M & M Food Dye Chromatography

Chemistry 262 Lab TA: Mona Khorani Yuen Wai Ng Group Members: Mihir Palan and Haelyn Epp Group 6 February 20, 2018 Establishment of Food Dye Content and Concentration in Purple Gatorade Abstract The experiment of determining the different food dyes present in the purple Gatorade and then creating a solution with the correct color and concentration of the Gatorade chosen. Trials were done with blue and red dyes to dilute the concentrations of the respective dyes in order to find the amount of dyes needed in the resulting solution based on the absorbance and concentration.

Answer: Once the chromatogram has been completed and is ready to be measured and calculated, on the plate that was used to perform the chromatogram you should see where the red and blue have completely separated. The red food coloring dye should be lower on the plate than the blue food coloring dye.

Thin Layer Chromatography (TLC) works in relation to the polarity of chemicals. A plate is first covered with aluminum foil or silica etc. and has solutions of varying polarities placed upon the aluminum foil or silica. When placed in a in a puddle of solvent that moves up the plate, the different inks i.e. the solution will move up the place based on their Rf values. Adherence increases with increasing polarity, so the less polar compounds will be carried farther by the solvent. Eventually the dyes will separate into their compontnets, which can be visibly seen. This is then used to determine who the ink of the unsigned note belonged to along with the pen that it belonged

In thin layer chromatography a stationary phase, silica gel with a glass backing, is dotted on a pencil drawn

Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct?

The similarities between column and paper chromatography is that both have a stationary and mobile phase. The stationary phase in the paper chromatography is the paper, and in the column is the sodium bicarbonate. The mobile phase in the paper chromatography is the movement of the solvent, .3% NaCl, and the column is the different solutions added. Column chromatography is basically the same as paper chromatography, except that the dye solution would have to be added individually rather than being able to do it all at once, like it does when using chromatography

Surfactant is an essential component for the respiratory system to function properly. Knowing the physiology of surfactant in the alveoli is important to know when learning the structure of the air sacs and how they work. This experiment is designed to make the understanding of surfactant in the alveolar film easier to learn, because it's not very simple. Surfactant is a detergent-like substance produced by the Type II alveolar cells in the walls of the alveoli. Surfactant is produced to reduce the surface tension of the water molecules that primarily compose the walls of the alveoli. For this experiment, we will be using milk and food coloring to represent the water (milk) and gas (food coloring) in

After the invention of lithography between 1796 and 1798, came the improved and colorful chromolithography in 1837. This method of lithography was an improved way of making posters with a lot more color. It replaced painting posters by hand, which used to be taking black and white lithographs and coloring them by hand. Lithographers wanted to find a way to print on flat surfaces with the use of chemicals instead of relief printing. The process can be very time-consuming and cumbersome, contingent upon the skill of the lithographer. Depending on the number of colors present, a chromolithograph would take months to produce.

When my now six-year-old son was three, he was very hyperactive. He could not sit still and was always very emotional. Often, it would take a lot of effort to get him to do anything he was told. There were several times where he needed to be physically restrained in order to get him to calm down. After a few episodes at his preschool, one of the administrators suggested that we monitor his diet and remove synthetic food dyes from it. After 3 days of him not having any food with any synthetic dyes in it, he was a different child. He was calm, easy to handle, and his teachers said he was a real pleasure to have in school. After several months of him not having any synthetic food dyes,

It is possible to separate these pigments from each other using a technique called paper chromatography. In this process, plant tissue extract is applied to a piece of chromatography paper. “A solvent is allowed to travel up the paper, and if the pigment is soluble in the solvent, it will be carried along with it.” (Benya, 2009) Different pigments have different affinities for the solvents or polarity and will travel at different rates. Chlorophyll, anthyocyanins, and carotenoids are typically non-polar.

The next step was to place the strip of chromatography paper on a paper towel. Then dip a capillary tube into the plant pigment extract (spinach pigment extract) provided by the teacher. The tube will fill on its own. We applied the extract to the pencil line on the paper, blew the strip dry, and repeated it three to four times until the line on the paper is a dark

Gel-Filtration Chromatography is a commonly used method used in order purify a protein from a mixture, by means of separations. Different biomolecules differ in size, or their molecular weight. In the gel matrix inside the chromatography column, there are gel beads which are porous to allow certain sized molecules to enter. The molecules that are able to enter the pores of the gel, are held in stationary phase and will elute from the column later on, these are usually smaller, to medium sized molecules. Larger molecules that are not able to fit in the pores will elute out of the column first, they are involved in mobile phase where they just go straight through the column without interacting with the gel beads. Smaller molecules will have a higher elution volume, while the larger molecules will have a lower elution volume. The volume to elute the protein is inversely proportional to the molecules size.

The reason for conducting the research is to know how the colors of our favorite candy-coated sugary rewards came to be and that method of knowing is what we call “Candy Crhomatography”. Chromatography by definition according to Webster’s dictionary means “ a method for separating the constituents of a solution (gas or liquid) by exploiting the different bonding properties of different molecules”. In

    The process of bioprinting is actually quite simple. The bioprinter has three heads; the first two output the specific cells needed and the third one dispenses “bio paper” to support and protect the cells during printing. Bio paper is a dissolvable

Chromatography is a separation technique in which the mixture to be separated is dissolved in a solvent and the resulting solution, often called the mobile phase, is then passed through or over another material, the stationary phase. The separation of the original mixture depends on how strongly each component is attracted to the stationary phase. Substances that are attracted strongly to the stationary phase will be retarded and not move alone with the mobile phase. Weakly attracted substances will move more rapidly with the mobile phase.