Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 12.13, Problem 1MQ
Summary Introduction
Recombinant DNA technology is to create genetically modified organisms (GMOs). Genetically engineered plant, animal, microorganism contains a specific gene (or genes) derived from other organisms is known as transgene. These genetically engineered organisms are called as GMOs. GMOs are used to produce various products for human needs.
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Chapter 12 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
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- (a) What do reverse transcriptases do? Give two examples of these and what they do in the cell. (b) What is an auxotroph and how are these obtained and isolated for study? please give answer for both questions thank youarrow_forwardDescribe how replica plating is used to detect and isolate auxotrophic mutants.arrow_forwardGenetically modified foods are products produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering. Golden rice, Flavr Savr tomatoes and Roundup Ready soyabeans are products of this technology. Discuss the advantages of these three genetically modified crops. Name ONE (1) strategy to produce transformed plant cells.arrow_forward
- Explain about auxotroph. Define its working ?arrow_forwardWhat are the advantages and disadvantages of Development of genetically modified crops that are resistant to pests and Drug delivery systems that are based on bacterial or viral host.arrow_forwardWhat makes the species of Agrobacterium ideal for genetic engineering? Describe its characteristics and its role in producing transgenic plants.arrow_forward
- Your supervisor asked you to insert a specific stress-tolerant gene into a tissue cultured plant batch. What can be your possible approach? You may only give a flow chart to show your process.arrow_forwardInsert a specific stress-tolerant gene into a tissue cultured plant batch. What can be your possible approach? You may only give a flow chart to show your process.arrow_forward1) Complementation occurs when auxotrophic organisms provide a wildtype allele that another auxotroph is lacking to produce a needed molecule for a chemical pathway. The wt allele can be passed on through horizontal gene flow, or the interaction of the auxotrophs during reproduction produces an offspring with a 'rescued' phenotype due to independent assortment returning favorable recombination of function alleles. If a researcher interacts four auxotrophs, and mutant -1 complements mutants -2, -3, and -4, and mutant -2 complements mutants -3 and -4, and mutant -3 complements -4, how many genes are indicated? a) Four genes! b) One gene with four alleles! c) Three genes with one allele. d)A complementation chart was not provided so I cannot answer this question. 2) An inheritance ratio of 12:3:4 is a sure indication of: a) Incomplete assortment. b) A dihybrid example of codominance. c) Dominant epistasis. d) Recessive epistasis.arrow_forward
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