Coffee is often used by many individuals as a stimulus to stay more alert when preforming tasks but it may not have the same effects on individuals with depression. The overproduction of the neurotransmitter Acetylcholine has been associated with people with depression. Active research have correlated high acetylcholine levels in the brain to increase depression like symptoms in healthy individuals and individuals with bipolar disorder. Other stimuli such as caffeine have been studied to determine an increase risked of depression by using dose-response methods in people who have experience depression in their lifetime. In this experiment the effects of acetylcholine and caffeine on the heart rate of a ghost shrimp, Palaemonetes, were tested to determine if the combination of these …show more content…
The prediction for this experiment was expected for acetylcholine to decrease the heart rate while caffeine would increase the heart rate and in combination maintain a heart rate close to normal. To obtain results the ghost shrimp was placed in water with 2000 µL of acetylcholine and the beats per minute were calculated and the same amount was used for the caffeine test. The shrimp was allowed to destress in between the tests and was finally placed in water with 2000 µL of acetylcholine and 2000 µL of caffeine. The results demonstrated that compared to the average baseline heart rate, 196 beats per minute, the caffeine increased the beats to 230 while the acetylcholine decreased the beats to 158. Together the stimuli produced an average of 200 beats per minute and produced the closest heart rate to the baseline. The statistical t test scores for acetylcholine, caffeine and in combination were 0.083, 0.029 and 0.37 respectively. These scores demonstrate that acetylcholine and the combination test were not significant enough to demonstrate a change in heart
After completing the experiment we found that when we gave the Daphnia caffeine the heartbeat rate did show an increase. However, we also found that alcohol also increased the number of times the heart beat. Even though we performed all of the experiments very carefully, we cannot be certain that the effect we saw was due to the drugs. Perhaps the change in heartbeat rate is caused by
According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing
40 2 0 Alcohol Cafeine Treatment The graph above shows that caffeine in an organism has a higher percentage than alcohol. Caffeine has an average of 148 percent, while alcohol has an average of 61 percent. Discussion As a group we hypothesized that both drugs would increase the Daplmia magna heartbeat, compared to the control group. After the results we received. I concluded that only half of my hypothesis was correct, for the alcohol actually acted as a depressant towards the
The two drugs that have been used in this experiment to test their effects on the heart rate and depolarization rate of the P wave of the crayfish. Depolarization is defined as the contractions of heart muscle, and it is shown in P wave pattern of the ECG. Comparing the results of the trials of epinephrine drug to the resting state, it shows that this drug increases the heart rate, decreases beat period, and decreases the time that take
For the experiment, crickets will be fed a certain amount of caffeine to see if it affects the crickets. The crickets that will be used are called Feeder crickets, and the caffeine that will be used is called “Walgreens stay awake caffeine tablets.”
Isolation of a Natural product, in the experiment caffeine [C8H10N4O2], is based on a series of procedures. Firstly, it is important to examine the structure of caffeine which is an alkaloid. Typically alkaloids are nitrogen based and found in plants, caffeine itself is composed of a purine ring with functional groups of a amide, amine and alkene respectively. Furthermore, Caffeine includeds a lone pair on the nitrogen atom asserting several functional properties(Zhao et al. 206). Under examination of the caffeine structure several intermolecular forces are present including hyrdogen bonding, dipole dipole forces and Van der Waals forces. What this indicates is that caffeine is a polar molecule and reacts moderatley hydrophobically in the
The following procedures and materials were thought up by my partner and me, which was done on a small scale due to lack of time to perform a full fledge experiment that involved a large sample size. Our project consisted of a sample size of ten people in which each subject was given a survey to complete and a consent form to sign stating what the experiment was about. We also expressed with the up most confidence that their scores and identities within this research will be kept confidential. We articulated to each subject to not drink any caffeine four hours prior to the experiment so that we would not have any type of bias. My partner and I obtained ten cups (regular coffee cups) on day 1 and filled each cup with 200ml of water, in which five out of the ten cups had the caffeine pill ( dissolve in water before giving to subject)
In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.
The purpose of this experiment was to identify how open and closed circulatory systems respond to chemical stimulants. During this experiment a ghost shrimp represented the open circulatory system, while the black worm represented the closed circulatory system. For each organism there resting heart rates were recorded by count the number of pulsations the organism had under a microscope. First, the shrimp and the worm were treated with caffeine, and then their heart rate was recorded after the organisms were exposed to the treatment for three minutes. The organisms were removed from the caffeine, and were given time to return back to their resting heart rates. The shrimp and the worm were then exposed to ethanol, and there hear rates were recorded again.
The drugs we used, nicotine and caffeine have a similar characteristic, both are stimulants. When nicotine is smoked it “increases heart rate, myocardial contractility, and blood pressure.”() and when caffeine is intake it “can speed up the heart rate” () in humans. For some reasons, all the data collected shows that nicotine and caffeine decreases the frequency. If we look back at tables 2 and 5, were of nicotine and caffeine were added, and the figures, it shows the drop of frequency and amplitude as acetylcholine does when is added to the heart. There is not a fully understanding of how caffeine increase the heart rate when is drunk and nicotine when is smoked, or when these two drugs are apply directly to the heart. For example medicines, medicines can have different effect if they are intake, inhale, injected or direct application to the skin. But for sure it have to do with the mechanism, the receptors and channels present on the heart. If the receptors on the heart are the causes of the decreases of heart rate, which were not taken in account on the experiment, then it would not be a surprise for any expert on the heart. Especially if we concentrate on the acetylcholine receptors. Among the different receptor, there are two kind of
One part of the human body that too much consumption of caffeine can affect negatively the your heart. Caffeine speeds up your brain cells, which speeds up your heart beat in the process. Too much caffeine will also raise your blood pressure. Caffeine using adults were polled on their stress levels given a 1 to 5 rating, with 5 being the most stressful. With each stress rating, each adult averaged a 1.7/0.4 mm Hg in blood pressure, and 1.8 beats per minute more in heart rate than adults in the lower stress rating (Lane, J.D., 2002). With further testing, they found that stress level and caffeine went hand in hand, with the highest caffeine users being the ones with 5 stress ratings. This study showed that consuming caffeinated beverages raises your blood pressure and heart rate by a considerable amount. In the same study as above, they used 500 mg of caffeine (close to 4 cups of coffee) to see how much it raised a person’s blood pressure throughout a single day. On average a person’s blood pressure was 4 mm Hg higher during than day than on a normal
The results from Graph 1 and Graph 2 clearly show that there was a very large spike in systolic and diastolic blood pressure, which occurred between the 20-30 minute marks. This pattern is consistent throughout the 5 days, which supports the research conducted by Harvard University that caffeine causes an increase in adrenaline and therefore, blood pressure in the body. It is also to be noted that the spike in systolic blood pressure increased as the dosage of caffeine increased, except for the 114mg serve of caffeine, which had a larger increase in blood pressure than the 142.5mg serve. This could have been due to the surrounding conditions of the consumer since although the starting
Sudden cardiac death (SCD) accounts for approximately one half of all cardiac deaths [Zipes and Wellens, 1998], and, although coronary artery disease (CAD) underlies most SCD events, SCD may be the first manifestation of CAD in many individuals, especially women [Albert et al., 2003]. Coffee and other caffeine-containing beverages such as tea, colas, and guarana, a soft drink made from the caffeine-rich seeds of a South American fruit pass certain kind of diseases. Many ills have been ascribed to caffeine, but few have been substantiate [Frost et al., 2005].The effect of caffeine on a disease often associate with caffeine namely, disruption of cardiac rhythm. Arrhythmias are often due to structural heart disease caused by atherosclerosis, hypertension,
The purpose of this experiment was to isolate caffeine from coffee. The coffees contain caffeine, antioxidants and diterpenes. The experiment was to extract caffeine from those compounds. Purify the inorganic compound by the process of sublimation and determine its melting point1.
In the neocortex, acetylcholine (ACh) stimulates arousal and plays a major role in attention and working memory (Jones, 2008). Neuropsychological studies on caffeine report increased arousal, alertness, increased energy and concentration (Smith, 2002; Ruxton, 2008; Brunye´ et al., 2010) even at doses as low as 50 mg, which is comparable to the amount of caffeine in a cup of tea. It has been indicated that even in low doses caffeine improves performance on attention tasks (Brunye´ et al., 2010a). However, caffeine intake has also been associated with anxiety, nervousness, irritability, insomnia and even panic attacks (Bruce et al., 1992; Lieberman, 1992; Sicard et al., 1996) . During an elevated plus maze test of anxiety in rats, it was found that high doses of caffeine brought a remarkably increased anxiety-like behavior (Jain et al., 2005).