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In spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?
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- What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?What is the stationary and mobile phase of spectrophotometry?
- if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?WHAT ARE THE Steps in spectrophotometer?The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?
- the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's LawWhat solution goes into the cuvette when you’re zeroing the spectrophotometer?
- Some spectrophotometers use wavelengths of light in the ultraviolet range. What are wavelengths of light that are in the ultraviolet range?What are the light sources used in UV-vis spectrophotometry.Why does one see methyl orange as orange in color? What blank would be used to standardize this spectrophotometer?