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Hvt1310 Unit 4 Lab Report Sample

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Samples: - Six different batches of recombinant HVT-H5 avian influenza vaccines obtained from office of receiving samples in central laboratory for evaluation of veterinary biologics (CLEVB), Abbasia. - A/chicken/Egypt/12378 N3-CLEVB/2006/H5N1 strain used as positive control in real-time PCR (Standard sample). - Negative H5 samples. 3.1 Detection of H5 gene insert (identity) by 3.1.1 q real time PCR: 3.1.1.1 Extraction of nucleic acid 3.1.1.1.1 Materials: - DN easy Blood & tissue Qiagen extraction kit. - QIA amp Viral RNA Mini Kit (quiagen kit) - Absolute ethanol. - QIA amp Mini spin column. 3.1.1.1.2 Equipments: - Micro centrifuge tubes 1.5 ml. - Vortex. - High speed centrifuge. - Uni-channel 100-1000 μl. - Uni-channel 5-50 μl. - Water bath.

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