Bos Taurus Tissue Essay

The three tissues being analyzed in this experiment, those of the kidney, heart and liver, were taken from the animal Bos taurus. The tissue homogenates used were made by adding 1 gram of tissue to 20 ml of sucrose phosphate buffer. The buffer was composed of 250 mM sucrose, 50 mM NaPO4, with a pH of 7.4. This mixture was homogenized with a high-speed blender for 3 pulses for a duration of 10 seconds each. The homogenates were then filtered through a cheesecloth and stored at -70° C.

Glucose test was done by inoculating one glucose tube with the bacteria in the

Abstract: This lab was developed to investigate blood glucose and diabetes. Diabetes is a lifelong chronic disease in which there are high levels of sugar in the blood (Diabetes). The spectrophotometer was applied to this lab to determine the absorbance of blood glucose in diabetic and non-diabetic blood samples. In order to prove this, tests were conducted by taking the blood samples at different times right before a meal was eaten then 30, 60, 90, and 120 minutes after the meal. The 6 test tubes had been placed in the spectrophotometer to measure the absorbance of blood glucose in the diabetic and non-diabetic blood. It was hypothesized that people with diabetes will absorb more

Incorporation of assay controls included setting up a spectrophotomer and running the chart recorder with a full-scale deflection before the start of the assay. The set recorder had a corresponding value of 1 for the change in the absorbance. Therefore, prior testing was done to observe whether a change occurred in the readings. This helped to indicate that the results were valid, as they could have been affected by a fault during the setting up of the spectrophotometer. On the other hand this was considered as one of the controls for the experiment. Nevertheless, a new cuvette had to be used for each assay.

Gelatin is observed to have a lower absorbance reading than lysozyme in only the Bradford assay, while in the BCA assay gelatin was observed to have about the same absorbance as lysozyme. In Bradford assay, the color yield for proteins with higher content of tryptophan, tyrosine, or cysteine residue, will be higher. The observed protein sensitivities did not correlates with the amino acid content in the Bradford assay. As presented in Table I, the mole fractions of lysine and arginine in BSA were 10 mole percent and 4 mole percent, respectively. The sensitivity should have been higher in BSA than in the Bradford assy. However, the observed sensitivities did correlates with the amino acid contents in the BCA assay.

Blood samples were drawn between 7 and 10 a.m. after the fast, waited for the coagulation, and centrifuged for 15 min at 1,500g in the room temperature. Each serum was then aliquoted, stored in the -70 o C storage, and used for the assay of glucose,

The absorbance is measured using a Plate reader and a Standard curve is generated. Also, the different types of pipetting techniques are assessed in this Assay.

Six beakers were obtained and each labeled separately as yeast, glucose, sucrose, maltose, fructose, and pyruvate. First, the 5.4% w/v solution of Saccharomyces cerevisiae yeast was created by measuring 5.4 grams of the yeast (obtained from Fleischmann's RapidRise highly active yeast) and adding it to 100 mL of distilled water in the beaker labeled yeast. Next, the 20% w/v solutions of each substrate were created. For glucose, 10 grams of glucose (obtained from VWR International /BDH, item # BDH0230) was measured and added to 50 mL of distilled water in the beaker labeled glucose. For sucrose, 10 grams of sucrose (obtained from Carolina Biological Supply Company, item # 89-2860) was measured and added to 50 mL of distilled water in the

The experiment aimed to determine the effectiveness of differential centrifugation and tissue homogenisation methods of separating and purifying subcellular organelles from liver tissue. Effectiveness was determined by assaying for the activity of marker enzymes specific to organelles in each fraction and calculating the specific activities and percentage distribution of each enzyme in each fraction (Watson, 2016).

First, four test tubes were labeled I for iced, B for boiling, 37 for the 37°C water bath and R for room temperature. Four cubes of liver were dissected into 12 cm3 and the weight was tared to 0.5g. The weighed livers were added into the test tubes labelled I, B, 37 and R. Deionized water was added to the liver labelled B and 37. The test tube labelled B was subjected to a boiling bath for a minimum of 30 minutes. The liver was tested when it cooled down.

Once the solutions were made, we determined the isotonic and hemolytic molar concentrations of NaCl and glucose using sheep red blood cells (RBCs). Using the previous molar solutions, we added the sheep RBCs to each solution. Later, we transferred each mixture to a cuvette and determined the absorbance of each by using a spectrophotometer. In addition,

The purpose of this experiment is to test and measure the time taken for blood glucose levels to rise after an increase in amount of glucose in the blood, and then the time taken for blood glucose levels to then return to within a normal range. This will be done by using lucozade to elevate blood glucose levels. It is then expected that the blood glucose levels should then return to within the normal range, which in a healthy person should be around 5mmol/L. The rate at which the blood glucose levels return to the normal range depend on the cells ability to absorb the glucose from the blood, which depends on the synthesis and use of insulin. The same test will be carried out with water instead of lucozade in order to obtain

Glycerol, or 1,2,3-propanetriol, is a trihydric alcohol which is also known as Glycerine, Glyceritol, Glycyl alcohol, Trihydroxypropane, Propanetriol, Osmoglyn, and 1,2,3-trihydroxypropane (Figure 1.2). The general properties of glycerol; it is colourless, odourless, sweet in tasting and in the form of syrupy liquid. It will melts at 17.8 °C and boils with decomposition at 290 °C, also soluble in water and ethanol. The molecular formula of glycerol is: C3H8O3 and its molecular weight is: 92.09382

Bradford protein assay was conducted to evaluate protein concentration in the extracted liver. The presence of peptide bonds reflect the content of protein in

some of the test that have been done propose that glucose polymers such as maltodextrin are absorbed quicker and use more, than