preview

Inflammatory Response Essay

Decent Essays

While mounting of inflammatory response is critical in eradicating bacterial infection, systemic bacterial infection can lead to an acute pro-inflammatory response, which may result in sepsis. Currently, no effective therapy is available to inhibit the activation phase of the acute inflammatory response to infection. A new group of host-protective lipids termed 13-series resolvins were demonstrated to promote bacterial phagocytosis, reduce recruitment of neutrophils to the site of inflammation, reduce inflammasome activation and augment host recovery from systemic infection by accelerating the resolution of the acute inflammatory response (48). I.p. injection of resolvins into mice infected with E. coli showed 40% increase in survival …show more content…

MULTI-OMIC APPROACHES TO IDENTIFY HOST TARGETS THAT MEDIATE BACTERIAL INFECTION Advanced high-throughput technological developments in the fields of transcriptomics, proteomics, metabolomics and imageomics (high throughput, high-content imaging (HCI)) provide an unsurpassed opportunity for identifying host-pathogen interactors and characterizing gene functions in the context of bacterial infection. Transcriptomics studies enable quantitative measurements of the dynamic expression of the mRNA molecules and their variation in different states at the genome scale (49, 90). Proteomics studies [e.g. mass-spectrometry (MS) and Reverse-phase protein microarray (RPMA)] facilitate the characterization and quantitation of proteome changes from complex samples (17, 38). Metabolomics studies reveal metabolites (including lipids, small molecules) that are generated in response to infection (29). Importantly, technological advances related to assay miniaturization, high-throughput and automated image acquisition and quantitative analysis have made it possible to extract hundreds of functional and morphological features that are associated with bacterial infections (9) (Figure 3). Specific HCI imaging parameters has been applied in host-directed therapeutic discovery to study effects of perturbations in bacterial infection cycle (13, 16, 41, 60, 79). Analysis of the data derived from the omic studies will require

Get Access