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Animal Respiration Lab Report

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The Effect of Temperature on Animal Respiration
Renee King 001420538
TA: Oliver
Biology 3U03
L01

This lab examined the effect of temperature on the rate of oxygen consumption by measuring VO2. Effects were observed in goldfish, frogs, and mice, which each use different mechanisms for thermoregulation. The average rate of oxygen consumption by goldfish increased from an average of 0.175 mL g-1 h-1 at 5֯ C to 0.288 mL g-1 h-1 at 25 ֯C which was a 64% increase. The average rate of oxygen consumption by the frog was higher at 5֯ C than at 25 ֯C. At both temperatures, the rate of oxygen consumption increased over time. The results found in the frog were also very similar to the results found in the mouse. The average rate of oxygen …show more content…

As shown in Figure 1, there was a 64% increase in oxygen consumption at 25 ֯C compared to 5 ֯C for the goldfish, and therefore the hypothesis was correct. Many cues are known to alter the morphology of the gill epithelium in fish. The goldfish gill contains lamellae, thin tissue plates, which are highly functional in temperatures above 15 ֯C. When the fish is subjected to colder water, usually below 8֯, the lamellae become lodged as one large mass of cells. The covering of the lamellae in fish in colder water increase surface area for gas exchange and thus a high breathing rate is not needed. When the fish is in warmer temperatures, this cell mass is removed, thus exposing the lamellae and increasing the need for a high breathing rate. An increase in temperature also acts as a cue for fish to lose the cell mass, which may be related to the higher demand for O2 as the metabolic rate must increase to support this process (Tzaneva et al., 2011). Since goldfish are cold-blooded, they depend on their outside environment to regulate body temperature, and are not largely affected by major temperature changes (Guyton & Hall, …show more content…

In a behavioral hypothermia study done by Tattersall & Boutilier (1997), behavioural hypothermia responses were observed in bullfrogs to show the advantages of maintaining oxygen levels during hypoxia. During the winter months, frogs hibernate and during this time metabolic rate largely decreases as they live on the bottom of ponds in very hypoxic water. However, these adaptations are only seen long term. As shown in Figure 2, oxygen consumption increased over time at 5֯ C. Therefore, it is expected that oxygen consumption in frogs would decrease over a longer period of time. In addition, frogs in cold water have a low blood PCO2 due to the ability to lose carbon dioxide through the skin and uptake more oxygen. This is why the rate increased much more quickly than in the mouse (Figure 3) at 5֯ C. This increased metabolic rate and VO2 during this short period of time in cold water is expected. Breathing rate also increased in 25֯C water as expected. As temperature increases, carbon dioxide levels increase and therefore breathing rate must increase to bring in more oxygen and release carbon dioxide. This rate increases relatively quickly due to the ability to breathe through the skin and mouth lining (Tattersall & Boutilier, 1997). It was difficult to measure ventilation rate, as the mL of water measured in the tube was not directly proportionate to

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