distances in mm that each dye band traveled were measured and recorded. we made sure to measure from the bottom of the strip to the center

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