Boomerang Lab Report

In this project, C. Elegans are hermaphrodite worms that will be used since they are easy to maintain in lab, as well as have short life cycles. The gene that the project attempted to knockdown in C. Elegans with RNAi treatment is the unc-22 gene. RNAi disrupts gene expression in the presence of double stranded RNA (dsRNA) that is complementary to target gene sequence. The unc-22 gene codes for a muscle protein called twitchin in wild-type worms. The Unc-22 is required for muscle regulation and maintenance in C.Elegans. To verify that the RNAi treatment worked, would check the unc-22 mRNA levels in the worms, in addition to phenotype observation.

My goals for this project are to have fun, build a successful glider, and try to win. I am a very competitive person and I will do my best to try and show it. I’m hoping through trial and error that I will achieve my goals.

1. Introduction Mould is a multi-cellular organism related to the fungi kingdom. It is also related to yeast and mushrooms. It lives off dead matter and develops when there is an adequate amount of food. The mould then secretes a slime coat to help break down the food.

For the rocket we build the nose of the rocket first. To make the nose we cut the top portion out of the second two liter bottle. Then we will need to cut out a slit on the bottle in the shape of a rectangle. We made sure to leave one side attached to the bottle. We cut the very top of the nose and replaced the top with a half of a ping-pong ball for the tip of the nose. Then after the nose was dried we secured the ping pong with duct tape. In the inside we took the balled up clay and glued it down to the inside of the nose for stability . Then we started to make the wings. We cut the 2 large taperd fins out of the foam board and 2 smaller tapered fins . We then attached the wings that were covered in red duct tape for decoration to the

Data were collected in September and November 2017 at the Elk Valley Preserve and Field Station in Banner Elk, NC. The preserve is located at 36°10'17.2"N 81°54'45.9"W, in Avery County in western North Carolina. The area is a mixed deciduous forest at an altitude of 1,127 m above sea level. Data were collected in early fall, during which temperatures average around 10-18 degrees Celsius. Summers in Banner Elk are typically mild, with temperatures averaging 21 degrees Celsius, and winters are cold and snowy, averaging near zero degrees Celsius. During the months of September and November there is an overall average precipitation of 12-25cm.

Since their biological and medical application is important in discovering possible diseases, cures, and evolutionary relationships, the prevalence and spread of bacteria and fungi were studied based on the species richness, diversity, and relative abundance. Specifically, ten cell phone surface samples were taken and measured based on two parts: the outside of the phones and the inside of the cases, and the front of the phones and the back of the cases. In this lab, the front of the phone and the back of the case was hypothesized to contain species with more richness, diversity, and relative abundance. In order to test the hypothesis, ten Petri dishes were divided into two sides, five dishes meant for the front of the phones and the back of

Abstract. Despite decades of research, a prebiotically realistic scenario for the spontaneous polymerization of mononucleotides to form RNA has not yet been demonstrated. This is, in part, due to the difficulty in forming multiple phosphodiester linkages in an aqueous environment. Implicit in this approach is the assumption that extant biomolecular structures, such as RNA, were produced directly from prebiotic chemistry. A new approach considers RNA as a product of evolution, with more chemically accessible genetic polymers preceding it.

phenylethyl alcohol to change the membrane permeability of Gram-negative bacteria (13). Growth on the plate indicated a positive result. Lack of growth on the EMB plate also suggested a Gram-positive bacterium. This was because the medium contained a methylene blue dye that hindered the growth of such bacteria (14). The high sodium content of the MSA plate created a high osmotic pressure that inhibited the growth of species that cannot withstand the pressure (15).

Silibinin from Sigma-Aldrich (St Louis, MO, USA), terbium (III) chloride hexahydrate (TbCl3.6H2O) from Acros Organics (New Jersey, USA), 2-amino-2hydroxymethyl-propane-1,3-diol-hydrochloric acid (Tris-HCl) and hydrochloric acid and methanol from Scharlau (Barcelona, Spain) was purchased. Deionized distilled water was provided from Shahid Ghazi Pharmaceutical Company (Tabriz, Iran). 1000 mg/L of silibinin stock standard solution was prepared in methanol after sonication for 10 min. The working solutions were prepared daily with appropriate dilution with water. Terbium (III) chloride hexahydrate stock solution (5× 10-2 M) was prepared by dissolving 0.465 g of TbCl3.6H2O in 25 ml water and diluted by distilled water to prepare working solutions.

Materials and Methods H2 production from sunlight and hydrolyzed molasses by Anabaena variabilis Anabaena variabilis ATCC 29413 grown in an 8- fold dilution of the medium of Allen and Arnon (AA/8) [17] was re-suspended up to a final optical density (OD750 = 1) in nitrogen deprived medium supplemented with 2gl-1 reducing sugars of acid hydrolyzed molasses in sealed cultures (20 L). The culture was sparged with nitrogen gas for 1 h and left in dark overnight in an open field. The photofermenter was thus subjected to day, night flacuations for up to 3 days. The temperature was kept at 30 °C and hydrogen production was followed by collecting the gas in a collection system described previously [18].

Testing antibacterial soap and regular soap to see how much bacteria is left behind on golf balls Introduction: This study will show us if using antibacterial soap is better for us. Most people spend the extra dollar or two to buy antibacterial soap to ensure they are getting rid of bacteria. Does antibacterial soap really work better than regular soap? Do you actually have to spend the extra money to ensure a good clean? This study may answer those questions for you.

4.0 Materials and Methods 4.1 Preparation of Plants Water spinach (Iponomea aquatica) and water mimosa (Neptunia Oleracea) will cultivate for 30 days in Hoagland nutrient solution in the lab. This solution play its role as a universal nutrient solution or commonly known as base solution for plants growth in hydroponic environment, which is growing environment of plant using water instead of soil. The nutrient solution will contain : 4.1.1 Ammonium nitrate (NH4NO3), 38mg/L 4.1.2 Potassium dihydrogen phosphate (KH2PO4), 3.5 mg/L 4.1.3 Potassium chloride (KCl), 30 mg/L 4.1.4 Calcium Chloride (CaCl2.2H2O), 9 mg/L 4.1.5 Magnesium sulphate heptahydrate (MgSO4.7H2O), 7 mg/L 4.2 Plant Treatment with Heavy Metals After the Hoagland nutrient solution

Gather the necessary tools for the job. You will need a racecar. Make sure the car is clean. You also need painter's tape which can be found at any Canadian Tire for $3.69. You also require a variety of brushes, one 1/2 inch roller brush, a 3 inch roller brush, and a 2 inch polyester

Studies show that unwashed or lightly rinsed vegetables can harbor pathogenic bacteria and have been implicated in numerous foodborne infections. The objective of this experiment was to obtain a quantitative viable plate count of enteric bacteria present in a sample of broccoli sprout water. To enumerate the number of bacterial colonies present in the sample, we performed serial dilutions of broccoli water spread onto MacConkey Agar plates. MacConkey Agar is a selective and differential media. It is used to select enteric bacteria and differentiate lactose-fermenters from non-lactose fermenters. Utilizing this process illustrated the potential amount of foodborne illness causing bacteria present on foods, specifically fresh broccoli

The successful flight of a boomerang looks as though it never should happen. Its more or less circular flight path comes from the interaction of two physical phenomena: the aerodynamic lift of the arms of the boomerang and the spinning boomerang’s maintenance of angular momentum. Briefly put, the airfoil at the boomerang’s forward rotating edge provides more lift than its rearward rotating edge. This elevates one side of the boomerang. The spinning object maintains angular momentum by turning at a right angle to its axis of rotation. When the spin and the velocity of boomerang are just right, it flies away and returns in an aesthetically satisfying circle.