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36 Mrsa Case Study

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Results
Samples collection and primary identification
A total of 88 S. aureus were isolated from different samples including surfaces (n=34, 38.9%), personnel (23, 26.6%), air (20, 23%) and patients (11, 11.9%).
A total of 36 (40.9%) MRSA were found, of which 26 (72.2%) were isolated from surfaces, 5 (13.8%) from indoor air, 4 (11.1%) from staff and one (2.7%) from a patient. All the 36 MRSA isolates were both mecA positive and cefoxitin resistant. Antibiotic susceptibility
The antimicrobial susceptibility patterns of S. aureus (MRAS and MSSA) isolates to various antibiotics are presented in Table 1. All S. aureus isolates were susceptible to vancomycin. Quinupristin-dalfopristin, linezolid and minocycline with resistance rates of 1.1%, …show more content…

The predominant AME genes were acc (6')-Ie-aph (2'')Ia (n=25/40; 62.5%), aph(3'')-IIIa (n=12/40; 30%), acc(6')-Ie-aph(2'')Ie (n=11/40; 27.5%), and ant(4')-Ia (n=6/40; 15), among which co-existence of two genes included 35% (n=14/40) of the isolates. According to Table 3, all gentamicin resistant MRSA and MSSA isolates harbored at least one AME gene. However, no other AME genes were detected in the study. The msrA gene was more distributed among macrolide and lincosamide resistant isolates. MsrA was found in 46.8% (22/47) of erythromycin resistant, 46.6% of clindamycin resistant, and 36.3% of erythromycin and clindamycin resistant …show more content…

As shown in Table 4, virulence determinants were more distributed among MSSA isolates. The eta exfoliative toxin was also found in 22.2% of MRSA isolates.

SCCmec typing
Of 36 MRSA isolates, 11 (30.5%) were identified as SCCmec type I, nine (25%) as SCCmec type II (25%), eight as SCCmec type IV (22.2%) and three (8.3%) as type III (8.3%). Five (13.8%) isolates were also not identified as any SCCmec types. Distribution of major SCCmec types according to antibiotic resistance was shown in Dendrogram 1 and Table 5.

Pulsed-field gel electrophoresis (PFGE) PFGE results showed 33 different pulsotype patterns designated 1-33, among which 20 patterns had more than one S. aureus isolate and 13 patterns had only one isolate. The major pulsotypes among MRSA isolates were 15 (6/33; 18.1 %), 6 (6/33; 18.1 %), and 1 (4/33; 12.1 %). Distribution of major pulsotypes according to antibiotic resistance was shown in Table 5. Of 33 pulsotype patterns, 20 patterns had more than one S. aureus isolate and 13 patterns were consisted only of one isolate of this bacterium. Pulsotype patterns 15, 6, 8, and 1 had the highest abundance which detected in 14, 8, 6 and 6 strains of isolated S. aureus, respectively. The pulsotype patterns with the lowest abundance included patterns 3, 7, 9, 10, 11, 14, 17, 13, 20, 21, 25, 23, 22, and 30, each of which was consisted of one strain of S. aureus. Also, 8 pulsotypes in 11 samples

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