Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement or during the transfer to the spectrophotometer?
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Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?
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Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement or during the transfer to the spectrophotometer?
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- What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700
- If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints, and dust prior to reading it in the spectrophotometer, how might the absorbance value of the sample be affected? (Higher, Lower, No Change) ExplainExplain the relationship between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.What solution goes into the cuvette when you’re zeroing the spectrophotometer?
- The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.
- In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.What is the magnification of the following drawing of a specimen extending one- third of the medium power field (1.8mm) of a compound microscope? 10.8 cmWhat is the stationary and mobile phase of spectrophotometry?