Foreign References:JP0180982 September, 1985 71/16 JP63260885 October, 1988 JP4182377 June, 1992 TW183699 May, 1992 Other References:CA 94(7):46144u, Natural Fertilizer Composition Hamaoka, 1980. Primary Examiner:Lander, Ferris Attorney, Agent or Firm:Young & Thompson Claims:What is claimed is: 1. A method of producing an organic fertilizer by using fish, said method comprising the steps of preparing a raw material containing more than 80% of fish heads, boiling the raw material to obtain a stock, removing solids and oil contents from the stock to obtain stock liquid, thereafter treating the stock liquid in a decomposition tank with decomposing enzyme for enzyme decomposition, filtering the enzyme-decomposed stock …show more content…
Then, solids and oil contents are removed from the boiled stock to obtain a stock solution and this stock solution is supplied to a decomposition tank to treat it by enzyme decomposition. In the aforementioned enzyme-decomposition step, enzyme decomposition may be carried out by adding one or more than two protein decomposing enzyme, fat decomposing enzyme and the like to the stock solution in the decomposition tank. A preferred decomposing enzyme used in the decomposition tank is a bacterial protein decomposing enzyme, such as NEOVITALASE SUPER (which is a trade name manufactured by Towa Koso in Japan). With the use of the aforementioned enzyme, gelatin and protein are rapidly decomposed in the stock solution, and a separation operation in a filter can easily be carried out. Enzyme decomposition in the decomposition tank is carried out by increasing a temperature of the stock solution to about 50°-60° C., and keeping it for about 30 minutes to 3 hours, preferably 1-2 hours, and after decomposition, the temperature is raised to higher than 95° C. to deactivate the decomposing enzyme. The stock solution enzyme-decomposed as described above is separated into a solid content and a liquid content and then the separated liquid content is filtered to form a clarified liquid.
In life, ones fate is already set. The choices one makes influence everything else in their life. Often, fate throws unexpected situations at you and one must learn how to interpret the reasoning behind it. Many believers in fate think for every bad situation that occurs a positive one will follow. Fate is something that you can’t control. All actions whether they may be in the past, present, or future occur because they are meant to. Everything happens for a reason, and everyone handles situations differently.
Decoction, 1 1/2 oz. of the root, boiled in a quart of water down to a pint.
Salting out is the first isolation technique carried out for this lab. This isolation technique separates the Rubisco based on solubility. The method of salting out requires an addition of salt such as ammonium sulfate to the solution containing protein Rubisco to allow precipitation (Duong-Ly & Gabelli, 2014). The additional of ammonium sulfate to the Rubisco
If temperature of the water(enzyme environment) is increased to 35°C, then the enzyme activity will
Jamie Ford’s book "Hotel on the Corner of Bitter and Sweet," is a story about the experiences and hardships that Japanese-Americans suffered during World War II. The protagonist Henry, a twelve-year old Chinese-American boy at the time of the war, reacted with more than just curiosity. The story opens with the news that belongings of several families who were interned is more than just curiosity; he had a deep personal connection to the events of the past and the story uncovers his memories. Henry had done a good job of burying his forgotten dreams, much like the artifacts found in the basement of the Panama Hotel. But just as in real life, when people try to ignore past events, the truth that lies in their heart cannot be denied. Sometimes, it can be something simple, like a parasol, to unlock those truths, but in Hotel, certain objects unlock the symbolism of life itself. Objects are powerful, as they represent not only moments in time, but people 's emotions, motivations, and ultimately, their identity.
Enzymes will denature if they get too hot or cold or if the pH of the solution they are in is too high or too
Students will be observing normal catalase reaction, the effect of temperature on enzyme activity, and the effect of pH on enzyme activity in this experiment. The enzymes will all around perform better when exposed in room temperature than when it is exposed to hot and cold temperatures. This is based on the fact that the higher the temperature, the better the enzymes will perform, but as the temperature reaches a certain high degree, the enzymes will start to denature, or lose their function.
After the substrate solution was added, five drops of the enzyme were quickly placed in tubes 3, 4 and 5. There were no drops of enzyme added in tubes 1 and 2 and in tube 6 ten drops were added. Once the enzyme solution has been added the tubes were then left to incubate for ten minutes and after five drops of DNSA solution were added to tubes 1 to 6. The tubes were then placed in a hot block at 80-90oC for five minutes. They were then taken out after the five minute period and using a 5 ml pipette, 5 ml of distilled water were added to the 6 tubes and mixed by inversion. Once everything was complete the 6 tubes were then taken to the Milton Roy Company Spectronic 21 and the absorbance of each tube was tested.
If necessary the centrifuge can also be used to further separate the two layers. A final means of drying the ester product is the addition of granular sodium sulfate.
The purpose of this lab is to examine the specificity of the lactase enzyme to a specific substrate and how it can denature due to the rise in temperature.
Once cooled, the mixture was then transferred to a separatory funnel using the funnel while avoiding adding the boiling chip. 10 ml of water was then added to the mixture. The mixture was gently shaken and the phases were allowed to separate. The funnel was then unstopped and the lower aqueous phase was drained into a beaker. 5 ml of 5% aqueous NaHCO3 was added and then shaken gently. A great deal of caution was taken into consideration because of the production of carbon dioxide gas which caused pressure to develop inside the funnel. The pressure needed to be released so the funnel was vented frequently. The phases were allowed to separate and the lower aqueous phases was drained into the beaker. After draining, 5 ml of saturated NaCl was added to the funnel and then shaken gently. Once again, the phases were allowed to separate and the lower aqueous phase was drained into a beaker. An ester product was produced and was transferred into a 25 ml Erlenmeyer flask. This organic product was then dried over anhydrous Na2SO4 to trap small amounts of water in its crystal lattices thus removing it from the product. Finally the ester was decanted, so that the drying agent was excluded from the final product.
14 mL of 9 M H2SO4 was added to the separatory funnel and the mixture was shaken. The layers were given a small amount of time to separate. The remaining n-butyl alcohol was extracted by the H2SO4 solution therefore, there was only one organic top layer. The lower aqueous layer was drained and discarded. 14 mL of H2O was added to the separatory funnel. A stopper was placed on the separatory funnel and it was shaken while being vented occasionally. The layers separated and the lower layer which contained the n-butyl bromide was drained into a smaller beaker. The aqueous layer was then discarded after ensuring that the correct layer had been saved by completing the "water drop test" (adding a drop of water to the drained liquid and if the water dissolves, it confirms that it is an aqueous layer). The alkyl halide was then returned to the separatory funnel. 14 mL of saturated aqeous sodium bicarbonate was added a little at a time while the separatory funnel was being swirled. A stopper was placed on the funnel and it was shaken for 1 minute while being vented frequently to relieve any pressure that was being produced. The lower alkyl halide layer was drained into a dry Erlenmeyer flask and 1.0 g of anhydrous calcium chloride was added to dry the solution. A stopper was placed on the Erlenmeyer flask and the contents were swirled until the liquid was clear. For the distillation
1. Obtain a sample of the mixture. The mixture you will separate contains three components: NaCl, NH4Cl, and SiO2. Their separation will be accomplished by heating the mixture to sub-lime the NH4Cl, extracting the NaCl with water, and drying the remaining SiO2.
In this lab or experiment, the aim was to determine the following factors of enzymes: (1) the effects of enzymes concentration the catalytic rate or the rate of the reaction, (2) the effects of pH on a particular enzyme, an enzyme known and referred throughout this experiment as ALP (alkaline phosphate enzyme) and lastly (3) the effects of various temperatures on the reaction or catalytic rate. Throughout the experiment 8 separate cuvettes and tubes are mixed with various solutions (labeled as tables 1,3 & 4 in the apparatus/materials sections of the lab) and tested for the effects of the factors mentioned above (concentration, pH and temperature). The tubes labeled 1-4 are tested for pH with pH paper and by spectrophotometer, cuvettes 1a-4a was tested for concentration and cuvettes labeled 1b-4b was tested for temperature in four different atmospheric conditions (4ºC, 23ºC, 32ºC and 60ºC) to see how the enzyme solution was affected by the various conditions. After carrying out the procedures the results showed that the experiment followed the theory for the most part, which is that all the factors work best at its optimum level. So, the optimum pH that the enzymes reacted at was a pH of 7 (neutral), the optimum temperature that the reactions occurs with the enzymes is a temperature of 4ºC or
In the experiment we used Turnip, Hydrogen Peroxide, Distilled Water, and Guaiacol as my substances. On the first activity, Effect of Enzyme concentration of Reaction Rate for low enzyme concentration, we tested three concentrations of the turnip extract, and hydrogen peroxide. For the Turnip Extract I used 0.5 ml, 1.0 ml, and 2.0 ml. For hydrogen peroxide we used 0.1 ml, 0.2 ml, and 0.4 ml. We used a control to see the standard, and used a control for each enzyme concentration used. The control contains turnip extract and the color reagent, Guaiacol. We prepared my substrate tubes separately from the enzyme tubes. My substrate tube