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Ectotherm Lab Report

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Desert animals experience large daily variation in environmental temperature, with high temperatures during the day and low temperatures during the night. What effect do you expect a drop in temperature from 25°C to 0°C would have on the heart rate of an ectotherm (e.g. a lizard) compared with an endotherm (e.g. a fox)? Explain your reasoning? As the Lizard is an ectotherm, when the temperature decreases, so does the body temperature as it depends on external heat sources.This means that an ectotherm body temperature will rise when the environment temperature rises and will lower when the environmental temperature lowers. which would mean that it would effect the heart rate. This will make the heart rate slow down as the temperature has lowered. …show more content…

So how exactly does temperature affect us? (Zuo 2012) states that a change in the environmental temperature poses many potential severe problems for ectothermic organisms. Their body temperatures fluctuate with environmental temperatures and the rates of most biochemical reactions and biological processes increase approximately exponentially with temperature. So changing temperature literally changes the pace of life.

The aim of this experiment was to determine the temperature effect on metabolic rate in Daphnia. From carrying out this test we would expect there will be a difference in heart beats per minute between Daphnias kept at different temperatures as they are an ectothermic organism which means that their temperature will vary to that of the environment.

MacArthur, J.W. and Baillie, W.H.T., 1929. Metabolic activity and duration of life. II. Metabolic rates and their relation to longevity in Daphnia magna. Journal of Experimental Zoology, 53(2), …show more content…

Materials and methods

In order to safely conduct this experiment I used the following materials with great care. I used a low power microscope using up to a maximum of 40-50x magnification. A sample of several Daphnia's contained in pond water varying at temperatures of along with a pair of cavity slides to place them on. A tiny amount of cotton wool along with some tissue and a seeker pin, two pasteur pipettes to transfer the organisms, a stop watch to keep track of 0°C, 10°C,20°C and 30°C whilst using a stop watch to time the heart rate..

Before carrying out the experiment, I first thoroughly checked all the instruments over for any signs of damage or dirt as this can affect both the result and experiment itself. Using a clear cavity slide, and a pipette, I transferred a single Daphnia from one of the samples in the water at any given temperature and kept them slightly moist under water to enable them to breathe.
Once the Daphnia was in position on the slide I inserted the cavity slide under the low power microscope to observe the Daphnia's heart beat. Another member on my team held the stop watch, whilst I count the beats during the time set. A 15 second time limit was used and during those seconds I counted the Daphnia's bmp. To make things easier and also more accurate, I used a pen and notepad to mark dots on for eveytime the Daphnia's heart beated in oder to signify it as a beat and after the 15 seconds were up,I counted those dots

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