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Cell Membranes Of Proteins And Proteins Essay

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Chapter One - Introduction

Cell membranes of eukaryotes are complex in structure, comprised of a highly regulated heterologous distribution of lipids and proteins (Hanada, 2010). This distribution is determined to some extent by the location and topology of lipid synthases, and results from the trafficking of proteins and lipids (Hanada, 2010). Within the cell, transport vesicles and tubules mediate trafficking by loading desired sets of proteins at one organelle and delivering them to the next (Hanada, 2010; Kumagai et al., 2005). Lipid influx routes such as the endocytosis of membrane lipids add further to the diversity (Hanada, 2010). The result is an asymmetric distribution of protein and lipid types across the membrane phospholipid bilayer (Hanada, 2010).

Ceramides are an example of a family of cellular lipids found in the cell membrane (Yasuda et al., 2001). Ceramides are synthesised at the endoplasmic reticulum from precursor compounds and are transported to the Golgi apparatus for conversion into one of several sphingolipids (Yasuda et al., 2001). The transport of such compounds is highly selective if not specific (Kumagai et al., 2005; Yasuda et al., 2001). Inhibiting transport is a useful tool in investigating the role of substrates (Yasuda et al., 2001) and makes an attractive target for biochemical manipulation of the cell (Ueno et al., 2001).

Intracellular trafficking of ceramides is highly regulated. Two ceramide transport pathways have been identified

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