Daphnia Lab Report

For each test solution and base HR, the daphnia was submerged for ten-minutes to allow the chemicals to take effect, for daphnia are ectotherms and their body adapts to the surrounding environment. The data in Table 1 and Graph 1 answer the question of whether a daphnia, when exposed two depressants, i.e. melatonin and ethanol, will result in an average heart rate (HR) lower in comparison to the two depressants being administered individually. Over the three trials conducted for each of the test solution for daphnia 1, it can be concluded that 10% ethanol administration resulted in almost 2.6 fold, that is approximately 60%, decrease in HR. Therefore, ethanol had a depressant effect on average BPM, similar to Corotto's findings. (2010) Also, since the first daphnia died after

The purpose of the experiment was to examine the effects of certain drugs, like alcohol or caffeine, on the heart of a Daphnia magna. From what is known about our hearts, I hypothesized that the Daphnia magna would have a stimulant reaction to both alcohol and caffeine. To begin the experiment we had to prepare the organism to have a clear reading and obtain a control group. Once she was ready, we applied the drug to the organism and counted its heart beat for ten seconds, we did this procedure a total of three times for both caffeine and alcohol. As a result, the Daphnia magna caffeine treatment heart rate is considerably higher than the control heart rate and lower than the alcohol treatment.

The physiological effect of different medicines is successfully determined by counting the heart rate of Daphnia through this experiment. The data shows that different chemicals affect the heart rate of Daphnia differently. In the presence of Ephedrine, the heart rate of Daphnia has increased, and in the presence of Ethyl Alcohol and caffeine, the heart rate has decreased. The Daphnia under water is a control group, and the average heart rate is 269 BPM. The heart rate has decreased to 267.33 BPM when 0.5M Ethyl Alcohol is added and to 256 BPM when 0.5M caffeine is added.

Daphnia magna, arthropods of the subphylum Crustacea, are widely used during laboratory experiments because they are very sensitive to many environmental parameters including temperature and chemical contaminants (Cornell, 2009). In this experiment, Daphnia magna were tested under different experimental factors, including temperature changes and exposure to different chemical, in order to observe the effect of environmental conditions on their heart rate.

For the temperature treatment, it was decisive in that the A. franciscana showed a steady increase in concentration from section 1 to 4. This expands on the hypothesis that suggests A. franciscana prefers an optimum temperature between 20-24 ̊ C because from the results of the experiment A. franciscana seemed to prefer even higher temperatures. Al Dhaheri and Drew (2003) state that A. franciscana stop reproducing at temperature above 30 ̊ C and compared to the experiments results. It can be concluded that A. franciscana prefer warmer temperatures, but reproduce at lower

At first the average Heart rate of Daphnia was 22 with no treatment after measuring it for three times. Afterward when I putted 15% of Ethanol on Daphnia I found out that Ethanol does affect Daphnia and caused to decrease the heart rate of Daphnia after measuring it for three times. Daphnia’s heart rate was decreased by 7 after putting Ethanol. I would classify Ethanol as a depressant because it decreased the heart rate of Daphnia.

The following was the procedure used by the team that introduced chemicals into the environment of the Daphnia. First a zero reading was taken before any chemicals were introduced. The zero reading was an observation of the Daphnia’s heart rate before any substances were administered. All fluids were drawn off the slide using the corner of a Kimwipe. Then two drops of two percent alcohol solution were dropped onto the Daphnia. After a minute a heart rate reading was taken. The same procedure, including using the Kimwipe to draw off previous solution, was then used with four, six, eight, and ten percent solutions. A heart rate reading was taken after each solution was introduced.

In this project, C. Elegans are hermaphrodite worms that will be used since they are easy to maintain in lab, as well as have short life cycles. The gene that the project attempted to knockdown in C. Elegans with RNAi treatment is the unc-22 gene. RNAi disrupts gene expression in the presence of double stranded RNA (dsRNA) that is complementary to target gene sequence. The unc-22 gene codes for a muscle protein called twitchin in wild-type worms. The Unc-22 is required for muscle regulation and maintenance in C.Elegans. To verify that the RNAi treatment worked, would check the unc-22 mRNA levels in the worms, in addition to phenotype observation.

At the highest concentration of ethanol solution, 1x10-1gcm-3 the greatest percentage decrease of heart rate was 86.5%. The results in general show that increasing the concentration of alcohol decreases the heartbeat of the Daphnia lower. METHOD Before starting the practical part of the investigation the different concentrations of alcohol that

For the initial set-up of the experiment, two plastic beakers, each containing approximately 200mL of fish water, were individually weighed and recorded. The selected model organism for the experiment was the Carassius auratus, most commonly known as the goldfish. Each beaker was then filled with two pairs of evenly-sized goldfish and reweighed. These weights were then subtracted by the previously recorded weights to provide an approximation of the combined weights of each paired goldfishes. An oxygen chamber with a built-in probe was then filled with 200mL of fish water. The first pair of goldfish was added, along with the 200mL of fish

Immersing Daphnia in the ethanol indicated an increase in the heart rate. This relates to the fact that alcohol can have a big impact in the live of humans. Since the cross sectional part of the heart relates to that of humans. The increase in the heart rate enabled me to understand more about the how the increase in the heart rate when ethanol (Alcohol) is present in the body it can affect one's blood pressure [1]. In contrast, high blood pressure is somewhat indicates how one’s heart is beating really fast. This means that at as the heart is pumping blood through the vein and arteries in the body. The force the heart is using to pump the blood through the artery walls can cause a tear or damage of the arteries which can lead to death. Furthermore,

October 17, 18, and 19, samples were collected from multiple sites along the BSR. The class was split into groups, and samples were collected from seven separate locations along the river and WWTP. There was also a sample collected by the S which is located between sites four and five. For each of these sites, there were ten groups from other labs that also collected a sample from the BSR. At site two of the river, the location included multiple sources of possible contamination. A drainage site was located 200 yards upstream, along with a small PVC drainage pipe next to the collection site. Not only was there drainage running into the river, the site was under a bridge, and contained other trash scattered throughout the area. The

2. When 2.00 g of NaOH were dissolved in 49.0 g water in a calorimeter at 24.0 ˚C, the temperature of the

Furthermore each container had the exact same number of Daphnia magna: five Daphnia per container. 70 ml of the ammonium sulfate solution was poured into the properly labeled containers, and the five daphnia were carefully pipetted into the solution. The five containers were all out into a designated location for 24 hours. After the 24 hours, the number of remaining (alive) Daphnia were determined and charted. Data was collected for a personal group percentage and a classroom

Hypoxia is common abiotic stressor affecting the distribution and survival of aquatic species, however the occurrence varies in magnitude and spatio-temporal scale depending on the environment (Diaz and Breitburg, 2009). Due to Lauren’s arisen passion in research it led her in doing her PhD. She commenced her PhD in 2011 on the effects of mitochondria’s characteristics on a healthy and a diseased heart. Overcoming many challenges during her PhD, such that writing a supportive thesis on a drug which explicitly did not have the intended effects, which definitely changed her career choices. However, some interesting facts of her PhD incorporated; how to give a mouse a heart attack it can be done through a machine called… After completion of PhD in 2017 she was finally able to be titled Dr. Lauren, a title which is a dream of many people in the world. During her PhD she was offered to be a part time tutor for CTR in 2016 and that is where her journey to education career started and she hopes to continue it on. This was her journey that constituted of many ups and downs from the undergraduate to postgraduate level of her