Enzyme Activity Lab Report

The results of the three-part experiment provide a deeper knowledge about the factors that influence the rate of the reaction of the enzyme activity and how the factors influence the structure or function of the enzyme.

The more acidic a substance is the less oxygen it will produce when going through a chemical reaction. During the Lab “How Do Changes in pH Levels Affect Enzymes Activity”, the researcher conducted an experiment to test the effects that an acidic, neutral, and a base substance will have when combine it with hydrogen peroxide. The data table shows that HCL (acidic substance) barley produced any oxygen at all when it was combining with Hydrogen Peroxide. The pH level for HCL was 2.5; this level indicates that the substance was very acidic. When the H2O and NaOH were tested they produced more bubbles than HCL. NaoH produced a little more bubbles than HCL. The pH that NaoH produced was a 9, which is a base. H2O produced more bubbles than both substances;

Enzymes are an organic substance that are made up of polymers of amino acids that help the digestive system and metabolic processes in living organisms (Funk and Wagnalls, 2016). Enzymes are able

Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is

In the initial part of the experiment, the materials used were: cuvettes, broad-range pH paper, dry watch glass, a spectrophotometer, parafilm, transfer pipette, solution E (solution B and distilled water), sodium carbonate, hydrochloric acid, and solution D (enzyme ALP high concentration). The experiment was initiated by preparing solution E. Solution E was formed by adding 6.5 mL of distilled water (dH20) with 6.5 mL of solution B (para nitro phenolphosphate - pNPP). A total of four cuvettes were labeled control 1, acidic 2, neutral 3, and 4 basic (Wilson, et al 2015). Each cuvette contains a specific pH with the exception of the control cuvette as shown in Table

Students will be observing normal catalase reaction, the effect of temperature on enzyme activity, and the effect of pH on enzyme activity in this experiment. The enzymes will all around perform better when exposed in room temperature than when it is exposed to hot and cold temperatures. This is based on the fact that the higher the temperature, the better the enzymes will perform, but as the temperature reaches a certain high degree, the enzymes will start to denature, or lose their function.

William Fung TA: Gayani Batugedara Bio 1LA, Monday 4-7pm Lab 5 Introduction: Enzymes Question #1: How does the concentration of substrate affect the reaction rate? 1. If the amount of concentration of substrate is increased, the reaction rate will be also be increased.

To test the effect of temperature, place 4, clean test tubes in a test tube rack and label them “T 0-5,” “T 20-25,” “T 30-35,” and, “T 50-55.” Add 3 mL of 3% H2O2 and 3 mL of water to each test tube. To measure enzyme activity at 0-5°C, prepare a water bath with a temperature range of 0-5°C in a 600 mL beaker. Place Test Tube T 0-5 in the water bath and record the temperature in Table 4. Add 2 drops of the enzyme solution to the test tube, and connect the free end of the plastic tubing to the connector in the stopper. Click the “Collect” button to collect data, and

Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology Department, 2011).

Enzymes are catalysts that function to speed up reactions; for example, the enzyme sucrose speeds up the hydrolysis of sucrose, which breaks down into glucose and fructose. They speed up reactions but are not consumed by the reaction that is taking place. The most important of the enzyme is the shape as it determines which type of reaction the enzyme speeds up. Enzymes work by passing/lowering and energy barrier and in doing so; they need to bind to substrates via the active. Once they do, the reaction speeds up so much more quickly than it would without the enzyme. Coenzymes and cofactors aid the enzyme when it comes to binding with the substrate. They change the shape of the active site so the substrate can bind properly and perform its function.

We then tested the last set of test tubes containing milk and lactase, we did this to find which ones would present the most glucose concentration results, when placed in different temperatures, 4°C, boiling and room temperature. What we wanted to know was how far temperature could affect lactase to perform its enzymatic activity. We hypothesized that if the lactase is placed in a high or low temperature outside its active range, the temperatures would have a negative impact on the functions of the enzyme. If the temperature has an affect on lactose then we would see some temperatures in which lactase will be function able. We came to a conclusion that enzymes work at a temperature that is closest to body temperature (25°C); boiling water (100°C) denatures the enzyme, while the enzyme is not able to function properly if

Purpose To determine the temperature at which the potatoes catabolic enzymes breakdown H2O2 (hydrogen peroxide) the fastest. Hypothesis If the temperature of the H2O2 is 35.7 degrees Celsius, then it will break down the fastest because that is the temperature enzymes work in the human body Materials -Ice Bath -Observation

Do Different Temperatures of Hydrogen Peroxide Affect Enzyme Activity? Purpose To test the activity of an enzyme called catalase when hydrogen peroxide is at different temperatures. Background Hydrogen peroxide (H202) is a chemical that is a by-product of many chemical reactions in living cells. Hydrogen peroxide is also extremely toxic to human cells.

I forecast that the more concentrated the hydrogen peroxide is the higher the volume of

Hold the IKI spray bottle 25 - 30 cm away from the paper towel, and mist with the IKI solution.