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Mastitis Milk Paper

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Out of the 93 contagious clinical mastitis milk samples examined, 54 (58.1%) S. aureus were identified as the causative agent of mastitis. The number of the coagulase positive (CP) and coagulase negative (CN) S. aureus were 46 (85.2%) and 8 (14.8%) respectively.
3.2. Total S. aureus counts in the milk samples
The mean counts of S. aureus recovered from the 54 S. aureus positive mastitic milk samples examined were between 8.6 × 104 ± 3.5 × 105 CFU/ml. 3.3. Detection of MRSA and VRSA isolates by the Kirby-Bauer disc diffusion method
The eight CN S. aureus isolates were neither resistant to cefoxitin nor vancomycin. Out of the 46 isolated CP S. aureus, 7 (13%) isolates were resistant to both cefoxitin and vancomycin (MRSA + VRSA) while 12 (22.2%) …show more content…

3.4. MIC of Graded Concentration Oxacillin and Vancomycin antibiotic strips against MRSA and VRSA isolates
All the previous identified MRSA and VRSA isolates by the disc diffusion method were subjected to the graded concentration oxacillin and vancomycin antibiotic strips examination, respectively; and the obtained MICs were >256 µg/ml for both oxacillin and vancomycin against MRSA and VRSA respectively.
3.5. Molecular identification of MRSA using polymerase chain …show more content…

Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) Determination of the D. angustifolia, Gold Nanoparticles, Honey against MRSA and VRSA isolates
Figure 2 (A) shows that D. angustifolia plant extract didn’t produce any anti MRSA or VRSA effect since the bacteria remain viable for up to 48 hours. However, figure 2 (B-D) shows that the gold nanoparticles and Imtenan® citrus blossom honey both in a alone form and in mixed form have anti MRSA and VRSA effect and the MIC, MBC values, and MBC/MIC ratios of the them are presented in Table 1
3.6. Statistical analysis
The statistical analysis of the MIC and MBC of each antimicrobial agent against two group of bacteria (MRSA+VSSA and MRSA+VRSA) were represented in terms of minimum, maximum, median, and P value as represented in table 2 and 3 and diagram 1- 4. Table 2 and 3 describe also the Mann-Whitney test (none parametric test) between each antimicrobial agent in alone form and in mixture form.
The statistical analysis showed significant difference between the effect of the Imtenan® Citrus Blossom honey in alone form and in the mixture form on the growth inhibition of MRSA+VSSA with the MIC P value was 0.017 (<0.05) as represented in table 3 and diagram 3 and

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