Closed Circulatory System Lab Report

The projects purpose was to determine the effects of alcohol and caffeine on the heartbeat rate in Daphnia Magnus. Our hypothesis is alcohol causes a decrease in heart rate, whereas caffeine causes an accelerated heart rate, predicting that the more caffeine we give the daphnia the faster it heartbeat rate will become and the heartbeat rate will decrease as we give the Daphnia alcohol. After doing the experiment we found that the more caffeine we added to the Daphnia Magna the faster its heartbeat rate became. We also found that

An experiment was conducted to study and explore the circulatory system by exposing Lumbriculus variegatus, black worms, to household drugs. Lumbriculus variegatus was chosen as the experimental organism because of their transparent bodies and their simple physiology.

At first the average Heart rate of Daphnia was 22 with no treatment after measuring it for three times. Afterward when I putted 15% of Ethanol on Daphnia I found out that Ethanol does affect Daphnia and caused to decrease the heart rate of Daphnia after measuring it for three times. Daphnia’s heart rate was decreased by 7 after putting Ethanol. I would classify Ethanol as a depressant because it decreased the heart rate of Daphnia.

The following was the procedure used by the team that introduced chemicals into the environment of the Daphnia. First a zero reading was taken before any chemicals were introduced. The zero reading was an observation of the Daphnia’s heart rate before any substances were administered. All fluids were drawn off the slide using the corner of a Kimwipe. Then two drops of two percent alcohol solution were dropped onto the Daphnia. After a minute a heart rate reading was taken. The same procedure, including using the Kimwipe to draw off previous solution, was then used with four, six, eight, and ten percent solutions. A heart rate reading was taken after each solution was introduced.

The intention of the experiment was to measure how different pH levels affect the heart rate of daphnia. The objective of this experiment was to measure the heart rate of

Aim: The aim of this experiment is to understand the affect of the drug Caffeine on the rate of a Daphnia’s heartbeat. This is to understand the affect of caffeine on a human’s heart rate and the use of daphnia keeps the experiment fair and safe.

The heart serves an important purpose within the body, pumping blood throughout the circulatory system to supply all parts of the body with vital nutrients and molecules. It pumps oxygen and nutrient rich blood to be exchanged for carbon dioxide, which is then pumped to the lungs and eliminated from the body. The movement of blood throughout the body is due to the heart’s ability to push blood along the circulatory system at a steady, unfaltering rate. This rate, known as heart rate, is regulated and can be altered at a moment’s notice by signaling within the body and heart itself. In vertebrates, the autonomic nervous system controls and regulates heart rate. The autonomic nervous system is divided into two subunits, the sympathetic nervous system and parasympathetic nervous system. The parasympathetic nerve that innervates the heart is the vagus nerve. In this laboratory experiment, the regulation of heart rate was observed by studying a certain breed of turtle, the Red-eared Slider (Trachemys scripta elegans). Both chemical and electric signaling can influence the components of the nervous

Three Daphnia magna were placed in a petri dish with a small amount of spring water. Each Daphnia magna was measured separately. The Daphnia magna was placed on a slide and their control heart rate was measured for 15 seconds under the microscope and multiplied by four. Then, it was placed in the epinephrine solution for two minutes.The Daphnia magna was placed back onto the slide with the spring water and the heart rate was measured again for 15 seconds and later multiplied by four to observe the effect of epinephrine on the heart rate. They were then placed into a different petri dish of spring water after their experimental heart rate was recorded. All the heart rate values were placed into a Microsoft Excel document. The controlled variables included the temperature of the water, the time subjected to the epinephrine solution, and the time used for measuring the control heart rate and the epinephrine heart

In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.

This lab deals with the transpiration rates in plants, specifically a tomato plant that was used for this experiment. Transpiration is when water leaves a plant through the stomata as water vapor while the stomata is capturing CO2 for photosynthesis. This experiment used three different scenarios: a tomato plant with a light shining on it, a tomato plant with wind blowing on it from a fan, and lastly a tomato plant with nothing acting on it. The hypothesis is that the rate of transpiration will be fastest with light, faster with wind, and slow with the control. This hypothesis was rejected because the rate of transpiration is as follows with the wind having the fastest rate: with light the rate was 7.60 mm/min, with wind 10.20 mm/min, and control 4.33 mm/min. The cause of the wind having a faster transpiration rate than the light may have been due to the surface area of the leaves on the tomato plants. The surface area of the leaves for the wind experiment is 8,124mm2, and for the light is 7,740mm2.By doing this transpiration experiment it helps one to see what happens in plants daily and understand why it happens.

Integrin Signaling in Endothelial Cell Activation. In quiescent vessels, the endothelial layer regulates vascular tone, provides an anti-thrombotic surface, and forms a tight barrier to restrict the passage of blood components into surrounding tissue [39]. Activation of the endothelial cell layer during inflammatory responses involves a phenotypic transition that increases vascular permeability and enhances the expression of leukocyte counter-receptors (e.g. ICAM-1, VCAM-1), proinflammatory cytokines (tumor necrosis factor α (TNFα), interleukin-1B (IL-1β)), and pro-coagulant molecules (tissue factor (TF)) [39]. Molecularly, endothelial activation represents the first discernable sign of local atherosclerotic susceptibility and is maintained during subsequent stages of atherosclerotic progression [40]. Several stimuli promote endothelial activation, including mechanical stress, oxidized LDL (oxLDL), proinflammatory cytokines (e.g. TNFα, IL-1β), and the bacterial endotoxin LPS [39]. Much of the reprogramming in endothelial gene expression can be attributed to the proinflammatory transcription factor nuclear factor-κB (NF-κB) [41, 42]. The NF-B family of homodimeric and heterodimeric transcription factors is maintained in an inactive state in the cytoplasm but translocates to the nucleus upon activation in response to a variety of endothelial cell activators

Drugs rule the world, or so it appears. Many are approved for specific biological problems, as in the prevention of myocardial infarctions (heart attacks), blood clots due to trauma, surgery, stroke, etc. Conversely, others are recreational, generating instant highs or mellow lows. This essay will discuss one particular drug, chemical structure, cellular mechanism, type of inhibitor, and effects on metabolism.

The difference between anatomy and physiology is anatomy is the study of the bodily structure of other humans and animals. Physiology is the study of normal bodily functions in humans and animals. For example, anatomy is looking at the muscles and organs and Physiology is looking at the organs function and the muscles functions. The term that would most describe the pig dissection is anatomy because in the pig dissection we were looking at the different structures of the organs and muscles inside the pig while if the term was physiology we would be looking at the organs working. Homeostasis is the tendency of an organism to regulate its internal conditions, usually by a system of feedback controls, to stabilize health and functioning, regardless

The recent wealth of data should give us more confidence in a cause and effect relationship, but we are not nearly to the point of "proof." It took decades and hundreds of studies before the Surgeon General was willing to declare that smoking causes cancer.] But it has been less clear just how alcohol works to protect the body against heart disease and death. A new study from researchers at the University Hospital of Zurich, Switzerland. identifies a mechanism for how alcohol favorably effects arterial muscle cells. According to Wilhelm Vetter, M.D., and colleagues, alcohol, when consumed around mealtime, reduces the proliferation of smooth muscle cells (SMC) within the arteries. SMC growth is a key element in the development of atherosclerosis, which commonly leads to heart attacks and strokes.

Environmental pollution has become a rising problem around the world with an increase of combustion of fossil fuels becoming more prevalent in the past 100 years. Copious amounts of menacing chemicals, through these processes, thus have been able to contaminate the environment causing adverse effects on not only the environment itself, but on human health too (Kampa and Castanas 2008). Daphnia Magna are miniscule crustaceans with qualities that are key in the laboratory: they are economically cheap, low maintenance, and transparent. Because of these properties, Daphnia are essential in being able to see the effects of chemical agents on heart rate (Corotto et al. 2010). Furthermore, Daphnia may also serve as a model system to help aid us in measuring the effects of contaminants on Daphnia just as we would in humans. In this experiment we analyzed the heart rate of the Daphnia when given caffeine, ethanol, vinegar, triclosan, and sodium phosphate in order to explore how Daphnia magna behaved when exposed to potential contaminants.