Elodea Temperature Experiment

Next prepare the third test tube with an accurate measurement of 5ml sodium carbonate once again labelling appropriately.

Pour approximately 50 mL of room-temperature distilled water into the glass beaker by using the estimated volume on the beaker.

9) Trial E: Remove the syringe and empty the beaker. Add a Thermometer to the beaker. Add 200 mL of Room Temperature water to the beaker and heat with a Bunsen Burner until it reaches 100° C. Remove the Bunsen Burner. Repeat Steps 5 & 6.

XII. Take the 250 ml beaker to your lab bench. Set up a gravity filtration with a plastic funnel, folded wet filter paper, and an Erlenmeyer flask. Pour the content in the 250 ml beaker slowly through the filter paper. Wash the filter paper with deionized water. Dispose of the filtrate in the proper labeled waste container.

After the whole set up is done we will switch on the lamps, measure the initial length of water and start the stopwatch after as we see 2 or 3 bubbles (which signify process has started).

Figure out the height the ring should be above the watch glass by hanging the (empty and clean) soda can by the tab on the stirring rod and set it up so the bottom of the can is about 2-3 inches above the watch glass (or sufficient space so the food will have enough space to burn without the hitting the can). After setting up the height of the ring on the ring stand, using the milligram scale add about water to the soda can and hang the can back up on the stirring rod. Record the mass of the water added in grams, as well as the temperature of the water in degrees Celsius which is determined using the temperature probe. Weigh the chosen food with the milligram as well and record the mass. Put the food on the watch glass and confirm the height of the ring on the in degrees Celsius ring stand is fine.

Rinse out beaker and dry it, preparing for next trial. Also reset stopwatch. Repeat steps 10-16 two more times until you have completed three trials in total. Measure the temperature of the hot water in celsius and record it to ensure consistency in temperature throughout the experiment.

mL cylinder to the beaker on the stir plate and empty it into the beaker. Place the pH probe in the beaker and record the pH in the data table. Drag the beaker to the red disposal bucket. Double-click the bottle of NaHCO3 to move it to the Stockroom counter. Repeat steps 5 and 6 for KNO3.

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

The next step in this lab is to rinse the Erlenmeyer flask with distilled water down the drain and then repeat the experiment, this time adding 10 ml of 0.10M KI and 10 ml of distilled water to the flask instead. The flask should again be swirling to allow the solution to succumb to the same temperature as the water bath and once it has reached the same temperature, 10 ml of 3% H2O2 must then be added and a stopper must be immediately placed on the flask and recording should then begin for experiment two. After recording the times, the Erlenmeyer flask must then be rinsed again with distilled water down the drain. After rinsing the flask, the last part of the lab can now be performed. Experiment three is performed the same way, but instead, 20 ml of 0.10 ml M KI and 5 ml of distilled water will be added and after the swirling of the flask, 5 ml of 3% H2O2 will be added. After the times have been recorded, data collection should now be complete.

Water can be kept a constant by keeping the same amount of water in the beaker. Temperature- Enzymes are used in photosynthesis and the respiration of the plant. Therefore, increasing the temperature will increase enzyme reaction and the photosynthetic rate until a certain point is reached when the enzymes denature. The temperature can be kept somewhat a constant by performing the experiment in one session, when the air temperature shouldn't change enough to affect water temperature. A transparent glass block will also be placed in front of the lamp to retain some of the heat from the lamp.

27. Heat the water to 40°C with an alcohol lamp by setting up the apparatus as shown in figure 3 below:

7.When water bath is ready, put each test tube into the water bath. Wait 5 minutes.

There are three main steps in preparing the experiment, which are a) experiment principle & design; b) calibration of resistance temperature detectors (RTDs) and thermocouples (TCs) and c) Set up of equipment.

What is the effect of altering the distance (±0.05 cm) of the Elodea Canadensis aquatic plant from a light source at (5cm, 15cm, 25cm, 35cm and 45cm) on the photosynthetic rate by counting the number of oxygen bubbles rising up the test tube per minute (min^(-1))?