Cellular Respiration Lab Report
I.Introduction
In this lab we are measuring the amount of oxygen used in both germinating and non germinating peas. We are measuring the oxygen consumption by taking a reading of a respirometer submerged in two water baths. The first bath will be cold water and the second warm to determine the effect of temperatures on oxygen consumption. Our negative control will be glass beads to measure to increase or decrease in atmospheric pressure or temperature changes. There is a direct relationship between oxygen consumption and Carbon Dioxide produced, therefore the more O2 consumed the more CO2 produced. To keep the amount of CO2 produced from canceling out any pressure gained or lost from the consumption of
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Added to the respirometers will be KOH(potassium hydroxide) to selectively remove any carbon dioxide produced with the consumption of oxygen. The three respirometers will be submerged in each bath and allowed to equilibrate. After the equilibration period the respirometers are submerged in the baths taking reading at 5 minute intervals, recording the amount of oxygen consumed in ml on the pipette attached to each respirometer.
III. Results/Presentation of Data
Our data recorded shows that the germinating peas did consume more oxygen than the non-germinating or the glass beads alone and that the cooler temperature did slow down the consumption of oxygen in the germinating peas. In both water baths the atmospheric pressure seemed to increase causing our reading to raise in our glass beads and non-germinating peas. This direct relationship in reading leads us to believe that the oxygen consumption in the non-germinating peas was minimal if any at all.
Ice Water Approx 10 Degrees Celcius Warm Water Approx 25 Degrees Celcius
Time (min) Glass Beads Germinating Peas Non-Germinating Peas Glass Beads Germinating
The first lab was conducted to analyze how germination affects the rate of cellular respiration in lima beans compared to dormant seeds. In order to
The respirometers will be prepared using two different types of cotton balls; nonabsorbent cotton and absorbing cotton. The absorbent cotton will be placed at the bottom of the respirometer and added with 15% KOH. Then, the nonabsorbent cotton
3. Explain your prediction for the effect Na+ Cl- might have on glucose transport. In other words, explain why you picked the choice that you did. How well did the results compare with your prediction?
The beads seemed to consume more oxygen than both the peas and the sunflower seeds. The amount of oxygen consumed correlates with a higher cellular respiration rate.
The hypothesis of this lab was if yeast is exposed to glucose, fructose, lactose, sucrose and DI water, then the yeast will break down glucose at the fastest rate and produce the most CO₂. This hypothesis was supported by the results of the lab. The results showed that glucose produced the most CO₂ in comparison to the other solutions, supporting the idea that cellular respiration was happening the fastest in the glucose solution. As cellular respiration is occurring, CO₂ is being released into the air. The carbon in the CO₂ is coming from carbons in the reactants of cellular respiration and are released to get rid of excess carbons. By looking at the rate at which carbon dioxide is being produced, the rate of respiration can be identified because the release of CO₂ is a product of respiration
Abstract: In this procedure, phenolphthalein will be used to detect changes in pH resulting from the production of CO2 during cellular respiration. Because phenolphthalein is red in basic solutions and colorless in acidic solutions, you can monitor cellular respiration by measuring acid production as change in pH. CO2 produced during cellular respiration can combine with water to form carbonic acid. By measuring the volume of NaOH used to neutralize the carbonic acid produced by the CO2, and thereby calculate a proximate measure of respiration.
In this activity two sets of experiments are performed to determine the rate of cellular respiration by measuring the amount of CO2 in fermentation tube. Larger the rate of cellular respiration, larger will be the amount of gas produced. To conduct the experiment yeast and water were added together at first. Yeast mixture was poured into the test tube and another test tube on the top. After flipping the tube upside down the amount of gas produced was observed at the top of Tube for about 10 minutes to determine the Cellular Respiration Rate.
This experiment discusses the rate of respiration with germinating peas, non-germinating peas and glass peas. My hypothesis that was stated prior to the experiment states that as sucrose concentration increases the time it takes for it to turn green will decrease because substrate is available. The hypothesis is supported in the experiment by the data that was collected in the experiment. For example, from 0 drops of sucrose to 6 drops of sucrose the time continuously decreased from 53.67 to 31.5 seconds. In this process of fermentation, anaerobic respiration breaks down sugars and produce CO2 and ethyl alcohol. The first phase is an energy producing phase where cells break down molecules of glucose to produce two molecules of pyruvate and
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
This experiment consisted of 3 respirometers, one with ants, one with radish seeds, and one with glass beads. Each with 4 pellets of KOH and a piece of cotton. They were placed in a water bath that was at 75 degrees fahrenheit. A bubble at the end of the respirometer was measured every five minutes, and this distance showed how well the organisms were respiring. The radish seeds were able to do the most cellular respiration in 25 minutes, with the ants being a close second, and the control respirometer of the glass beads doing the least.
Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is
What I have learned in this lab pertaining to cellular respiration and fermentation I can utilize as a nurse during respiratory therapy. As a respiratory nurse in the medical field I will have the ability to treat patients who suffer from chronic respiratory diseases like asthma, bronchitis and emphysema, understanding how cellular respiration may provide relief to someone in distress. Cellular Respiration is a metabolic process that captures the chemical energy from foods in the form of ATP. Asthma is a respiratory condition in which air flow becomes obstructed due to bronchial constriction, increased mucus secretion and inflammation The brain requires oxygen and is extremely sensitive to any reduction in the oxygen supply. Anything that interferes
Every living thing needs cellular respiration to survive. Cellular respiration is the process that releases energy by breaking down glucose and other food molecules in the presence of oxygen. This process happens through three distinct operations which are glycolysis, the Krebs cycle, and the electron transport chain. Throughout these cycles, our bodies turn oxygen and glucose into carbon dioxide, water, and energy. Although this system seems simple enough, cellular respiration can not take place in just one step because all of the energy from glucose would be released at once, most of it being lost in the form of light and heat. All this plays a very important role in our lives and without it, organisms would cease to exist.
Hand out from the Natural Science Establishment, six dialysis tubing, six plastic cups, distilled water, 0.2 M, 0.4 M, 0.6 M, 0.8 M, and 1.0 M samples of a sucrose solution, paper towels, and a scale.
The purpose of this lab is to test the effect of osmosis on cucumber slices. If a cucumber slice is placed in a hypertonic solution, then the mass of the cucumber slice will decrease. Whereas, if