The Effect of Gibberellic Acid on Wild Type and Rosette Brassica rapa Plants
Intro
This experiment was performed to test two hypotheses concerning the plant hormone gibberellic acid and a mutant rosette shaped phenotype of the plant Brassica rapa. This experiment was done in order to test the effects o gibberellic acid on plants and its effect on rosette shaped complexes. The two hypotheses in this experiment are as follows: Hypothesis number one states that Gibberellic acid allows for stem elongation in plants. Hypothesis number two. The rosette complex in the rosette phenotype plant contains less gibberellic acid naturally and therefore grows shorter.
Plant hormones are certain chemicals present in plants that control plant growth
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These not only affect the plant in their specific ways, they also have different effects when they are present in certain ratios with the other chemicals. Plant hormones maintain a delicate balance, and this experiment serves to observe that balance (Campbell and Reese, 2008).
There were two types of plants used. Both were of the same species, Brassica rapa but two of the plants contained rosette shaped complexes that were deficient in producing gibberellic acid compared to the wild-type plants. Therefore, they grew smaller and shorter as a phenotype. The Brassicaceae family includes cabbage, cauliflower, spinach, and many others. (
Hypothesis
The hypothesis behind this experiment is that the Gibberellic acid has a positive growth effect on the plant and causes it grow larger in height.
Materials and Methods
The experiment was begun by obtaining four 8 oz. Styrofoam cups and punching a hole through the bottom of them. This hole was for water entry or excess water drainage. Moistened soil was packed to the 1/2 full line in the cup along with 3 fertilizer pellets The cups were labeled the following: Rosette-H20, Rosette-GA, Wild-Type-H2O, and Wild-type- GA.(Handout 1) A small wooden applicator stick was obtained a moistened at the tip with water from the petri dish labeled ‘water.’ This was to be able to attract the seed to the applicator in order to place the seed from its original container into
After the radicles grew to about 3 cm above the soil level, I prepared 5 Gibberellic acid solutions of different concentrations. Prepare Gibberellin solution: For my experiment I will need 5 solutions of different concentrations of gibberellic acid and one control solution without any gibberellic acid added. I will repeat the experiment 5 times with each concentration.
all treatments contain the same type of soil, are planted in the same size of pan, are exposed to the same amount of sunlight, and are maintained at the same temperature throughout the course of the experiment. ON THE TEST there will be a number of related questions about this section not just the question shown below.
Today’s lab incorporated the six steps of the scientific method to the growth and development of their own Wisconsin Fast Plant, also referred to as “Brassica rapa” in the scientific community. My group’s experiment included researching the effect of acidity on seed germination. The Wisconsin Fast Plant or Brassica rapa, was originally created by Professor Paul H. Williams at the University of Wisconsin at Madison. The word Brassica can refer to many different plants such as mustard plants, cabbages, rapes, broccoli, brussel sprouts, cauliflower, kale, kohlrabi, turnip, rutabaga, and the Chinese cabbage. The Brassica rapa plants were created to help provide a better understanding and more research on the Brassica plant’s family diseases. Brassica rapa plants are in the Cruciferae family, and are named this way because all of the plants have 4 flowers in the form of a crucifix.
might affect the fitness of each variant. In other words which factors might increase plant growth, survival,
A reason as of why the experimental group grew taller would be that maybe the seeds in the control group did not get as much water as the experimental group did. Although there was a controlled amount of water put in each petri dish, there was an extra layer of paper towel on top of the seeds in the control group dish that could have soaked up more water than the experimental group. This factor could deprive the seeds of necessary water, therefore making the seed germination process much harder and could take longer. Also, there was one seed in the control group that did not germinate at all. The water could possibly not have reached that seed in particular. The water was most likely absorbed in the paper towels or other seeds before it even reached that seed in particular.
To perform this experiment we used two pots, wheat seeds, Centimeter measuring tape or ruler, labels(control and experimental) to identify different pots, water, misting bottle, compass used to know the specific direction, soil, wood sticks used for supporting the long leaves of wheat grass, and thread to fence the wheat grass.
Variables Held Constant: The length of time the bags were observed, number of trials done for each concentration and temperature and the amount of heat and light the plants were exposed to.
The experiment commenced with planting over one hundred and thirty Brassica rapa plants. A large abundance was planted to ensure the large diversity of plants, which would also ensure a number of plants would be available to artificially select. Each Brassica rapa was planted with about one hundred and thirty plants to ensure the genetic diversity but this process first commenced by accumulating small plastic pots; it was important that these pots have holes in them so that they could be filled with wick. The small piece of wick would serve as a safety net for the Brassica rapas in case they do not receive water. The wick would temporarily keep the soil moist, so the plants would not immediately face peril. We also kept the soil moist
Triggers production of other hormones, particularly ethylene and ABA, leading to leaf, abscission, abnormal growth, growth inhibition, and cell death
The control group in this experiment is the group that is grown normally to test the effects of the rest of the experiment’s independent variables. Growing Brassica rapa plants in the control group will allow the other science experiments to be compared to the normal growth of a common Brassica rapa plant. The normal growth of these plants will be important in measuring the other plants to see how their sizes compare and to show how an untreated plant would grow. Because of the nature of the control group, there is no null hypothesis since all the plants should grow at the same rate. If the correct amount of sunlight and water are given to the Brassica rapa plant, then they should grow as a normal Brassica rapa plant would. The purpose of this
The control group is the soil from the backyard while the experimental groups are the potting soil and Haw River soil. One cup of soil and five mL of Pennington Tall Fescue grass seed will be placed in each container. Three cups of waters will be given to the grass every other day and the height of the grass will be measured every week. At the end of the experiment, potting soil with 21% nitrogen had the highest grass grown. The backyard soil was the second highest grass grown with 17% nitrogen and Haw River had the shortest grass with 13% nitrogen.
This experiment studies the effect of the hormone, Gibberellic acid, on the growth of plants. The hormone increases cell growth and cell division in the stems and leaves of plants. Gibberellic acid is applied on food crops to quicken germination because it is naturally occurring and has a low toxicity (Phillips, n.d.). The purpose for studying Gibberellic acid is to observe how it affects the growth of plants. The Brassica rapa and the rosette receiving the hormone Gibberellic acid will show an increase in their health, wet mass in grams, and dry mass in grams than those that do not receive it.
The control group was the plant cuttings with no rooting hormone, just water. The experimental group was the plant cutting with the different rooting hormone brand in water. The procedures were Cut six plant cuttings off of a healthy Ruellia bush. Dip one plant cutting’s basal end in Garden Safe rooting hormone. Dip one plant cutting’s basal end in Miracle-Gro rooting hormone.
Active gibberellins show many physiological effects, each depending on the type of gibberellin present as well as the species of plant. Some of the physiological processes stimulated by gibberellins are:
Richards DE, King KE, Ait-ali T, Harberd NP. HOW GIBBERELLIN REGULATES PLANT GROWTH AND DEVELOPMENT: A molecular genetic analysis of gibberellin signaling. Annu Rev Plant Physiol Plant Mol Biol 2001;52:67-88.