Samples: - Six different batches of recombinant HVT-H5 avian influenza vaccines obtained from office of receiving samples in central laboratory for evaluation of veterinary biologics (CLEVB), Abbasia. - A/chicken/Egypt/12378 N3-CLEVB/2006/H5N1 strain used as positive control in real-time PCR (Standard sample). - Negative H5 samples. 3.1 Detection of H5 gene insert (identity) by 3.1.1 q real time PCR: 3.1.1.1 Extraction of nucleic acid 3.1.1.1.1 Materials: - DN easy Blood & tissue Qiagen extraction kit. - QIA amp Viral RNA Mini Kit (quiagen kit) - Absolute ethanol. - QIA amp Mini spin column. 3.1.1.1.2 Equipments: - Micro centrifuge tubes 1.5 ml. - Vortex. - High speed centrifuge. - Uni-channel 100-1000 μl. - Uni-channel 5-50 μl. - Water bath.
The tensile testing was done on the three composite specimens (90°, and two 45°) were completed with a servo-hydraulic load frame with a wedge. The one in the lab was the MTS 647 hydraulic wedge grip and an 810 material test system. The specimens had strain gages with a Wheatstone bridge to collect data such as time, distance, load, axial strain, and transverse strain. From the strain gages, evidence can support how and when the specimen material failed under the stress being applied to it. The test was run for three times on three different specimens. The first specimen that was tested in the hydraulic load was the 0°/90° specimen, which is made of carbon and epoxy laminate composite.
Remove the cockroach from it habitat and submerge it in ice water for a few seconds to paralyze it. After the cockroach is paralyzed, using the surgical tools cut a leg (at the hip). The leg should be placed on the cork of the spike box, allowing a bit of the leg to overhang. The stimulation electrode should be placed on the femur and COXA then the electrodes should be connected to the output of the TENS electrical stimulator. Using the provided LabVIEW program, the onset stimulation delivered to the leg as well as its duration can be controlled. The stimulator should be turned on with the correct corresponding channels by turning the knob clockwise, the current intensity of the impulse increase further when the knob is turned clockwise. A
The outer membrane in the cyanobacterium cell (Phormidium uncinatum) is the structure that makes the cell gram negative. On the other hand, the characteristic that makes the cell gram positive is the thick peptidoglycan layer.
In this lab we will be studying electric field plotting through the use of various apparatus. The equipment we will be using includes a digital voltmeter, voltage probes, carbonized acetate sheets, battery, and other tools of measurement. The goal of the lab is for us is to be able to plot lines for charge distributions and to create electric field lines by providing an electric current through carbonized sheets. Once we have completed constructing the electric field lines for the plate charge distribution, we will continue to calculate for the electric field (E = Vx) and begin to plot for the dipole charge distribution. When we have collected all the data needed, we then will finish calculating for all the remaining variables and complete the rest of the lab report.
FIGURE 1.7- A magnified view of a single lenslet shows a portion of an aberrated wavefront (red curve) passing through. If the wavefront had been flat (aberration-free), it would have focused to a point on the video sensor (CCD) on the lenslet's optic axis (yellow dot). Refractive errors distort the wavefront out of a plane, so a wavefront portion (red curve) enters the lenslet curved and tilted. The light will therefore be focused to another part (red dot). The dot will be shifted by an amount that is in direct proportion to the wavefront tilt.
In the LULC/Soil/Slope tool box, the last option is slope tab. User has to select to number of slope classes for the watershed. Two options are available to define the slope discretization in the slope definition tool box. First one is single slope and second one is multiple slopes. If user select single slope in the tool box, it creates slope range class 0-999%, if user selects multiple slopes, the tool allows to create 5 classes. For the kaddam watershed five slope classes has been selected in the tool box. The classes are 1) 0-5% 2) 5-10% 3) 10-15% 4) 15-35% and 5) 35-9999%. The slope map of the kaddam shown in the
First, we need to plug in the variables into the numbers from the information to create our first equation for the system. 3j (glasses of orange juice) + 5p (5 pancakes) = 7.60. Now, plug in the variables into the numbers from the information for the second equation for the system. j ( a glass of orange juice) + 2p ( 2 pancakes) = 2.90.
The goal of this lab was to expand and centralize our infrastructure by adding a Windows Server 2012 VM and configuring it with a number of key services. Some of the services that we deployed on the Windows 2012 VM had previously been configured on other machines, namely the RHEL VM from Lab 2. We began by installing and configuring the server itself, ensuring that we provided it with ample memory and processing power. Next, we configured DHCP, disabling the DHCP service running on our RHEL VM. We then continued by installing Active Directory and DNS on the Windows 2012 VM, disabling the DNS server running on the RHEL VM in the process. The DNS server on RHEL would later be reactivated as a secondary (slave) server. We then proceeded to set up monitoring for both DHCP and DNS. This monitoring was configured locally on the Windows 2012 VM using the Performance Monitoring utility, and also remotely via OpsView. Finally, we installed Microsoft Baseline Security Analyzer on the Windows VM. This was the first lab where it felt like we
The estimated sample to be taken should be 9 lettuce plants/ roots to have the 95% level of confidence and within the 10% of population mean.
From the Client VM, clear Internet Explorer’s cache (or completely close and reopen IE) and go to http://10.0.0.1 What do you notice? Record your observations. These observations will help you with your lab report. The web page does not load stating “Internet cannot display the web page.”
The bitlockertogo.exe is an executable file in PCs with Microsoft Windows XP, 7, MAC operating systems installed in them, errors are associated with Microsoft bitlockertogo exe mui, bitlockertogo.exe autorun.inf and to fix them the PC users can free download, install and use BitLockerToGo.exe error repair tool like VSKsoft.
Figure~\vref{fig:res}, shows the characteristic functions of the classes of the linear power amplifiers in which the active device operates as a current source. Its major disadvantage is holding a voltage to the transistor terminals during the conductance of the current, resulting in power dissipation. As shown, classes A and B are linear, while class C is incapable of storing amplitude information, hence often not be considered one of these classes.
I received 2.5 out of 3 possible points for this question. Based on the TA’s notes, it seems as though I lost ½ of a point for not saying that “more ddNTPs will result in shorter sequences”. However, in my answer, I did in fact say that increasing the ratio of ddNTPs to dNTPS will cause DNA sequencing to stop quickly, namely at the first few nucleotides if many ddNTPs are added. Because I included such information in my answer, and given the accuracy of the rest of my answer, I’m not sure why I lost ½ of a point and instead believe that I did not deserve to lose any points, or at least did not deserve to lose ½ of a point. In other words, although I believe this did not warrant any loss of points, I certainly believe it didn’t warrant a loss
The FDA approved in 2003 the commercialization of FluMist® (MedImmune) the first influenza vaccine with attenuated virus. This trivalent vaccine is administrated via intranasal spray because the virus replicates in cells lining the nasopharynx [21–23]. Use of live-attenuated vaccine has been limited to individuals two through forty-nine years of age based on a failed efficacy to prevent febrile illness in adults fifty through sixty five years of age [22]. As in the TIV the first step for the vaccine production is the generation of the viral seeds that presents the circulating HA and NA and six internal segments (PA, PB1, PB2, NS, NP, M) from the cold-adapted (ca) A/Ann Arbor/6/60 or ca B/Ann Arbor/1/66 (the master donor virus for LAIV (MDV)) [24, 25]. The manufacture of approved FluMist® is also based on the injection in embryonated eggs of the reassortant virus and the recovery of the allantoic fluid with the screening of the reassortant that grows well at 25ºC (cold-adapted) and present restricted growth (ta) at 37-39ºC and is attenuated (att). The vaccine is tested in the ferret model [22].
The goal of this lab was to familiarize myself with the RLES vCloud Director, the vApps, and building virtual machines using RLES. I learned how to work with Red Hat Enterprise Linux 7 to build and customize servers built to meet the needs of my virtual environment. I also experimented with configuring pfSense to be my gateway between the virtual network and RITs RLES infrastructure. There was also the introduction of setting up both a wiki and a monitoring solution, which I chose to use MediaWiki and Nagios Monitoring Solution for my services.