Flow Diagram Lab Report

2) Record the shape of the bacteria, the arrangement of the bacteria, and the gram staining characteristics.

The identification of unknown organisms carries important ramifications that can be applied to many real world scenarios. In keeping with quality assurance beverages, food, cosmetics, and other products are frequently inspected for contaminants resulting from a presence of pathogenic bacteria. In medicine, a physician’s diagnosis and consequent treatment is largely determined from samples collected from infection sites that have been analyzed using microbial tests.

Many tests were completed on the unknown such as gram staining and inspection under microscopes to find whether the bacterium is gram positive or gram negative. Chemical resistance tests were also performed to see if certain chemicals affected the unknown growth or if it didn’t affect the bacteria at all. Each biochemical test

The next step of the project included preparing a Gram stain to discover the cell shape, arrangement, and if the bacteria is gram positive or

On your own and without assistance, complete this Lab 1 Answer Form and submit it via the Assignments Folder by the date listed on your Course Schedule (under Syllabus).

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.

Our research on recombinant DNA mainly consisted of two experiments: Transformation and gel electrophoresis. In our first experiment, four microfuge tubes were given to us: pKAN DNA, pAMP DNA, unknown DNA, and a TE buffer without DNA. The two positive controls, pKAN and pAMP, consisted of an antibiotic resistance gene respectively to their name. The pKAN plasmid contained the gene resistance for kanamycin while pAMP carried the gene resistance for ampicillin. The negative control, TE, only contained buffer without DNA. The fourth tube was our unknown plasmid, which was either pKAN or pAMP; and by way of artificial transformation, we would be able to initiate the identification of our unknown plasmid.

The objective of this experiment is to identify the organisms of two unknown bacterial cultures. Students must identify the species of the unknown bacteria by utilizing the techniques and information learned in previous laboratory exercises. These techniques include streaking for isolation, Gram staining, and specific biochemical tests. Students are given a map known as a dichotomous key, a guide in determining the identity of their unknown sample.

Previously, detection and recognisation of bacteria were based on conservative tube-based biochemical reactions, and their results were compared to historical charts of expected biochemical reactions. Beacause of the need for quicker, simpler methods,

During the course of this lab, we have learned to utilize the flow process chart to break down the operation into its basic elements. We then made observations of the chart, in order to determine recommendations for increasing the efficiency of the operation. In the course of our operation we were able to determine the average weight of the three cell phones to be 201 g.

coli, and the sample. This dish will allow mutagens in the sample to interact with the E. coli and will allow streptomycin to differentiate the resistant (mutated) and sensitive (normal) E. coli. The top of the test tube containing E. coli was sterilized and 100 µL of E. coli was added using the same methods as for dish 1. The spreader was then sterilized and the E.coli was spread using the same method as in dish 1. The tweezers were sterilized and the sample was added using the same techniques used for dish 2.

One of Falkow's first and key discoveries, around the early 1960's, was finding episomic pieces of DNA that can be replicated independently of the chromosome1 and that different types of bacteria (Salmonella Typhosa, Serratia Marcescens2, Escherichia coli3, Proteus I.4) may transfer and acquire these genes amongst them. This DNA piece, which is the plasmid, and its characteristics and manipulation have redefined the way researchers use molecular biology, and the

: In the field of microbiology, there are times when a sample will contain more than one species of bacteria. The goal is to separate each bacterium and culture them independently from one another to identify them. This was the objective of this lab. Each stock contained two unknown bacterium, and the possible unknowns were Eserichia coli, Enterobacter aerogenes, Enterobacter cloacae, Serratia marcescens, Proteus vulgaris, Klebsiella pneumoniae, Shigella flexneri, Shigella sonnei, and Salmonella enterica. The tests available were MacConkey agar, Endo agar, Hektoen Enteric agar, Tryptone Soya Agar (TSA), carbohydrate sugar broths, Triple Sugar Iron (TSI) agar, decarboxylase broths (arginine, lysine, and ornithine), Simmon’s Citrate Agar, urease

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a

They anticipated that compounds selecting against antibiotic resistance did so based on the bacteria’s physiological interactions with that of tetracycline. The pilot screen results inferred that soil microbes were secreting both forms of the selection-transposing compounds. This indicates that nature has advanced collections of chemicals which offset antibiotic resistance. The results were consistent with the prediction. Using their assay, they discovered that making inverters of selection for tetracycline resistance seemed to be common in soil microbes. Majority of the hits seemed to prefer selection against tetracycline even in the presence of a specified amount of tetracycline. The fusion of compounds that select for or against resistance could provide insight into the concurrence of sensitive antibiotic species found in the wild.