Chemical Structure
It was in the year 1979 a steroid lactone which was termed as Brassinosteroid was isolated from pollen of Brassica napus (Grove et al, 1979). Later its structure was determined by different spectroscopic analysis mainly by NMR, EI-MS, FAB-MS and also by X-ray diffraction, which was found to be (22R, 23R, 24S)-2α, 3α, 22, 23, tetrahydroxy-24-methyl-β-homo-7-oxa-5α-cholestan-6-one. Since the discovery of brassinosteroid more than 69 naturally occurring compounds of this hormone family has been discovered. (Yokota 1999a) BR’s are derived from a common 5-choleston skeleton and the structural difference which is present in the different members of this hormone family mainly comes from the side chain present in A/B ring and also from different functional groups present in the ring which is shown in the figure ( Yokota, 1995, 1997).
From- The chemical characteristic and distribution of brassinosteroids in plants
Natural Occurrence and Distribution in the Plant Kingdom
Brassinosteroid molecules although present at low concentration in plants but still they have been reported throughout the plant kingdom and also they are broadly distributed from lower to higher plants. They have also found to be present in different plant organs such as flowers, anthers, seeds, pollens, roots, stems, leaves and grains, although brassinosteroid level varies among them according to organ type and tissue age. Like young and growing shoots contain higher brassinosteroids as
Ethylene produced, although is in gaseous form, can most likely add weight to the overall flower or plant. As Ag+, an ethylene action inhibitor, can bind to ethylene and
In order to generate a bicyclic lactone in this experiment, a Diels-Alder adduct was produced. The bicyclic lactone to be generated was cis-1,3,3a,4,5,7a-Hexahydro-5-methyl-3-oxo-4-isobenzofuran-carboxylic Acid and was produced using a Diels-Alder reaction. The product was also analyzed quantitatively using percent yield. To prepare the Diels-Alder adduct 0.40 g of 2,4-hexadien-1-ol was added to a flask, then 5.00 mL of toluene and 0.40 g of maleic anhydride were added to the flask in that order. The mixture was warmed and stirred to induce a reaction. The reaction progress was monitored using a TLC plate with 30:70 hexane used as the mobile phase and silica gel as the stationary phase. The TLC plate revealed a new spot for the crude product, indicating the reaction had begun.
At the start of this experiment we were required to obtain a set of four Wisconsin Fast Plants, which are genetically, known as Brassica rapa. These plants have been, “originally selected under continuous fluorescent light to grow and reproduce quickly for research purposes, these petite, fast-growing plants have been used for teaching biology concepts” (Wisconsin Fast Plants). These four pots that contain our plants will be under our watch for the next 16 weeks where we will show our results at the end of the semester.
This experiment was performed to test two hypotheses concerning the plant hormone gibberellic acid and a mutant rosette shaped phenotype of the plant Brassica rapa. This experiment was done in order to test the effects o gibberellic acid on plants and its effect on rosette shaped complexes. The two hypotheses in this experiment are as follows: Hypothesis number one states that Gibberellic acid allows for stem elongation in plants. Hypothesis number two. The rosette complex in the rosette phenotype plant contains less gibberellic acid naturally and therefore grows shorter.
Today’s lab incorporated the six steps of the scientific method to the growth and development of their own Wisconsin Fast Plant, also referred to as “Brassica rapa” in the scientific community. My group’s experiment included researching the effect of acidity on seed germination. The Wisconsin Fast Plant or Brassica rapa, was originally created by Professor Paul H. Williams at the University of Wisconsin at Madison. The word Brassica can refer to many different plants such as mustard plants, cabbages, rapes, broccoli, brussel sprouts, cauliflower, kale, kohlrabi, turnip, rutabaga, and the Chinese cabbage. The Brassica rapa plants were created to help provide a better understanding and more research on the Brassica plant’s family diseases. Brassica rapa plants are in the Cruciferae family, and are named this way because all of the plants have 4 flowers in the form of a crucifix.
In this experiment, the identity of an unknown compound will be deduced from analysis of physical properties, boiling points, IR spectra, chemical tests, and melting points of derivatives. This experiment allows for individual selection of chemical tests, order of steps, and additional analysis, as necessary, based on what is needed to determine identity of unknown.
On December 15th, 1971, the first X amendments to the Constitution went into affect. The first X amendments to the constitution were known as the Bill of Rights. The First Amendment was written by James Madison because the American people were demanding a guarantee of their freedom. The First Amendment was put into place to protect American’s freedom of speech, freedom of religion, freedom of assembly and freedom of petition. The First Amendment was written as follows;
The experiment was successfully accomplished in classroom where the Brassica Rapa were grown under suitable conditions. The experiment began by developing a watering system to the plants using the idea of capillary action. The watering system was made up of a plastic reservoir filled with a certain amount of water and a water mat placed on the top of the plastic reservoir. This design was helpful in making sure that the plants are receiving water constantly. Also, one piece of copper sulfate was addedinto the reservoir to avoid any algal growth. Two Styrofoam quads with four cells each were used for planting the seeds of Brassica Rapa. The cells were numbered from one to eight and a wick was placed in each cell in a way that allowed water access from the water mat through the capillary action. Each cell was then half way filled with soil and two fertilizers were placed in
The cell membranes are the utmost essential organelle that surrounds all living cells. Its purpose is to control what goes in and out of the cells and is accountable for the various other properties of the cells as well. The nucleus and other organelles also have membranes that are practically indistinguishable. Membranes are organised in a mosaic arrangement, comprised of carbohydrates, proteins and phospholipids. This can be seen in Figure 1. The objective of this indirect examination is to study the causes of various solvents and conducts on live beetroot cells. The reason why beetroot cells have been selected for this experiment is because they have a big membrane-bound central vacuole, as seen in Figure 2. The red colour anthocyanin, which provides the beetroot its bright colour is located in the vacuole. The cell membrane encloses the whole beetroot cell. The anthocyanin cannot leak out if the membranes stay unharmed. The red colour can escape if the membranes are hassled or broken.
The adrenal glands, located above the kidneys, are where natural hormones and steroids that the body uses are secreted and regulated. These hormones and steroids come from either the adrenal cortex, or the adrenal medulla. Hormones from the cortex start at the pituitary gland where ACTH or adrenocorticotrophic hormone is secreted. “ The adrenal cortex secretes hormones known as corticosteroids, or cortical steroids.”( Rather, Spencer) There are three zones of the adrenal cortex, the zona glomerulosa, fasciulata, and reticularis. Each of these zones produce specific steroid hormones. Two main types of steroids among many others are Cortisol and Aldosterone. Cortisol is referred to as the stress hormone as it is involved in the response to stress and anxiety. Aldosterone increases reabsorption and retention of sodium and water in the kidneys. This process involves secretion of potassium and is stimulated by low blood sodium, this increases blood volume and
Plant cells have selectively permeable membrane that allows selective substances to penetrate into the cell. Cell membranes are consisting of lipid layer that are made up of phospholipids. (Essays, UK.2013). These phospholipids are amphipathic in nature, which have phospholipids head and tail respectively. Phospholipids heads are hydrophilic and tails
This data shows a strange outcome, in the hypothesis; it says that “If acid is introduced to the seed during germination, then the roots will not grow as long as the seeds that are given water”. This statement proves to be untrue, because the roots grew longer with stronger acid than weaker acid, and in some, cases, grew better with strong acid than it did in water. This may be true because of the acid growth theory. The acid growth theory states that auxins cause the elongation of stem cells by promoting wall loosening. It was determined that this wall loosening is caused by hydrogen ions. This idea and subsequent supporting data gave rise to the acid growth theory, which states that when exposed to auxins, susceptible cells excrete protons into the wall at an enhanced rate, which in turn decreases the pH in the wall. The lowered wall pH then activates the wall loosening process which is essentially doing the same thing as the auxin hormone.
Of particular interest are those chemicals, which mimic the female hormone of estrogen, which is
The tobacco plant like many plants contain a cell callus. A cell callus contains somatic undifferentiated cells and can be used to differentiate into specialized tissues of the tobacco plant, or any plant used, by being induced with the addition of different types of hormones, such as cytokinin and auxin. Cytokinin and auxin are mostly used in plant tissue culture simultaneously to provoke the formation of a plantlet or callus. There is a common use of Kinetin in plant tissue culture since when added it will promote cell division to initiate shoot tissues from calluses of the plant. Kinetin is a type of cytokinin hormone. In regards to auxin related hormones, Indole-3-acetic acid (IAA) is also commonly used since it promotes the initiation of roots for the root tissue of the plant. In this
The class Leu, Trp, Ade dropout plates (Table 2) showed that there are interactions between the Bub1B protein produced between 186 and 613 bp on the Bub1B1 gene and CDC20 protein, as shown in Figure 1. There are interactions between the Bub1B protein produced between 328 and 588 bp and BUB3 protein. There are interactions between the Bub1B protein produced between 588 and 1052 bp and Ppp2r5c protein. There are no interactions between the Bub1B and Zfp207